LOCUS BBa_K389413 4047 bp DNA linear 22-Nov-2024 DEFINITION vir promoter + sensitivity tuner + luciferase ACCESSION BBa_K389413 VERSION BBa_K389413.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23110 RBS 47..51 /label=RBS CDS 55..780 /label=virG misc_feature 781..783 /label=Stop codon CDS 44..783 /label=BBa_K389002 stem_loop 803..846 /label=stem_loop misc_feature 792..871 /label=BBa_B0010 terminator 792..871 /label=BBa_B0010 stem_loop 891..934 /label=stem_loop misc_feature 880..959 /label=BBa_B0010 terminator 880..959 /label=BBa_B0010 terminator 792..959 /label=BBa_B0017 misc_feature 1000..1011 /label=vir-box RBS 1120..1124 /label=rbs promoter 1056..1060 /label=-35 promoter 1080..1084 /label=-10 promoter 968..1125 /label=BBa_K389003 misc_feature 1134..1145 /label=B0034 CDS 1155..1397 /label=phiR73 delta misc_feature 1155..1155 /label=phiR73 delta misc_feature 1235..1235 /label=silent mutation to remove PstI site misc_feature 1134..1397 /label=BBa_I746352 misc_feature 1410..1413 /label=conserved RBS 1406..1417 /label=BBa_B0034 CDS 1424..2098 /label=mrfp1 misc_feature 2105..2129 /label=Help:Barcodes CDS 1424..2129 /label=BBa_E1010 stem_loop 2149..2192 /label=stem_loop misc_feature 2138..2217 /label=BBa_B0010 terminator 2138..2217 /label=BBa_B0010 stem_loop 2233..2252 /label=T7 TE misc_feature 2259..2259 /label=stop polyA_site 2253..2266 /label=polya misc_feature 2226..2266 /label=BBa_B0012 terminator 2226..2266 /label=BBa_B0012 terminator 2138..2266 /label=BBa_B0015 promoter 2275..2365 /label=PF promoter 2275..2365 /label=BBa_I746360 misc_feature 2378..2381 /label=conserved RBS 2374..2385 /label=BBa_B0034 CDS 2392..4044 /label=Luciferase misc_feature 4045..4047 /label=Stop codon misc_feature 2392..4047 /label=BBa_K389004 misc_feature 1134..4047 /label=BBa_K389403 ORIGIN 1 tttacggcta gctcagtcct aggtacaatg ctagctacta gagaagagga gaaaatgaaa 61 cacgtcctgc tggttgatga tgatgtggct atgcgtcacc tgattatcga gtatctgacc 121 atccatgcct tcaaagtcac agccgttgcc gattccactc agtttacccg tgtactgtct 181 agtgctaccg tcgatgttgt cgtggtcgat ctgaatctgg ttcgtgaaga tggcctggaa 241 attgtccgta acctggcagc aaaaagcgat atccctatca ttgtgatcag cggggatcgt 301 ctggaggaaa cagacaaagt tgttgccctg gagctgggtg cttctgattt tattgccaaa 361 ccgttcagca tccgtgagtt tctggcccgt attcgtgtag ccctgcgcgt tcgtcctaac 421 gtcgttcgtt cgaaagaccg tcgttccttt tgttttaccg actggaccct gaatctgcgt 481 caacgtcgcc tgatgagcga agctggcggt gaagtgaaac tgacagccgg tgagttcaat 541 ctgctgctgg catttctgga aaaacctcgt gatgttctga gccgtgaaca gctgctgatt 601 gcctctcgtg tccgtgatga agaggtgtat gatcgttcta tcgatgtcct gattctgcgc 661 ctgcgtcgca aactggaagc cgatccgtct agtcctcagc tgattaaaac cgcccgtggt 721 gctgggtatt tcttcgatgc cgatgtccaa gtgtcacatg gtggcacgat ggctgcttaa 781 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 841 ttttatctgt tgtttgtcgg tgaacgctct ctactagagc caggcatcaa ataaaacgaa 901 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 961 actagaggca cccttaagtg catggaaagc cgttttcgct tcaaatgaaa tcgaaaagaa 1021 gaaaacaaaa atcctagagt aaccgaccct cccgataatc gtgaacatca gatgacagca 1081 tttcttccga ccgaagtggc tgtgttggtt atgagcttga ggagatacta gagaaagagg 1141 agaaatacta gatgatgcgc tgccctttct gtcgtcattc agcgcatacc cgcaccagcc 1201 ggtatgtgag tgacaatgtc aaagaaagtt atctccagtg ccagaatatt tactgttcgg 1261 cgacatttaa