##gff-version 3 ##sequence-region BBa_K389401 1 2887 BBa_K389401 . engineered_region 1232 2887 . + 0 ID=BBa_K389004;Name=BBa_K389004 BBa_K389401 . stop_codon 2885 2887 . + 0 ID=annotation2078364;Name=Stop codon;Parent=BBa_K389004 BBa_K389401 . CDS 1232 2884 . + 0 ID=annotation2077057;Name=Luciferase;Parent=BBa_K389004 BBa_K389401 . CDS 264 969 . + 0 ID=BBa_E1010;Name=BBa_E1010 BBa_K389401 . CDS 264 938 . + 0 ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010 BBa_K389401 . engineered_tag 945 969 . + 0 ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010 BBa_K389401 . ribosome_entry_site 1214 1225 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_K389401 . conserved_region 1218 1221 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_K389401 . terminator 978 1106 . + 0 ID=BBa_B0015;Name=BBa_B0015 BBa_K389401 . terminator 978 1057 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015 BBa_K389401 . stem_loop 989 1032 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K389401 . engineered_region 978 1057 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K389401 . terminator 1066 1106 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015 BBa_K389401 . polyA_site 1093 1106 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K389401 . stem_loop 1073 1092 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K389401 . engineered_region 1066 1106 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K389401 . stop_codon 1099 1099 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K389401 . ribosome_entry_site 246 257 . + 0 ID=BBa_B0034;Name=BBa_B0034 BBa_K389401 . conserved_region 250 253 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_K389401 . promoter 1115 1205 . + 0 ID=BBa_I746360;Name=BBa_I746360 BBa_K389401 . promoter 1115 1205 . + 0 ID=annotation1943783;Name=PF;Parent=BBa_I746360 BBa_K389401 . engineered_region 1 237 . + 0 ID=BBa_I746350;Name=BBa_I746350 BBa_K389401 . engineered_region 1 12 . + 0 ID=annotation1943865;Name=B0034;Parent=BBa_I746350 BBa_K389401 . start_codon 19 19 . + 0 ID=annotation1943866;Name=P2 ogr;Parent=BBa_I746350 BBa_K389401 . CDS 19 237 . + 0 ID=annotation1943867;Name=P2 ogr;Parent=BBa_I746350 >BBa_K389401 aaagaggagaaatactagatgtttcattgtcctttatgccagcatgccgcacatgcgcgtacaagtcgctatatcactg acacgacaaaagagcgttatcatcagtgccagaacgtgaattgcagcgccacgttcatcacttatgagtcggtacagcg atacatcgtgaagccgggagaagtccacgccgtaaggccgcacccgttgccatcagggcagcaaattatgtggatgtaa tactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtat ggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgct aaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaag cttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttat gaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaa ctgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgta tgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctga agttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacc tcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgct gatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgt tttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata tactagagttgcccgccttttctttaccggtggttgtgctgtcgattagccaaccgggacaaatagcctgacatctccg gcgcaactgaaaataccacttactagagaaagaggagaaatactagatggaagatgccaaaaacattaagaagggccca gcgccattctacccactcgaagacgggaccgccggcgagcagctgcacaaagccatgaagcgctacgccctggtgcccg gcaccatcgcctttaccgacgcacatatcgaggtggacattacctacgccgagtacttcgagatgagcgttcggctggc agaagctatgaagcgctatgggctgaatacaaaccatcggatcgtggtgtgcagcgagaatagcttgcagttcttcatg cccgtgttgggtgccctgttcatcggtgtggctgtggccccagctaacgacatctacaacgagcgcgagctgctgaaca gcatgggcatcagccagcccaccgtcgtattcgtgagcaagaaagggctgcaaaagatcctcaacgtgcaaaagaagct accgatcatacaaaagatcatcatcatggatagcaagaccgactaccagggcttccaaagcatgtacaccttcgtgact tcccatttgccacccggcttcaacgagtacgacttcgtgcccgagagcttcgaccgggacaaaaccatcgccctgatca tgaacagtagtggcagtaccggattgcccaagggcgtagccctaccgcaccgcaccgcttgtgtccgattcagtcatgc ccgcgaccccatcttcggcaaccagatcatccccgacaccgctatcctcagcgtggtgccatttcaccacggcttcggc atgttcaccacgctgggctacttgatctgcggctttcgggtcgtgctcatgtaccgcttcgaggaggagctattcttgc gcagcttgcaagactataagattcaatctgccctgctggtgcccacactatttagcttcttcgctaagagcactctcat cgacaagtacgacctaagcaacttgcacgagatcgccagcggcggggcgccgctcagcaaggaggtaggtgaggccgtg gccaaacgcttccacctaccaggcatccgccagggctacggcctgacagaaacaaccagcgccattctgatcacccccg aaggggacgacaagcctggcgcagtaggcaaggtggtgcccttcttcgaggctaaggtggtggacttggacaccggtaa gacactgggtgtgaaccagcgcggcgagctgtgcgtccgtggccccatgatcatgagcggctacgttaacaaccccgag gctacaaacgctctcatcgacaaggacggctggctgcacagcggcgacatcgcctactgggacgaggacgagcacttct tcatcgtggaccggctgaagagcctgatcaaatacaagggctaccaggtagccccagccgaactggagagcatcctgct gcaacaccccaacatcttcgacgccggggtcgccggcctgcccgacgacgatgccggcgagctgcccgccgcagtcgtc gtgctggaacacggtaaaaccatgaccgagaaggagatcgtggactatgtggccagccaggttacaaccgccaagaagc tgcgcggtggtgttgtgttcgtggacgaggtgcctaaaggactgaccggcaagttggacgcccgcaagatccgcgagat tctcattaaggccaagaagggcggcaagatcgccgtgtaataa