BBa_K389413
type: ComponentDefinition
persistent identity: BBa K389413
display id: BBa_K389413
version: 1
was generated by: iGEM to SBOL conversion
description: vir promoter + sensitivity tuner + luciferase
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:19Z
mutable provenance: * luciferase from pGL4.10luc2 (Promega) * sensitivity tuner part, terminators and constitutive promoter from the partsregistry * ''virG'' was synthesized by Mr. Gene * ''vir'' promoter from Agrobacterium tumefaciens C58, TI-plasmid
owned by: james
owned by: myers
mutable description: This part combines a mutated virG response regulator from the VirA/G receptor system under the control of a constitutive promoter with a sensitivity tuner before a luciferase gene under the control of a vir promoter. The sensitivity tuner enhances a signal from a promoter so it enhances the readout luciferase.
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: sensitivity tuner to enhance the luciferase signal
star: false
creator: Jonas Aretz
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K389002
component: BBa_J23110
component: BBa_K389003
component: BBa_B0017
component: BBa_K389403
sequence annotation: BBa_J23110
sequence annotation: BBa_K389002
sequence annotation: BBa_B0017
sequence annotation: BBa_K389003
sequence annotation: BBa_K389403
sequence: https://synbiohub.org/public/igem/BBa_K389413_sequence/1

BBa_K389002
type: Component
persistent identity: component2300804
display id: component2300804
version: 1
top level: BBa_K389413
definition: virG
access: public

BBa_J23110
type: Component
persistent identity: component2300800
display id: component2300800
version: 1
top level: BBa_K389413
definition: BBa_J23110
access: public

BBa_K389003
type: Component
persistent identity: component2300814
display id: component2300814
version: 1
top level: BBa_K389413
definition: Pvir
access: public

BBa_B0017
type: Component
persistent identity: component2300809
display id: component2300809
version: 1
top level: BBa_K389413
definition: BBa_B0017
access: public

BBa_K389403
type: Component
persistent identity: component2300839
display id: component2300839
version: 1
top level: BBa_K389413
definition: BBa_K389403
access: public

BBa_J23110
type: SequenceAnnotation
persistent identity: annotation2300800
display id: annotation2300800
version: 1
top level: BBa_K389413
location: https://synbiohub.org/public/igem/BBa_K389413/annotation2300800/range2300800/1
component: BBa_J23110

https://synbiohub.org/public/igem/BBa_K389413/annotation2300800/range2300800/1
type: Range
persistent identity: range2300800
display id: range2300800
version: 1
top level: BBa_K389413
direction: direction/reverse
start: 1
end: 35
orientation: inline

BBa_K389002
type: SequenceAnnotation
persistent identity: annotation2300804
display id: annotation2300804
version: 1
top level: BBa_K389413
location: https://synbiohub.org/public/igem/BBa_K389413/annotation2300804/range2300804/1
component: BBa_K389002

https://synbiohub.org/public/igem/BBa_K389413/annotation2300804/range2300804/1
type: Range
persistent identity: range2300804
display id: range2300804
version: 1
top level: BBa_K389413
direction: direction/reverse
start: 44
end: 783
orientation: inline

BBa_B0017
type: SequenceAnnotation
persistent identity: annotation2300809
display id: annotation2300809
version: 1
top level: BBa_K389413
location: https://synbiohub.org/public/igem/BBa_K389413/annotation2300809/range2300809/1
component: BBa_B0017

https://synbiohub.org/public/igem/BBa_K389413/annotation2300809/range2300809/1
type: Range
persistent identity: range2300809
display id: range2300809
version: 1
top level: BBa_K389413
direction: direction/reverse
start: 792
end: 959
orientation: inline

BBa_K389003
type: SequenceAnnotation
persistent identity: annotation2300814
display id: annotation2300814
version: 1
top level: BBa_K389413
location: https://synbiohub.org/public/igem/BBa_K389413/annotation2300814/range2300814/1
component: BBa_K389003

https://synbiohub.org/public/igem/BBa_K389413/annotation2300814/range2300814/1
type: Range
persistent identity: range2300814
display id: range2300814
version: 1
top level: BBa_K389413
direction: direction/reverse
start: 968
end: 1125
orientation: inline

BBa_K389403
type: SequenceAnnotation
persistent identity: annotation2300839
display id: annotation2300839
version: 1
top level: BBa_K389413
location: https://synbiohub.org/public/igem/BBa_K389413/annotation2300839/range2300839/1
component: BBa_K389403

https://synbiohub.org/public/igem/BBa_K389413/annotation2300839/range2300839/1
type: Range
persistent identity: range2300839
display id: range2300839
version: 1
top level: BBa_K389413
direction: direction/reverse
start: 1134
end: 4047
orientation: inline