aacgcatgag tcaatttgtg ccgtgattcg ttctccggtc acggaggaaa 1321 aaccagcacc ggcaagcaca gcaccggctg ttgtccgaaa agttaaaggc tgttacagct 1381 caccattcaa ccattaatac tagagaaaga ggagaaatac tagatggctt cctccgaaga 1441 cgttatcaaa gagttcatgc gtttcaaagt tcgtatggaa ggttccgtta acggtcacga 1501 gttcgaaatc gaaggtgaag gtgaaggtcg tccgtacgaa ggtacccaga ccgctaaact 1561 gaaagttacc aaaggtggtc cgctgccgtt cgcttgggac atcctgtccc cgcagttcca 1621 gtacggttcc aaagcttacg ttaaacaccc ggctgacatc ccggactacc tgaaactgtc 1681 cttcccggaa ggtttcaaat gggaacgtgt tatgaacttc gaagacggtg gtgttgttac 1741 cgttacccag gactcctccc tgcaagacgg tgagttcatc tacaaagtta aactgcgtgg 1801 taccaacttc ccgtccgacg gtccggttat gcagaaaaaa accatgggtt gggaagcttc 1861 caccgaacgt atgtacccgg aagacggtgc tctgaaaggt gaaatcaaaa tgcgtctgaa 1921 actgaaagac ggtggtcact acgacgctga agttaaaacc acctacatgg ctaaaaaacc 1981 ggttcagctg ccgggtgctt acaaaaccga catcaaactg gacatcacct cccacaacga 2041 agactacacc atcgttgaac agtacgaacg tgctgaaggt cgtcactcca ccggtgctta 2101 ataacgctga tagtgctagt gtagatcgct actagagcca ggcatcaaat aaaacgaaag 2161 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac 2221 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagttgccc 2281 gccttttctt taccggtggt tgtgctgtcg attagccaac cgggacaaat agcctgacat 2341 ctccggcgca actgaaaata ccacttacta gagaaagagg agaaatacta gatggaagat 2401 gccaaaaaca ttaagaaggg cccagcgcca ttctacccac tcgaagacgg gaccgccggc 2461 gagcagctgc acaaagccat gaagcgctac gccctggtgc ccggcaccat cgcctttacc 2521 gacgcacata tcgaggtgga cattacctac gccgagtact tcgagatgag cgttcggctg 2581 gcagaagcta tgaagcgcta tgggctgaat acaaaccatc ggatcgtggt gtgcagcgag 2641 aatagcttgc agttcttcat gcccgtgttg ggtgccctgt tcatcggtgt ggctgtggcc 2701 ccagctaacg acatctacaa cgagcgcgag ctgctgaaca gcatgggcat cagccagccc 2761 accgtcgtat tcgtgagcaa gaaagggctg caaaagatcc tcaacgtgca aaagaagcta 2821 ccgatcatac aaaagatcat catcatggat agcaagaccg actaccaggg cttccaaagc 2881 atgtacacct tcgtgacttc ccatttgcca cccggcttca acgagtacga cttcgtgccc 2941 gagagcttcg accgggacaa aaccatcgcc ctgatcatga acagtagtgg cagtaccgga 3001 ttgcccaagg gcgtagccct accgcaccgc accgcttgtg tccgattcag tcatgcccgc 3061 gaccccatct tcggcaacca gatcatcccc gacaccgcta tcctcagcgt ggtgccattt 3121 caccacggct tcggcatgtt caccacgctg ggctacttga tctgcggctt tcgggtcgtg 3181 ctcatgtacc gcttcgagga ggagctattc ttgcgcagct tgcaagacta taagattcaa 3241 tctgccctgc tggtgcccac actatttagc ttcttcgcta agagcactct catcgacaag 3301 tacgacctaa gcaacttgca cgagatcgcc agcggcgggg cgccgctcag caaggaggta 3361 ggtgaggccg tggccaaacg cttccaccta ccaggcatcc gccagggcta cggcctgaca 3421 gaaacaacca gcgccattct gatcaccccc gaaggggacg acaagcctgg cgcagtaggc 3481 aaggtggtgc ccttcttcga ggctaaggtg gtggacttgg acaccggtaa gacactgggt 3541 gtgaaccagc gcggcgagct gtgcgtccgt ggccccatga tcatgagcgg ctacgttaac 3601 aaccccgagg ctacaaacgc tctcatcgac aaggacggct ggctgcacag cggcgacatc 3661 gcctactggg acgaggacga gcacttcttc atcgtggacc ggctgaagag cctgatcaaa 3721 tacaagggct accaggtagc cccagccgaa ctggagagca tcctgctgca acaccccaac 3781 atcttcgacg ccggggtcgc cggcctgccc gacgacgatg ccggcgagct gcccgccgca 3841 gtcgtcgtgc tggaacacgg taaaaccatg accgagaagg agatcgtgga ctatgtggcc 3901 agccaggtta caaccgccaa gaagctgcgc ggtggtgttg tgttcgtgga cgaggtgcct 3961 aaaggactga ccggcaagtt ggacgcccgc aagatccgcg agattctcat taaggccaag 4021 aagggcggca agatcgccgt gtaataa //