##gff-version 3
##sequence-region BBa_K389403 1 2914
BBa_K389403	.	engineered_region	1259	2914	.	+	0	ID=BBa_K389004;Name=BBa_K389004
BBa_K389403	.	CDS	1259	2911	.	+	0	ID=annotation2077057;Name=Luciferase;Parent=BBa_K389004
BBa_K389403	.	stop_codon	2912	2914	.	+	0	ID=annotation2078364;Name=Stop codon;Parent=BBa_K389004
BBa_K389403	.	engineered_region	1	264	.	+	0	ID=BBa_I746352;Name=BBa_I746352
BBa_K389403	.	engineered_region	1	12	.	+	0	ID=annotation1943871;Name=B0034;Parent=BBa_I746352
BBa_K389403	.	start_codon	22	22	.	+	0	ID=annotation1943873;Name=phiR73 delta;Parent=BBa_I746352
BBa_K389403	.	CDS	22	264	.	+	0	ID=annotation1943872;Name=phiR73 delta;Parent=BBa_I746352
BBa_K389403	.	sequence_alteration	102	102	.	+	0	ID=annotation1943874;Name=silent mutation to remove PstI site;Parent=BBa_I746352
BBa_K389403	.	promoter	1142	1232	.	+	0	ID=BBa_I746360;Name=BBa_I746360
BBa_K389403	.	promoter	1142	1232	.	+	0	ID=annotation1943783;Name=PF;Parent=BBa_I746360
BBa_K389403	.	CDS	291	996	.	+	0	ID=BBa_E1010;Name=BBa_E1010
BBa_K389403	.	CDS	291	965	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K389403	.	engineered_tag	972	996	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K389403	.	terminator	1005	1133	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K389403	.	terminator	1093	1133	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K389403	.	polyA_site	1120	1133	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K389403	.	stop_codon	1126	1126	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K389403	.	stem_loop	1100	1119	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K389403	.	engineered_region	1093	1133	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K389403	.	terminator	1005	1084	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K389403	.	stem_loop	1016	1059	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K389403	.	engineered_region	1005	1084	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K389403	.	ribosome_entry_site	1241	1252	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K389403	.	conserved_region	1245	1248	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K389403	.	ribosome_entry_site	273	284	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K389403	.	conserved_region	277	280	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K389403
aaagaggagaaatactagatgatgcgctgccctttctgtcgtcattcagcgcatacccgcaccagccggtatgtgagtg
acaatgtcaaagaaagttatctccagtgccagaatatttactgttcggcgacatttaaaacgcatgagtcaatttgtgc
cgtgattcgttctccggtcacggaggaaaaaccagcaccggcaagcacagcaccggctgttgtccgaaaagttaaaggc
tgttacagctcaccattcaaccattaatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaa
agagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggt
cgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgt
ccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttccc
ggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaa
gacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaacca
tgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaact
gaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttac
aaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtc
gtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaag
gctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcac
cttcgggtgggcctttctgcgtttatatactagagttgcccgccttttctttaccggtggttgtgctgtcgattagcca
accgggacaaatagcctgacatctccggcgcaactgaaaataccacttactagagaaagaggagaaatactagatggaa
gatgccaaaaacattaagaagggcccagcgccattctacccactcgaagacgggaccgccggcgagcagctgcacaaag
ccatgaagcgctacgccctggtgcccggcaccatcgcctttaccgacgcacatatcgaggtggacattacctacgccga
gtacttcgagatgagcgttcggctggcagaagctatgaagcgctatgggctgaatacaaaccatcggatcgtggtgtgc
agcgagaatagcttgcagttcttcatgcccgtgttgggtgccctgttcatcggtgtggctgtggccccagctaacgaca
tctacaacgagcgcgagctgctgaacagcatgggcatcagccagcccaccgtcgtattcgtgagcaagaaagggctgca
aaagatcctcaacgtgcaaaagaagctaccgatcatacaaaagatcatcatcatggatagcaagaccgactaccagggc
ttccaaagcatgtacaccttcgtgacttcccatttgccacccggcttcaacgagtacgacttcgtgcccgagagcttcg
accgggacaaaaccatcgccctgatcatgaacagtagtggcagtaccggattgcccaagggcgtagccctaccgcaccg
caccgcttgtgtccgattcagtcatgcccgcgaccccatcttcggcaaccagatcatccccgacaccgctatcctcagc
gtggtgccatttcaccacggcttcggcatgttcaccacgctgggctacttgatctgcggctttcgggtcgtgctcatgt
accgcttcgaggaggagctattcttgcgcagcttgcaagactataagattcaatctgccctgctggtgcccacactatt
tagcttcttcgctaagagcactctcatcgacaagtacgacctaagcaacttgcacgagatcgccagcggcggggcgccg
ctcagcaaggaggtaggtgaggccgtggccaaacgcttccacctaccaggcatccgccagggctacggcctgacagaaa
caaccagcgccattctgatcacccccgaaggggacgacaagcctggcgcagtaggcaaggtggtgcccttcttcgaggc
taaggtggtggacttggacaccggtaagacactgggtgtgaaccagcgcggcgagctgtgcgtccgtggccccatgatc
atgagcggctacgttaacaaccccgaggctacaaacgctctcatcgacaaggacggctggctgcacagcggcgacatcg
cctactgggacgaggacgagcacttcttcatcgtggaccggctgaagagcctgatcaaatacaagggctaccaggtagc
cccagccgaactggagagcatcctgctgcaacaccccaacatcttcgacgccggggtcgccggcctgcccgacgacgat
gccggcgagctgcccgccgcagtcgtcgtgctggaacacggtaaaaccatgaccgagaaggagatcgtggactatgtgg
ccagccaggttacaaccgccaagaagctgcgcggtggtgttgtgttcgtggacgaggtgcctaaaggactgaccggcaa
gttggacgcccgcaagatccgcgagattctcattaaggccaagaagggcggcaagatcgccgtgtaataa