##gff-version 3
##sequence-region BBa_K389052 1 2332
BBa_K389052	.	ribosome_entry_site	1609	1620	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K389052	.	conserved_region	1613	1616	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K389052	.	promoter	1	1600	.	+	0	ID=BBa_K389050;Name=BBa_K389050
BBa_K389052	.	ribosome_entry_site	46	57	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K389050
BBa_K389052	.	conserved_region	50	53	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K389052	.	promoter	1	37	.	+	0	ID=BBa_I14032;Name=BBa_I14032;Parent=BBa_K389050
BBa_K389052	.	operator	1	37	.	+	0	ID=annotation1028342;Name=P(Lac) IQ;Parent=BBa_I14032
BBa_K389052	.	promoter	26	31	.	+	0	ID=annotation1028344;Name=-10;Parent=BBa_I14032
BBa_K389052	.	promoter	3	8	.	+	0	ID=annotation1028343;Name=-35;Parent=BBa_I14032
BBa_K389052	.	CDS	64	1191	.	+	0	ID=BBa_C0012;Name=BBa_C0012;Parent=BBa_K389050
BBa_K389052	.	engineered_tag	1192	1216	.	+	0	ID=annotation2213988;Name=Help:Barcodes;Parent=BBa_C0012
BBa_K389052	.	tag	1153	1191	.	+	0	ID=annotation1722;Name=LVA;Parent=BBa_C0012
BBa_K389052	.	CDS	64	1191	.	+	0	ID=annotation1723;Name=lacI-LVA;Parent=BBa_C0012
BBa_K389052	.	engineered_region	64	1191	.	+	0	ID=annotation7031;Name=BBa_C0012;Parent=BBa_C0012
BBa_K389052	.	promoter	1401	1600	.	+	0	ID=BBa_R0010;Name=BBa_R0010;Parent=BBa_K389050
BBa_K389052	.	non_covalent_binding_site	1489	1526	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K389052	.	CDS	1401	1488	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K389052	.	non_covalent_binding_site	1566	1600	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K389052	.	promoter	1561	1566	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K389052	.	engineered_region	1401	1600	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K389052	.	promoter	1537	1542	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K389052	.	start_codon	1573	1573	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K389052	.	terminator	1225	1392	.	+	0	ID=BBa_B0017;Name=BBa_B0017;Parent=BBa_K389050
BBa_K389052	.	terminator	1313	1392	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0017
BBa_K389052	.	engineered_region	1313	1392	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K389052	.	stem_loop	1324	1367	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K389052	.	terminator	1225	1304	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0017
BBa_K389052	.	engineered_region	1225	1304	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K389052	.	stem_loop	1236	1279	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K389052	.	CDS	1627	2332	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_K389052	.	CDS	1627	2301	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K389052	.	engineered_tag	2308	2332	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
>BBa_K389052
tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatggtgaatgtgaaac
cagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagcca
cgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaa
ctggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcgg
cgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaa
agcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccatt
gctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattt
tctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcggg
cccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgata
gcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttccca
ctgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgc
ggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggat
tttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgt
tgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattc
attaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagct
taataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactg
ggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagccaggcatcaaataaaacgaaaggctcagtc
gaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagcaatacgcaaaccgcctctccc
cgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaatt
aatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtga
gcggataacaatttcacacatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttc
atgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgt
acgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgca
gttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggt
ttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtg
agttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttg
ggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagac
ggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccg
acatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactc
caccggtgcttaataacgctgatagtgctagtgtagatcgc