BBa_K389403
type: ComponentDefinition
persistent identity: BBa K389403
display id: BBa_K389403
version: 1
was generated by: iGEM to SBOL conversion
description: Sensitivity tuner + luciferase
created: 2010-10-10T11:00:00Z
modified: 2015-05-08T01:12:19Z
mutable provenance: * luciferase from pGL4.10luc2 (Promega) * the rest from the partsregistry
owned by: james
owned by: myers
mutable description: This is a modified <partinfo>BBa_I746390</partinfo> sensitivity tuner. Instead of a GFP reporter, this one has a firefly luciferase (from Promega's pGL4.10luc2 vector) as one reporter gene. The arabinose promoter has been removed from the original part so now it is possible to assemble this part behind any promoter to get an enhanced luciferase signal.
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: arabinose promoter removed, GFP exchanged with luciferase compared to the original part <partinfo>BBa_I746370</partinfo>
star: false
creator: Jonas Aretz
type: DnaRegion
role: SO:0000804
role: partType/Reporter
component: BBa_I746352
component: BBa_E1010
component: BBa_B0034
component: BBa_I746360
component: BBa_B0015
component: BBa_K389004
sequence annotation: BBa_I746352
sequence annotation: BBa_B0034
sequence annotation: BBa_E1010
sequence annotation: BBa_B0015
sequence annotation: BBa_I746360
sequence annotation: BBa_K389004
sequence: https://synbiohub.org/public/igem/BBa_K389403_sequence/1

BBa_I746352
type: Component
persistent identity: component2273101
display id: component2273101
version: 1
top level: BBa_K389403
definition: BBa_I746352
access: public

BBa_E1010
type: Component
persistent identity: component2273106
display id: component2273106
version: 1
top level: BBa_K389403
definition: mRFP1
access: public

BBa_B0034
type: Component
persistent identity: component2273103
display id: component2273103
version: 1
top level: BBa_K389403
definition: BBa_B0034
access: public

BBa_I746360
type: Component
persistent identity: component2273115
display id: component2273115
version: 1
top level: BBa_K389403
definition: BBa_I746360
access: public

BBa_B0015
type: Component
persistent identity: component2273113
display id: component2273113
version: 1
top level: BBa_K389403
definition: BBa_B0015
access: public

BBa_K389004
type: Component
persistent identity: component2273120
display id: component2273120
version: 1
top level: BBa_K389403
definition: luc
access: public

BBa_B0034
type: Component
persistent identity: component2273117
display id: component2273117
version: 1
top level: BBa_K389403
definition: BBa_B0034
access: public

BBa_I746352
type: SequenceAnnotation
persistent identity: annotation2273101
display id: annotation2273101
version: 1
top level: BBa_K389403
location: https://synbiohub.org/public/igem/BBa_K389403/annotation2273101/range2273101/1
component: BBa_I746352

https://synbiohub.org/public/igem/BBa_K389403/annotation2273101/range2273101/1
type: Range
persistent identity: range2273101
display id: range2273101
version: 1
top level: BBa_K389403
direction: direction/reverse
start: 1
end: 264
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2273103
display id: annotation2273103
version: 1
top level: BBa_K389403
location: https://synbiohub.org/public/igem/BBa_K389403/annotation2273103/range2273103/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K389403/annotation2273103/range2273103/1
type: Range
persistent identity: range2273103
display id: range2273103
version: 1
top level: BBa_K389403
direction: direction/reverse
start: 273
end: 284
orientation: inline

BBa_E1010
type: SequenceAnnotation
persistent identity: annotation2273106
display id: annotation2273106
version: 1
top level: BBa_K389403
location: https://synbiohub.org/public/igem/BBa_K389403/annotation2273106/range2273106/1
component: BBa_E1010

https://synbiohub.org/public/igem/BBa_K389403/annotation2273106/range2273106/1
type: Range
persistent identity: range2273106
display id: range2273106
version: 1
top level: BBa_K389403
direction: direction/reverse
start: 291
end: 996
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2273113
display id: annotation2273113
version: 1
top level: BBa_K389403
location: https://synbiohub.org/public/igem/BBa_K389403/annotation2273113/range2273113/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_K389403/annotation2273113/range2273113/1
type: Range
persistent identity: range2273113
display id: range2273113
version: 1
top level: BBa_K389403
direction: direction/reverse
start: 1005
end: 1133
orientation: inline

