LOCUS BBa_K389411 4020 bp DNA linear 22-Nov-2024 DEFINITION vir promoter + sensitivity tuner + luciferase ACCESSION BBa_K389411 VERSION BBa_K389411.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23110 RBS 47..51 /label=RBS CDS 55..780 /label=virG misc_feature 781..783 /label=Stop codon CDS 44..783 /label=BBa_K389002 stem_loop 803..846 /label=stem_loop misc_feature 792..871 /label=BBa_B0010 terminator 792..871 /label=BBa_B0010 stem_loop 891..934 /label=stem_loop misc_feature 880..959 /label=BBa_B0010 terminator 880..959 /label=BBa_B0010 terminator 792..959 /label=BBa_B0017 misc_feature 1000..1011 /label=vir-box RBS 1120..1124 /label=rbs promoter 1056..1060 /label=-35 promoter 1080..1084 /label=-10 promoter 968..1125 /label=BBa_K389003 misc_feature 1134..1145 /label=B0034 misc_feature 1152..1152 /label=P2 ogr CDS 1152..1370 /label=P2 ogr misc_feature 1134..1370 /label=BBa_I746350 misc_feature 1383..1386 /label=conserved RBS 1379..1390 /label=BBa_B0034 CDS 1397..2071 /label=mrfp1 misc_feature 2078..2102 /label=Help:Barcodes CDS 1397..2102 /label=BBa_E1010 stem_loop 2122..2165 /label=stem_loop misc_feature 2111..2190 /label=BBa_B0010 terminator 2111..2190 /label=BBa_B0010 stem_loop 2206..2225 /label=T7 TE misc_feature 2232..2232 /label=stop polyA_site 2226..2239 /label=polya misc_feature 2199..2239 /label=BBa_B0012 terminator 2199..2239 /label=BBa_B0012 terminator 2111..2239 /label=BBa_B0015 promoter 2248..2338 /label=PF promoter 2248..2338 /label=BBa_I746360 misc_feature 2351..2354 /label=conserved RBS 2347..2358 /label=BBa_B0034 CDS 2365..4017 /label=Luciferase misc_feature 4018..4020 /label=Stop codon misc_feature 2365..4020 /label=BBa_K389004 misc_feature 1134..4020 /label=BBa_K389401 ORIGIN 1 tttacggcta gctcagtcct aggtacaatg ctagctacta gagaagagga gaaaatgaaa 61 cacgtcctgc tggttgatga tgatgtggct atgcgtcacc tgattatcga gtatctgacc 121 atccatgcct tcaaagtcac agccgttgcc gattccactc agtttacccg tgtactgtct 181 agtgctaccg tcgatgttgt cgtggtcgat ctgaatctgg ttcgtgaaga tggcctggaa 241 attgtccgta acctggcagc aaaaagcgat atccctatca ttgtgatcag cggggatcgt 301 ctggaggaaa cagacaaagt tgttgccctg gagctgggtg cttctgattt tattgccaaa 361 ccgttcagca tccgtgagtt tctggcccgt attcgtgtag ccctgcgcgt tcgtcctaac 421 gtcgttcgtt cgaaagaccg tcgttccttt tgttttaccg actggaccct gaatctgcgt 481 caacgtcgcc tgatgagcga agctggcggt gaagtgaaac tgacagccgg tgagttcaat 541 ctgctgctgg catttctgga aaaacctcgt gatgttctga gccgtgaaca gctgctgatt 601 gcctctcgtg tccgtgatga agaggtgtat gatcgttcta tcgatgtcct gattctgcgc 661 ctgcgtcgca aactggaagc cgatccgtct agtcctcagc tgattaaaac cgcccgtggt 721 gctgggtatt tcttcgatgc cgatgtccaa gtgtcacatg gtggcacgat ggctgcttaa 781 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 841 ttttatctgt tgtttgtcgg tgaacgctct ctactagagc caggcatcaa ataaaacgaa 901 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 961 actagaggca cccttaagtg catggaaagc cgttttcgct tcaaatgaaa tcgaaaagaa 1021 gaaaacaaaa atcctagagt aaccgaccct cccgataatc gtgaacatca gatgacagca 1081 tttcttccga ccgaagtggc tgtgttggtt atgagcttga ggagatacta gagaaagagg 1141 agaaatacta gatgtttcat tgtcctttat gccagcatgc cgcacatgcg cgtacaagtc 1201 gctatatcac tgacacgaca aaagagcgtt atcatcagtg ccagaacgtg aattgcagcg 1261 ccacgttcat cacttatgag tcggtacagc