##gff-version 3
##sequence-region BBa_K389413 1 4047
BBa_K389413	.	promoter	968	1125	.	+	0	ID=BBa_K389003;Name=BBa_K389003
BBa_K389413	.	non_covalent_binding_site	1000	1011	.	+	0	ID=annotation2077848;Name=vir-box;Parent=BBa_K389003
BBa_K389413	.	ribosome_entry_site	1120	1124	.	+	0	ID=annotation2077849;Name=rbs;Parent=BBa_K389003
BBa_K389413	.	promoter	1056	1060	.	+	0	ID=annotation2077850;Name=-35;Parent=BBa_K389003
BBa_K389413	.	promoter	1080	1084	.	+	0	ID=annotation2077851;Name=-10;Parent=BBa_K389003
BBa_K389413	.	engineered_region	1134	4047	.	+	0	ID=BBa_K389403;Name=BBa_K389403
BBa_K389413	.	engineered_region	1134	1397	.	+	0	ID=BBa_I746352;Name=BBa_I746352;Parent=BBa_K389403
BBa_K389413	.	start_codon	1155	1155	.	+	0	ID=annotation1943873;Name=phiR73 delta;Parent=BBa_I746352
BBa_K389413	.	CDS	1155	1397	.	+	0	ID=annotation1943872;Name=phiR73 delta;Parent=BBa_I746352
BBa_K389413	.	engineered_region	1134	1145	.	+	0	ID=annotation1943871;Name=B0034;Parent=BBa_I746352
BBa_K389413	.	sequence_alteration	1235	1235	.	+	0	ID=annotation1943874;Name=silent mutation to remove PstI site;Parent=BBa_I746352
BBa_K389413	.	ribosome_entry_site	1406	1417	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K389403
BBa_K389413	.	conserved_region	1410	1413	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K389413	.	promoter	2275	2365	.	+	0	ID=BBa_I746360;Name=BBa_I746360;Parent=BBa_K389403
BBa_K389413	.	promoter	2275	2365	.	+	0	ID=annotation1943783;Name=PF;Parent=BBa_I746360
BBa_K389413	.	ribosome_entry_site	2374	2385	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K389403
BBa_K389413	.	conserved_region	2378	2381	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K389413	.	terminator	2138	2266	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K389403
BBa_K389413	.	terminator	2138	2217	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K389413	.	stem_loop	2149	2192	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K389413	.	engineered_region	2138	2217	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K389413	.	terminator	2226	2266	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K389413	.	polyA_site	2253	2266	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K389413	.	stem_loop	2233	2252	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K389413	.	engineered_region	2226	2266	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K389413	.	stop_codon	2259	2259	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K389413	.	engineered_region	2392	4047	.	+	0	ID=BBa_K389004;Name=BBa_K389004;Parent=BBa_K389403
BBa_K389413	.	stop_codon	4045	4047	.	+	0	ID=annotation2078364;Name=Stop codon;Parent=BBa_K389004
BBa_K389413	.	CDS	2392	4044	.	+	0	ID=annotation2077057;Name=Luciferase;Parent=BBa_K389004
BBa_K389413	.	CDS	1424	2129	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K389403
BBa_K389413	.	CDS	1424	2098	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K389413	.	engineered_tag	2105	2129	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K389413	.	promoter	1	35	.	+	0	ID=BBa_J23110;Name=BBa_J23110
BBa_K389413	.	CDS	44	783	.	+	0	ID=BBa_K389002;Name=BBa_K389002
BBa_K389413	.	ribosome_entry_site	47	51	.	+	0	ID=annotation2078360;Name=RBS;Parent=BBa_K389002
BBa_K389413	.	CDS	55	780	.	+	0	ID=annotation2078361;Name=virG;Parent=BBa_K389002
BBa_K389413	.	stop_codon	781	783	.	+	0	ID=annotation2078362;Name=Stop codon;Parent=BBa_K389002
BBa_K389413	.	terminator	792	959	.	+	0	ID=BBa_B0017;Name=BBa_B0017
BBa_K389413	.	terminator	792	871	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0017
BBa_K389413	.	stem_loop	803	846	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K389413	.	engineered_region	792	871	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K389413	.	terminator	880	959	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0017
BBa_K389413	.	stem_loop	891	934	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K389413	.	engineered_region	880	959	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_K389413
