##gff-version 3
##sequence-region BBa_K389412 1 4020
BBa_K389412	.	promoter	968	1125	.	+	0	ID=BBa_K389003;Name=BBa_K389003
BBa_K389412	.	promoter	1080	1084	.	+	0	ID=annotation2077851;Name=-10;Parent=BBa_K389003
BBa_K389412	.	promoter	1056	1060	.	+	0	ID=annotation2077850;Name=-35;Parent=BBa_K389003
BBa_K389412	.	ribosome_entry_site	1120	1124	.	+	0	ID=annotation2077849;Name=rbs;Parent=BBa_K389003
BBa_K389412	.	non_covalent_binding_site	1000	1011	.	+	0	ID=annotation2077848;Name=vir-box;Parent=BBa_K389003
BBa_K389412	.	CDS	44	783	.	+	0	ID=BBa_K389002;Name=BBa_K389002
BBa_K389412	.	stop_codon	781	783	.	+	0	ID=annotation2078362;Name=Stop codon;Parent=BBa_K389002
BBa_K389412	.	CDS	55	780	.	+	0	ID=annotation2078361;Name=virG;Parent=BBa_K389002
BBa_K389412	.	ribosome_entry_site	47	51	.	+	0	ID=annotation2078360;Name=RBS;Parent=BBa_K389002
BBa_K389412	.	promoter	1	35	.	+	0	ID=BBa_J23110;Name=BBa_J23110
BBa_K389412	.	engineered_region	1134	4020	.	+	0	ID=BBa_K389402;Name=BBa_K389402
BBa_K389412	.	promoter	2248	2338	.	+	0	ID=BBa_I746360;Name=BBa_I746360;Parent=BBa_K389402
BBa_K389412	.	promoter	2248	2338	.	+	0	ID=annotation1943783;Name=PF;Parent=BBa_I746360
BBa_K389412	.	engineered_region	1134	1370	.	+	0	ID=BBa_I746351;Name=BBa_I746351;Parent=BBa_K389402
BBa_K389412	.	start_codon	1152	1152	.	+	0	ID=annotation1943869;Name=PSP3 pag;Parent=BBa_I746351
BBa_K389412	.	engineered_region	1134	1145	.	+	0	ID=annotation1943868;Name=B0034;Parent=BBa_I746351
BBa_K389412	.	CDS	1152	1370	.	+	0	ID=annotation1943870;Name=PSP3 pag;Parent=BBa_I746351
BBa_K389412	.	engineered_region	2365	4020	.	+	0	ID=BBa_K389004;Name=BBa_K389004;Parent=BBa_K389402
BBa_K389412	.	CDS	2365	4017	.	+	0	ID=annotation2077057;Name=Luciferase;Parent=BBa_K389004
BBa_K389412	.	stop_codon	4018	4020	.	+	0	ID=annotation2078364;Name=Stop codon;Parent=BBa_K389004
BBa_K389412	.	CDS	1397	2102	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K389402
BBa_K389412	.	engineered_tag	2078	2102	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K389412	.	CDS	1397	2071	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K389412	.	ribosome_entry_site	1379	1390	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K389402
BBa_K389412	.	conserved_region	1383	1386	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K389412	.	ribosome_entry_site	2347	2358	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K389402
BBa_K389412	.	conserved_region	2351	2354	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K389412	.	terminator	2111	2239	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K389402
BBa_K389412	.	terminator	2199	2239	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K389412	.	polyA_site	2226	2239	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K389412	.	engineered_region	2199	2239	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K389412	.	stem_loop	2206	2225	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K389412	.	stop_codon	2232	2232	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K389412	.	terminator	2111	2190	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K389412	.	engineered_region	2111	2190	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K389412	.	stem_loop	2122	2165	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K389412	.	terminator	792	959	.	+	0	ID=BBa_B0017;Name=BBa_B0017
BBa_K389412	.	terminator	880	959	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0017
BBa_K389412	.	engineered_region	880	959	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K389412	.	stem_loop	891	934	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K389412	.	terminator	792	871	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0017
BBa_K389412	.	engineered_region	792	871	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K389412	.	stem_loop	803	846	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_K389412
