LOCUS       BBa_K389403             2914 bp    DNA     linear       16-Feb-2025
DEFINITION  Sensitivity tuner + luciferase
ACCESSION   BBa_K389403
VERSION     BBa_K389403.1  
FEATURES             Location/Qualifiers
     misc_feature    1..12
                     /label=B0034
     CDS             22..264
                     /label=phiR73 delta
     misc_feature    22..22
                     /label=phiR73 delta
     misc_feature    102..102
                     /label=silent mutation to remove PstI site
     misc_feature    1..264
                     /label=BBa_I746352
     misc_feature    277..280
                     /label=conserved
     RBS             273..284
                     /label=BBa_B0034
     CDS             291..965
                     /label=mrfp1
     misc_feature    972..996
                     /label=Help:Barcodes
     CDS             291..996
                     /label=BBa_E1010
     stem_loop       1016..1059
                     /label=stem_loop
     misc_feature    1005..1084
                     /label=BBa_B0010
     terminator      1005..1084
                     /label=BBa_B0010
     stem_loop       1100..1119
                     /label=T7 TE
     misc_feature    1126..1126
                     /label=stop
     polyA_site      1120..1133
                     /label=polya
     misc_feature    1093..1133
                     /label=BBa_B0012
     terminator      1093..1133
                     /label=BBa_B0012
     terminator      1005..1133
                     /label=BBa_B0015
     promoter        1142..1232
                     /label=PF
     promoter        1142..1232
                     /label=BBa_I746360
     misc_feature    1245..1248
                     /label=conserved
     RBS             1241..1252
                     /label=BBa_B0034
     CDS             1259..2911
                     /label=Luciferase
     misc_feature    2912..2914
                     /label=Stop codon
     misc_feature    1259..2914
                     /label=BBa_K389004
ORIGIN
        1 aaagaggaga aatactagat gatgcgctgc cctttctgtc gtcattcagc gcatacccgc
       61 accagccggt atgtgagtga caatgtcaaa gaaagttatc tccagtgcca gaatatttac
      121 tgttcggcga catttaaaac gcatgagtca atttgtgccg tgattcgttc tccggtcacg
      181 gaggaaaaac cagcaccggc aagcacagca ccggctgttg tccgaaaagt taaaggctgt
      241 tacagctcac cattcaacca ttaatactag agaaagagga gaaatactag atggcttcct
      301 ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg tatggaaggt tccgttaacg
      361 gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc gtacgaaggt acccagaccg
      421 ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc ttgggacatc ctgtccccgc
      481 agttccagta cggttccaaa gcttacgtta aacacccggc tgacatcccg gactacctga
      541 aactgtcctt cccggaaggt ttcaaatggg aacgtgttat gaacttcgaa gacggtggtg
      601 ttgttaccgt tacccaggac tcctccctgc aagacggtga gttcatctac aaagttaaac
      661 tgcgtggtac caacttcccg tccgacggtc cggttatgca gaaaaaaacc atgggttggg
      721 aagcttccac cgaacgtatg tacccggaag acggtgctct gaaaggtgaa atcaaaatgc
      781 gtctgaaact gaaagacggt ggtcactacg acgctgaagt taaaaccacc tacatggcta
      841 aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat caaactggac atcacctccc
      901 acaacgaaga ctacaccatc gttgaacagt acgaacgtgc tgaaggtcgt cactccaccg
      961 gtgcttaata acgctgatag tgctagtgta gatcgctact agagccaggc atcaaataaa
     1021 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     1081 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga
     1141 gttgcccgcc ttttctttac cggtggttgt gctgtcgatt agccaaccgg gacaaatagc
     1201 ctgacatctc cggcgcaact gaaaatacca cttactagag aaagaggaga aatactagat
     1261 ggaagatgcc aaaaacatta agaagggccc agcgccattc tacccactcg aagacgggac
     1321 cgccggcgag cagctgcaca aagccatgaa gcgctacgcc ctggtgcccg gcaccatcgc
     1381 ctttaccgac gcacatatcg aggtggacat tacctacgcc gagtacttcg agatgagcgt
     1441 tcggctggca gaagctatga agcgctatgg gctgaataca aaccatcgga tcgtggtgtg
     1501 cagcgagaat agcttgcagt tcttcatgcc cgtgttgggt gccctgttca tcggtgtggc
     1561 tgtggcccca gctaacgaca tctacaacga gcgcgagctg ctgaacagca tgggcatcag
     1621 ccagcccacc gtcgtattcg tgagcaagaa agggctgcaa aagatcctca acgtgcaaaa
     1681 gaagctaccg atcatacaaa agatcatcat catggatagc aagaccgact accagggctt
     1741 ccaaagcatg tacaccttcg tgacttccca tttgccaccc ggcttcaacg agtacgactt
     1801 cgtgcccgag agcttcgacc gggacaaaac catcgccctg atcatgaaca gtagtggcag
     1861 taccggattg cccaagggcg tagccctacc gcaccgcacc gcttgtgtcc gattcagtca
     1921 tgcccgcgac cccatcttcg gcaaccagat catccccgac accgctatcc tcagcgtggt
     1981 gccatttcac cacggcttcg gcatgttcac cacgctgggc tacttgatct gcggctttcg
     2041 ggtcgtgctc atgtaccgct tcgaggagga gctattcttg cgcagcttgc aagactataa
     2101 gattcaatct gccctgctgg tgcccacact atttagcttc ttcgctaaga gcactctcat
     2161 cgacaagtac gacctaagca acttgcacga gatcgccagc ggcggggcgc cgctcagcaa
     2221 ggaggtaggt gaggccgtgg ccaaacgctt ccacctacca ggcatccgcc agggctacgg
     2281 cctgacagaa acaaccagcg ccattctgat cacccccgaa ggggacgaca agcctggcgc
     2341 agtaggcaag gtggtgccct tcttcgaggc taaggtggtg gacttggaca ccggtaagac
     2401 actgggtgtg aaccagcgcg gcgagctgtg cgtccgtggc cccatgatca tgagcggcta
     2461 cgttaacaac cccgaggcta caaacgctct catcgacaag gacggctggc tgcacagcgg
     2521 cgacatcgcc tactgggacg aggacgagca cttcttcatc gtggaccggc tgaagagcct
     2581 gatcaaatac aagggctacc aggtagcccc agccgaactg gagagcatcc tgctgcaaca
     2641 ccccaacatc ttcgacgccg gggtcgccgg cctgcccgac gacgatgccg gcgagctgcc
     2701 cgccgcagtc gtcgtgctgg aacacggtaa aaccatgacc gagaaggaga tcgtggacta
     2761 tgtggccagc caggttacaa ccgccaagaa gctgcgcggt ggtgttgtgt tcgtggacga
     2821 ggtgcctaaa ggactgaccg gcaagttgga cgcccgcaag atccgcgaga ttctcattaa
     2881 ggccaagaag ggcggcaaga tcgccgtgta ataa
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