tttacggctagctcagtcctaggtacaatgctagctactagagaagaggagaaaatgaaacacgtcctgctggttgatg
atgatgtggctatgcgtcacctgattatcgagtatctgaccatccatgccttcaaagtcacagccgttgccgattccac
tcagtttacccgtgtactgtctagtgctaccgtcgatgttgtcgtggtcgatctgaatctggttcgtgaagatggcctg
gaaattgtccgtaacctggcagcaaaaagcgatatccctatcattgtgatcagcggggatcgtctggaggaaacagaca
aagttgttgccctggagctgggtgcttctgattttattgccaaaccgttcagcatccgtgagtttctggcccgtattcg
tgtagccctgcgcgttcgtcctaacgtcgttcgttcgaaagaccgtcgttccttttgttttaccgactggaccctgaat
ctgcgtcaacgtcgcctgatgagcgaagctggcggtgaagtgaaactgacagccggtgagttcaatctgctgctggcat
ttctggaaaaacctcgtgatgttctgagccgtgaacagctgctgattgcctctcgtgtccgtgatgaagaggtgtatga
tcgttctatcgatgtcctgattctgcgcctgcgtcgcaaactggaagccgatccgtctagtcctcagctgattaaaacc
gcccgtggtgctgggtatttcttcgatgccgatgtccaagtgtcacatggtggcacgatggctgcttaataatactaga
gccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctc
tctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcgg
tgaacgctctctactagaggcacccttaagtgcatggaaagccgttttcgcttcaaatgaaatcgaaaagaagaaaaca
aaaatcctagagtaaccgaccctcccgataatcgtgaacatcagatgacagcatttcttccgaccgaagtggctgtgtt
ggttatgagcttgaggagatactagagaaagaggagaaatactagatgatgcgctgccctttctgtcgtcattcagcgc
atacccgcaccagccggtatgtgagtgacaatgtcaaagaaagttatctccagtgccagaatatttactgttcggcgac
atttaaaacgcatgagtcaatttgtgccgtgattcgttctccggtcacggaggaaaaaccagcaccggcaagcacagca
ccggctgttgtccgaaaagttaaaggctgttacagctcaccattcaaccattaatactagagaaagaggagaaatacta
gatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgag
ttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtc
cgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacat
cccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgtt
accgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccg
acggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaa
aggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaa
aaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcg
ttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctact
agagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacg
ctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgcccgccttttcttt
accggtggttgtgctgtcgattagccaaccgggacaaatagcctgacatctccggcgcaactgaaaataccacttacta
gagaaagaggagaaatactagatggaagatgccaaaaacattaagaagggcccagcgccattctacccactcgaagacg
ggaccgccggcgagcagctgcacaaagccatgaagcgctacgccctggtgcccggcaccatcgcctttaccgacgcaca
tatcgaggtggacattacctacgccgagtacttcgagatgagcgttcggctggcagaagctatgaagcgctatgggctg
aatacaaaccatcggatcgtggtgtgcagcgagaatagcttgcagttcttcatgcccgtgttgggtgccctgttcatcg
gtgtggctgtggccccagctaacgacatctacaacgagcgcgagctgctgaacagcatgggcatcagccagcccaccgt
cgtattcgtgagcaagaaagggctgcaaaagatcctcaacgtgcaaaagaagctaccgatcatacaaaagatcatcatc
atggatagcaagaccgactaccagggcttccaaagcatgtacaccttcgtgacttcccatttgccacccggcttcaacg
agtacgacttcgtgcccgagagcttcgaccgggacaaaaccatcgccctgatcatgaacagtagtggcagtaccggatt
gcccaagggcgtagccctaccgcaccgcaccgcttgtgtccgattcagtcatgcccgcgaccccatcttcggcaaccag
atcatccccgacaccgctatcctcagcgtggtgccatttcaccacggcttcggcatgttcaccacgctgggctacttga
tctgcggctttcgggtcgtgctcatgtaccgcttcgaggaggagctattcttgcgcagcttgcaagactataagattca
atctgccctgctggtgcccacactatttagcttcttcgctaagagcactctcatcgacaagtacgacctaagcaacttg
cacgagatcgccagcggcggggcgccgctcagcaaggaggtaggtgaggccgtggccaaacgcttccacctaccaggca
tccgccagggctacggcctgacagaaacaaccagcgccattctgatcacccccgaaggggacgacaagcctggcgcagt
aggcaaggtggtgcccttcttcgaggctaaggtggtggacttggacaccggtaagacactgggtgtgaaccagcgcggc
gagctgtgcgtccgtggccccatgatcatgagcggctacgttaacaaccccgaggctacaaacgctctcatcgacaagg
acggctggctgcacagcggcgacatcgcctactgggacgaggacgagcacttcttcatcgtggaccggctgaagagcct
gatcaaatacaagggctaccaggtagccccagccgaactggagagcatcctgctgcaacaccccaacatcttcgacgcc
ggggtcgccggcctgcccgacgacgatgccggcgagctgcccgccgcagtcgtcgtgctggaacacggtaaaaccatga
ccgagaaggagatcgtggactatgtggccagccaggttacaaccgccaagaagctgcgcggtggtgttgtgttcgtgga
cgaggtgcctaaaggactgaccggcaagttggacgcccgcaagatccgcgagattctcattaaggccaagaagggcggc
aagatcgccgtgtaataa