LOCUS       BBa_K389052             2332 bp    DNA     linear       12-May-2024
DEFINITION  Tightly regulated lacO + mRFP
ACCESSION   BBa_K389052
VERSION     BBa_K389052.1  
FEATURES             Location/Qualifiers
     misc_feature    1..37
                     /label=P(Lac) IQ
     promoter        3..8
                     /label=-35
     promoter        26..31
                     /label=-10
     promoter        1..37
                     /label=BBa_I14032
     misc_feature    50..53
                     /label=conserved
     RBS             46..57
                     /label=BBa_B0034
     misc_feature    1153..1191
                     /label=LVA
     CDS             64..1191
                     /label=lacI-LVA
     misc_feature    64..1191
                     /label=BBa_C0012
     misc_feature    1192..1216
                     /label=Help:Barcodes
     CDS             64..1191
                     /label=BBa_C0012
     stem_loop       1236..1279
                     /label=stem_loop
     misc_feature    1225..1304
                     /label=BBa_B0010
     terminator      1225..1304
                     /label=BBa_B0010
     stem_loop       1324..1367
                     /label=stem_loop
     misc_feature    1313..1392
                     /label=BBa_B0010
     terminator      1313..1392
                     /label=BBa_B0010
     terminator      1225..1392
                     /label=BBa_B0017
     CDS             1401..1488
                     /label=end of LacI coding region (inactive)
     misc_feature    1401..1600
                     /label=BBa_R0010
     misc_feature    1489..1526
                     /label=CAP binding site
     promoter        1537..1542
                     /label=-35
     promoter        1561..1566
                     /label=-10
     misc_feature    1566..1600
                     /label=LacI binding site
     misc_feature    1573..1573
                     /label=start
     promoter        1401..1600
                     /label=BBa_R0010
     promoter        1..1600
                     /label=BBa_K389050
     misc_feature    1613..1616
                     /label=conserved
     RBS             1609..1620
                     /label=BBa_B0034
     CDS             1627..2301
                     /label=mrfp1
     misc_feature    2308..2332
                     /label=Help:Barcodes
     CDS             1627..2332
                     /label=BBa_E1010
ORIGIN
        1 tggtgcaaaa cctttcgcgg tatggcatga tagcgcctac tagagaaaga ggagaaatac
       61 tagatggtga atgtgaaacc agtaacgtta tacgatgtcg cagagtatgc cggtgtctct
      121 tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa
      181 aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc acaacaactg
      241 gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct gcacgcgccg
      301 tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag cgtggtggtg
      361 tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg
      421 caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc cattgctgtg
      481 gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca gacacccatc
      541 aacagtatta ttttctccca tgaagacggt acgcgactgg gcgtggagca tctggtcgca
      601 ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg
      661 cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat agcggaacgg
      721 gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct gaatgagggc
      781 atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc
      841 attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata cgacgatacc
      901 gaagacagct catgttatat cccgccgtta accaccatca aacaggattt tcgcctgctg
      961 gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt gaagggcaat
     1021 cagctgttgc ccgtctcact ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc
     1081 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     1141 gaaagcgggc aggctgcaaa cgacgaaaac tacgctttag tagcttaata actctgatag
     1201 tgctagtgta gatctctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     1261 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag agccaggcat
     1321 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     1381 gtgaacgctc tctactagag caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     1441 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     1501 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     1561 tatgttgtgt ggaattgtga gcggataaca atttcacaca tactagagaa agaggagaaa
     1621 tactagatgg cttcctccga agacgttatc aaagagttca tgcgtttcaa agttcgtatg
     1681 gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg tcgtccgtac
     1741 gaaggtaccc agaccgctaa actgaaagtt accaaaggtg gtccgctgcc gttcgcttgg
     1801 gacatcctgt ccccgcagtt ccagtacggt tccaaagctt acgttaaaca cccggctgac
     1861 atcccggact acctgaaact gtccttcccg gaaggtttca aatgggaacg tgttatgaac
     1921 ttcgaagacg gtggtgttgt taccgttacc caggactcct ccctgcaaga cggtgagttc
     1981 atctacaaag ttaaactgcg tggtaccaac ttcccgtccg acggtccggt tatgcagaaa
     2041 aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg tgctctgaaa
     2101 ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc actacgacgc tgaagttaaa
     2161 accacctaca tggctaaaaa accggttcag ctgccgggtg cttacaaaac cgacatcaaa
     2221 ctggacatca cctcccacaa cgaagactac accatcgttg aacagtacga acgtgctgaa
     2281 ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct agtgtagatc gc
//