BBa_I746360
type: SequenceAnnotation
persistent identity: annotation2273115
display id: annotation2273115
version: 1
top level: BBa_K389403
location: https://synbiohub.org/public/igem/BBa_K389403/annotation2273115/range2273115/1
component: BBa_I746360

https://synbiohub.org/public/igem/BBa_K389403/annotation2273115/range2273115/1
type: Range
persistent identity: range2273115
display id: range2273115
version: 1
top level: BBa_K389403
direction: direction/reverse
start: 1142
end: 1232
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2273117
display id: annotation2273117
version: 1
top level: BBa_K389403
location: https://synbiohub.org/public/igem/BBa_K389403/annotation2273117/range2273117/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K389403/annotation2273117/range2273117/1
type: Range
persistent identity: range2273117
display id: range2273117
version: 1
top level: BBa_K389403
direction: direction/reverse
start: 1241
end: 1252
orientation: inline

BBa_K389004
type: SequenceAnnotation
persistent identity: annotation2273120
display id: annotation2273120
version: 1
top level: BBa_K389403
location: https://synbiohub.org/public/igem/BBa_K389403/annotation2273120/range2273120/1
component: BBa_K389004

https://synbiohub.org/public/igem/BBa_K389403/annotation2273120/range2273120/1
type: Range
persistent identity: range2273120
display id: range2273120
version: 1
top level: BBa_K389403
direction: direction/reverse
start: 1259
end: 2914
orientation: inline

BBa_J23110
type: ComponentDefinition
persistent identity: BBa J23110
display id: BBa_J23110
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Later
owned by: james
owned by: myers
mutable description: Later
discontinued: false
dominant: true
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23110_sequence/1

BBa_B0017
type: ComponentDefinition
persistent identity: BBa B0017
display id: BBa_B0017
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0010)
created: 2004-01-08T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator with two copies of <bb_part>BBa_B0010</bb_part>
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Austin Che
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0010
sequence annotation: BBa_B0010
sequence: https://synbiohub.org/public/igem/BBa_B0017_sequence/1

BBa_B0010
type: Component
persistent identity: component939331
display id: component939331
version: 1
top level: BBa_B0017
definition: BBa_B0010
access: public

BBa_B0010
type: Component
persistent identity: component939337
display id: component939337
version: 1
top level: BBa_B0017
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation939331
display id: annotation939331
version: 1
top level: BBa_B0017
location: https://synbiohub.org/public/igem/BBa_B0017/annotation939331/range939331/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0017/annotation939331/range939331/1
type: Range
persistent identity: range939331
display id: range939331
version: 1
top level: BBa_B0017
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation939337
display id: annotation939337
version: 1
top level: BBa_B0017
location: https://synbiohub.org/public/igem/BBa_B0017/annotation939337/range939337/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0017/annotation939337/range939337/1
type: Range
persistent identity: range939337
display id: range939337
version: 1
top level: BBa_B0017
direction: direction/forward
start: 89
end: 168
orientation: inline

virG
type: ComponentDefinition
persistent identity: BBa K389002
display id: BBa_K389002
version: 1
was generated by: iGEM to SBOL conversion
description: Mutated virG
created: 2010-08-09T11:00:00Z
modified: 2015-05-08T01:12:18Z
mutable provenance: synthetic
owned by: james
owned by: myers
mutable description: This is the ''virG'' response regulator from the virA/G receptor system. The virA/G receptor system is used by ''Agrobacterium tumefaciens'' to detect phenolic substances secreted by plants. In presence of these substances, ''virG'' is activated by the ''virA'' receptor and induces the transcription of genes under the control of a ''vir'' promotor. Normally, ''virG'' needs the ''rpoA'' RNA polymerase subunit from ''A. tumefaciens'' to work in ''Escherichia coli'' but this ''virG'' BioBrick is mutated, so works with the ''rpoA'' subunit from ''E. coli''.
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: synthesized by Mr. Gene, so optimated codon usage, removal of illegal restriction sites
star: false
creator: Jonas Aretz
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: RBS
sequence annotation: virG
sequence annotation: Stop codon
sequence: https://synbiohub.org/public/igem/BBa_K389002_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2078360
display id: annotation2078360
version: 1
top level: virG
location: https://synbiohub.org/public/igem/BBa_K389002/annotation2078360/range2078360/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K389002/annotation2078360/range2078360/1
type: Range
persistent identity: range2078360
display id: range2078360
version: 1
top level: virG
direction: direction/forward
start: 4
end: 8
orientation: inline

virG
type: SequenceAnnotation
persistent identity: annotation2078361
display id: annotation2078361
version: 1
top level: virG
location: https://synbiohub.org/public/igem/BBa_K389002/annotation2078361/range2078361/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K389002/annotation2078361/range2078361/1
type: Range
persistent identity: range2078361
display id: range2078361
version: 1
top level: virG
direction: direction/forward
start: 12
end: 737
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2078362
display id: annotation2078362
version: 1
top level: virG
location: https://synbiohub.org/public/igem/BBa_K389002/annotation2078362/range2078362/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K389002/annotation2078362/range2078362/1
type: Range
persistent identity: range2078362
display id: range2078362
version: 1
top level: virG
direction: direction/forward
start: 738
end: 740
orientation: inline

