LOCUS       BBa_K389401             2887 bp    DNA     linear       26-Dec-2024
DEFINITION  Sensitivity tuner + luciferase
ACCESSION   BBa_K389401
VERSION     BBa_K389401.1  
FEATURES             Location/Qualifiers
     misc_feature    1..12
                     /label=B0034
     misc_feature    19..19
                     /label=P2 ogr
     CDS             19..237
                     /label=P2 ogr
     misc_feature    1..237
                     /label=BBa_I746350
     misc_feature    250..253
                     /label=conserved
     RBS             246..257
                     /label=BBa_B0034
     CDS             264..938
                     /label=mrfp1
     misc_feature    945..969
                     /label=Help:Barcodes
     CDS             264..969
                     /label=BBa_E1010
     stem_loop       989..1032
                     /label=stem_loop
     misc_feature    978..1057
                     /label=BBa_B0010
     terminator      978..1057
                     /label=BBa_B0010
     stem_loop       1073..1092
                     /label=T7 TE
     misc_feature    1099..1099
                     /label=stop
     polyA_site      1093..1106
                     /label=polya
     misc_feature    1066..1106
                     /label=BBa_B0012
     terminator      1066..1106
                     /label=BBa_B0012
     terminator      978..1106
                     /label=BBa_B0015
     promoter        1115..1205
                     /label=PF
     promoter        1115..1205
                     /label=BBa_I746360
     misc_feature    1218..1221
                     /label=conserved
     RBS             1214..1225
                     /label=BBa_B0034
     CDS             1232..2884
                     /label=Luciferase
     misc_feature    2885..2887
                     /label=Stop codon
     misc_feature    1232..2887
                     /label=BBa_K389004
ORIGIN
        1 aaagaggaga aatactagat gtttcattgt cctttatgcc agcatgccgc acatgcgcgt
       61 acaagtcgct atatcactga cacgacaaaa gagcgttatc atcagtgcca gaacgtgaat
      121 tgcagcgcca cgttcatcac ttatgagtcg gtacagcgat acatcgtgaa gccgggagaa
      181 gtccacgccg taaggccgca cccgttgcca tcagggcagc aaattatgtg gatgtaatac
      241 tagagaaaga ggagaaatac tagatggctt cctccgaaga cgttatcaaa gagttcatgc
      301 gtttcaaagt tcgtatggaa ggttccgtta acggtcacga gttcgaaatc gaaggtgaag
      361 gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact gaaagttacc aaaggtggtc
      421 cgctgccgtt cgcttgggac atcctgtccc cgcagttcca gtacggttcc aaagcttacg
      481 ttaaacaccc ggctgacatc ccggactacc tgaaactgtc cttcccggaa ggtttcaaat
      541 gggaacgtgt tatgaacttc gaagacggtg gtgttgttac cgttacccag gactcctccc
      601 tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg taccaacttc ccgtccgacg
      661 gtccggttat gcagaaaaaa accatgggtt gggaagcttc caccgaacgt atgtacccgg
      721 aagacggtgc tctgaaaggt gaaatcaaaa tgcgtctgaa actgaaagac ggtggtcact
      781 acgacgctga agttaaaacc acctacatgg ctaaaaaacc ggttcagctg ccgggtgctt
      841 acaaaaccga catcaaactg gacatcacct cccacaacga agactacacc atcgttgaac
      901 agtacgaacg tgctgaaggt cgtcactcca ccggtgctta ataacgctga tagtgctagt
      961 gtagatcgct actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     1021 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc
     1081 ttcgggtggg cctttctgcg tttatatact agagttgccc gccttttctt taccggtggt
     1141 tgtgctgtcg attagccaac cgggacaaat agcctgacat ctccggcgca actgaaaata
     1201 ccacttacta gagaaagagg agaaatacta gatggaagat gccaaaaaca ttaagaaggg
     1261 cccagcgcca ttctacccac tcgaagacgg gaccgccggc gagcagctgc acaaagccat
     1321 gaagcgctac gccctggtgc ccggcaccat cgcctttacc gacgcacata tcgaggtgga
     1381 cattacctac gccgagtact tcgagatgag cgttcggctg gcagaagcta tgaagcgcta
     1441 tgggctgaat acaaaccatc ggatcgtggt gtgcagcgag aatagcttgc agttcttcat
     1501 gcccgtgttg ggtgccctgt tcatcggtgt ggctgtggcc ccagctaacg acatctacaa
     1561 cgagcgcgag ctgctgaaca gcatgggcat cagccagccc accgtcgtat tcgtgagcaa
     1621 gaaagggctg caaaagatcc tcaacgtgca aaagaagcta ccgatcatac aaaagatcat
     1681 catcatggat agcaagaccg actaccaggg cttccaaagc atgtacacct tcgtgacttc
     1741 ccatttgcca cccggcttca acgagtacga cttcgtgccc gagagcttcg accgggacaa
     1801 aaccatcgcc ctgatcatga acagtagtgg cagtaccgga ttgcccaagg gcgtagccct
     1861 accgcaccgc accgcttgtg tccgattcag tcatgcccgc gaccccatct tcggcaacca
     1921 gatcatcccc gacaccgcta tcctcagcgt ggtgccattt caccacggct tcggcatgtt
     1981 caccacgctg ggctacttga tctgcggctt tcgggtcgtg ctcatgtacc gcttcgagga
     2041 ggagctattc ttgcgcagct tgcaagacta taagattcaa tctgccctgc tggtgcccac
     2101 actatttagc ttcttcgcta agagcactct catcgacaag tacgacctaa gcaacttgca
     2161 cgagatcgcc agcggcgggg cgccgctcag caaggaggta ggtgaggccg tggccaaacg
     2221 cttccaccta ccaggcatcc gccagggcta cggcctgaca gaaacaacca gcgccattct
     2281 gatcaccccc gaaggggacg acaagcctgg cgcagtaggc aaggtggtgc ccttcttcga
     2341 ggctaaggtg gtggacttgg acaccggtaa gacactgggt gtgaaccagc gcggcgagct
     2401 gtgcgtccgt ggccccatga tcatgagcgg ctacgttaac aaccccgagg ctacaaacgc
     2461 tctcatcgac aaggacggct ggctgcacag cggcgacatc gcctactggg acgaggacga
     2521 gcacttcttc atcgtggacc ggctgaagag cctgatcaaa tacaagggct accaggtagc
     2581 cccagccgaa ctggagagca tcctgctgca acaccccaac atcttcgacg ccggggtcgc
     2641 cggcctgccc gacgacgatg ccggcgagct gcccgccgca gtcgtcgtgc tggaacacgg
     2701 taaaaccatg accgagaagg agatcgtgga ctatgtggcc agccaggtta caaccgccaa
     2761 gaagctgcgc ggtggtgttg tgttcgtgga cgaggtgcct aaaggactga ccggcaagtt
     2821 ggacgcccgc aagatccgcg agattctcat taaggccaag aagggcggca agatcgccgt
     2881 gtaataa
//