tttacggctagctcagtcctaggtacaatgctagctactagagaagaggagaaaatgaaacacgtcctgctggttgatg
atgatgtggctatgcgtcacctgattatcgagtatctgaccatccatgccttcaaagtcacagccgttgccgattccac
tcagtttacccgtgtactgtctagtgctaccgtcgatgttgtcgtggtcgatctgaatctggttcgtgaagatggcctg
gaaattgtccgtaacctggcagcaaaaagcgatatccctatcattgtgatcagcggggatcgtctggaggaaacagaca
aagttgttgccctggagctgggtgcttctgattttattgccaaaccgttcagcatccgtgagtttctggcccgtattcg
tgtagccctgcgcgttcgtcctaacgtcgttcgttcgaaagaccgtcgttccttttgttttaccgactggaccctgaat
ctgcgtcaacgtcgcctgatgagcgaagctggcggtgaagtgaaactgacagccggtgagttcaatctgctgctggcat
ttctggaaaaacctcgtgatgttctgagccgtgaacagctgctgattgcctctcgtgtccgtgatgaagaggtgtatga
tcgttctatcgatgtcctgattctgcgcctgcgtcgcaaactggaagccgatccgtctagtcctcagctgattaaaacc
gcccgtggtgctgggtatttcttcgatgccgatgtccaagtgtcacatggtggcacgatggctgcttaataatactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcgg
tgaacgctctctactagaggcacccttaagtgcatggaaagccgttttcgcttcaaatgaaatcgaaaagaagaaaaca
aaaatcctagagtaaccgaccctcccgataatcgtgaacatcagatgacagcatttcttccgaccgaagtggctgtgtt
ggttatgagcttgaggagatactagagaaagaggagaaatactagatgatgcactgcccgttatgccaaaacgctgcac
atgctcgcactagccggtaccttagcaccgaaacgaaagaacgttatcaccagtgccaaaacataaattgcggatgtac
atttatcacttttgagacactatcaagattcattgtgaaaccggggactgttgatcctgctccgccccaccccatcaga
aaccaacaacagcaactttggctttgatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaa
agagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggt
cgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgt
ccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttccc
ggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaa
gacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaacca
tgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaact
gaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttac
aaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtc
gtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaag
gctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcac
cttcgggtgggcctttctgcgtttatatactagagttgcccgccttttctttaccggtggttgtgctgtcgattagcca
accgggacaaatagcctgacatctccggcgcaactgaaaataccacttactagagaaagaggagaaatactagatggaa
gatgccaaaaacattaagaagggcccagcgccattctacccactcgaagacgggaccgccggcgagcagctgcacaaag
ccatgaagcgctacgccctggtgcccggcaccatcgcctttaccgacgcacatatcgaggtggacattacctacgccga
gtacttcgagatgagcgttcggctggcagaagctatgaagcgctatgggctgaatacaaaccatcggatcgtggtgtgc
agcgagaatagcttgcagttcttcatgcccgtgttgggtgccctgttcatcggtgtggctgtggccccagctaacgaca
tctacaacgagcgcgagctgctgaacagcatgggcatcagccagcccaccgtcgtattcgtgagcaagaaagggctgca
aaagatcctcaacgtgcaaaagaagctaccgatcatacaaaagatcatcatcatggatagcaagaccgactaccagggc
ttccaaagcatgtacaccttcgtgacttcccatttgccacccggcttcaacgagtacgacttcgtgcccgagagcttcg
accgggacaaaaccatcgccctgatcatgaacagtagtggcagtaccggattgcccaagggcgtagccctaccgcaccg
caccgcttgtgtccgattcagtcatgcccgcgaccccatcttcggcaaccagatcatccccgacaccgctatcctcagc
gtggtgccatttcaccacggcttcggcatgttcaccacgctgggctacttgatctgcggctttcgggtcgtgctcatgt
accgcttcgaggaggagctattcttgcgcagcttgcaagactataagattcaatctgccctgctggtgcccacactatt
tagcttcttcgctaagagcactctcatcgacaagtacgacctaagcaacttgcacgagatcgccagcggcggggcgccg
ctcagcaaggaggtaggtgaggccgtggccaaacgcttccacctaccaggcatccgccagggctacggcctgacagaaa
caaccagcgccattctgatcacccccgaaggggacgacaagcctggcgcagtaggcaaggtggtgcccttcttcgaggc
taaggtggtggacttggacaccggtaagacactgggtgtgaaccagcgcggcgagctgtgcgtccgtggccccatgatc
atgagcggctacgttaacaaccccgaggctacaaacgctctcatcgacaaggacggctggctgcacagcggcgacatcg
cctactgggacgaggacgagcacttcttcatcgtggaccggctgaagagcctgatcaaatacaagggctaccaggtagc
cccagccgaactggagagcatcctgctgcaacaccccaacatcttcgacgccggggtcgccggcctgcccgacgacgat
gccggcgagctgcccgccgcagtcgtcgtgctggaacacggtaaaaccatgaccgagaaggagatcgtggactatgtgg
ccagccaggttacaaccgccaagaagctgcgcggtggtgttgtgttcgtggacgaggtgcctaaaggactgaccggcaa
gttggacgcccgcaagatccgcgagattctcattaaggccaagaagggcggcaagatcgccgtgtaataa