gatacatcgt gaagccggga gaagtccacg 1321 ccgtaaggcc gcacccgttg ccatcagggc agcaaattat gtggatgtaa tactagagaa 1381 agaggagaaa tactagatgg cttcctccga agacgttatc aaagagttca tgcgtttcaa 1441 agttcgtatg gaaggttccg ttaacggtca cgagttcgaa atcgaaggtg aaggtgaagg 1501 tcgtccgtac gaaggtaccc agaccgctaa actgaaagtt accaaaggtg gtccgctgcc 1561 gttcgcttgg gacatcctgt ccccgcagtt ccagtacggt tccaaagctt acgttaaaca 1621 cccggctgac atcccggact acctgaaact gtccttcccg gaaggtttca aatgggaacg 1681 tgttatgaac ttcgaagacg gtggtgttgt taccgttacc caggactcct ccctgcaaga 1741 cggtgagttc atctacaaag ttaaactgcg tggtaccaac ttcccgtccg acggtccggt 1801 tatgcagaaa aaaaccatgg gttgggaagc ttccaccgaa cgtatgtacc cggaagacgg 1861 tgctctgaaa ggtgaaatca aaatgcgtct gaaactgaaa gacggtggtc actacgacgc 1921 tgaagttaaa accacctaca tggctaaaaa accggttcag ctgccgggtg cttacaaaac 1981 cgacatcaaa ctggacatca cctcccacaa cgaagactac accatcgttg aacagtacga 2041 acgtgctgaa ggtcgtcact ccaccggtgc ttaataacgc tgatagtgct agtgtagatc 2101 gctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 2161 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 2221 gggcctttct gcgtttatat actagagttg cccgcctttt ctttaccggt ggttgtgctg 2281 tcgattagcc aaccgggaca aatagcctga catctccggc gcaactgaaa ataccactta 2341 ctagagaaag aggagaaata ctagatggaa gatgccaaaa acattaagaa gggcccagcg 2401 ccattctacc cactcgaaga cgggaccgcc ggcgagcagc tgcacaaagc catgaagcgc 2461 tacgccctgg tgcccggcac catcgccttt accgacgcac atatcgaggt ggacattacc 2521 tacgccgagt acttcgagat gagcgttcgg ctggcagaag ctatgaagcg ctatgggctg 2581 aatacaaacc atcggatcgt ggtgtgcagc gagaatagct tgcagttctt catgcccgtg 2641 ttgggtgccc tgttcatcgg tgtggctgtg gccccagcta acgacatcta caacgagcgc 2701 gagctgctga acagcatggg catcagccag cccaccgtcg tattcgtgag caagaaaggg 2761 ctgcaaaaga tcctcaacgt gcaaaagaag ctaccgatca tacaaaagat catcatcatg 2821 gatagcaaga ccgactacca gggcttccaa agcatgtaca ccttcgtgac ttcccatttg 2881 ccacccggct tcaacgagta cgacttcgtg cccgagagct tcgaccggga caaaaccatc 2941 gccctgatca tgaacagtag tggcagtacc ggattgccca agggcgtagc cctaccgcac 3001 cgcaccgctt gtgtccgatt cagtcatgcc cgcgacccca tcttcggcaa ccagatcatc 3061 cccgacaccg ctatcctcag cgtggtgcca tttcaccacg gcttcggcat gttcaccacg 3121 ctgggctact tgatctgcgg ctttcgggtc gtgctcatgt accgcttcga ggaggagcta 3181 ttcttgcgca gcttgcaaga ctataagatt caatctgccc tgctggtgcc cacactattt 3241 agcttcttcg ctaagagcac tctcatcgac aagtacgacc taagcaactt gcacgagatc 3301 gccagcggcg gggcgccgct cagcaaggag gtaggtgagg ccgtggccaa acgcttccac 3361 ctaccaggca tccgccaggg ctacggcctg acagaaacaa ccagcgccat tctgatcacc 3421 cccgaagggg acgacaagcc tggcgcagta ggcaaggtgg tgcccttctt cgaggctaag 3481 gtggtggact tggacaccgg taagacactg ggtgtgaacc agcgcggcga gctgtgcgtc 3541 cgtggcccca tgatcatgag cggctacgtt aacaaccccg aggctacaaa cgctctcatc 3601 gacaaggacg gctggctgca cagcggcgac atcgcctact gggacgagga cgagcacttc 3661 ttcatcgtgg accggctgaa gagcctgatc aaatacaagg gctaccaggt agccccagcc 3721 gaactggaga gcatcctgct gcaacacccc aacatcttcg acgccggggt cgccggcctg 3781 cccgacgacg atgccggcga gctgcccgcc gcagtcgtcg tgctggaaca cggtaaaacc 3841 atgaccgaga aggagatcgt ggactatgtg gccagccagg ttacaaccgc caagaagctg 3901 cgcggtggtg ttgtgttcgt ggacgaggtg cctaaaggac tgaccggcaa gttggacgcc 3961 cgcaagatcc gcgagattct cattaaggcc aagaagggcg gcaagatcgc cgtgtaataa //