Pvir
type: ComponentDefinition
persistent identity: BBa K389003
display id: BBa_K389003
version: 1
was generated by: iGEM to SBOL conversion
description: virB promoter
created: 2010-08-09T11:00:00Z
modified: 2015-05-08T01:12:19Z
mutable provenance: ''A. tumefaciens'' C58, TI-plasmid
owned by: james
owned by: myers
mutable description: This is a ''vir'' promotor from ''Agrobacterium tumefaciens'' C58. It is located on the TI-plasmid before the ''virB'' genes. It is activated by phosphorylated ''virG'' response regulator.
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: contains already a RBS
star: false
creator: Jonas Aretz
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: vir-box
sequence annotation: -35
sequence annotation: -10
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_K389003_sequence/1

vir-box
type: SequenceAnnotation
persistent identity: annotation2077848
display id: annotation2077848
version: 1
top level: Pvir
location: https://synbiohub.org/public/igem/BBa_K389003/annotation2077848/range2077848/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_K389003/annotation2077848/range2077848/1
type: Range
persistent identity: range2077848
display id: range2077848
version: 1
top level: Pvir
direction: direction/forward
start: 33
end: 44
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2077850
display id: annotation2077850
version: 1
top level: Pvir
location: https://synbiohub.org/public/igem/BBa_K389003/annotation2077850/range2077850/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K389003/annotation2077850/range2077850/1
type: Range
persistent identity: range2077850
display id: range2077850
version: 1
top level: Pvir
direction: direction/forward
start: 89
end: 93
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2077851
display id: annotation2077851
version: 1
top level: Pvir
location: https://synbiohub.org/public/igem/BBa_K389003/annotation2077851/range2077851/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K389003/annotation2077851/range2077851/1
type: Range
persistent identity: range2077851
display id: range2077851
version: 1
top level: Pvir
direction: direction/forward
start: 113
end: 117
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation2077849
display id: annotation2077849
version: 1
top level: Pvir
location: https://synbiohub.org/public/igem/BBa_K389003/annotation2077849/range2077849/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K389003/annotation2077849/range2077849/1
type: Range
persistent identity: range2077849
display id: range2077849
version: 1
top level: Pvir
direction: direction/forward
start: 153
end: 157
orientation: inline

BBa_I746352
type: ComponentDefinition
persistent identity: BBa I746352
display id: BBa_I746352
version: 1
was generated by: iGEM to SBOL conversion
description: delta activator from phiR73 phage
created: 2007-09-11T11:00:00Z
modified: 2015-08-31T04:08:04Z
mutable provenance: plasmid DNA supplied by Prof. Richard Calendar, University of California.
owned by: james
owned by: myers
mutable description: The delta activator taken from phiR73 phage acts on a class of inducible promoters (parts I746360 to I746365), inducing their activity to varying degrees. The part sequence does already contain a ribosome binding site (B0034)!
discontinued: false
dominant: false
experience: experience/None
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: The part does contain a RBS (B0034) already.
star: true
creator: Stefan Milde
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: B0034
sequence annotation: phiR73 delta
sequence annotation: silent mutation to remove PstI site
sequence: https://synbiohub.org/public/igem/BBa_I746352_sequence/1

B0034
type: SequenceAnnotation
persistent identity: annotation1943871
display id: annotation1943871
version: 1
top level: BBa_I746352
location: https://synbiohub.org/public/igem/BBa_I746352/annotation1943871/range1943871/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_I746352/annotation1943871/range1943871/1
type: Range
persistent identity: range1943871
display id: range1943871
version: 1
top level: BBa_I746352
direction: direction/forward
start: 1
end: 12
orientation: inline

phiR73 delta
type: SequenceAnnotation
persistent identity: annotation1943872
display id: annotation1943872
version: 1
top level: BBa_I746352
location: https://synbiohub.org/public/igem/BBa_I746352/annotation1943872/range1943872/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I746352/annotation1943872/range1943872/1
type: Range
persistent identity: range1943872
display id: range1943872
version: 1
top level: BBa_I746352
direction: direction/forward
start: 22
end: 264
orientation: inline

phiR73 delta
type: SequenceAnnotation
persistent identity: annotation1943873
display id: annotation1943873
version: 1
top level: BBa_I746352
location: https://synbiohub.org/public/igem/BBa_I746352/annotation1943873/range1943873/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_I746352/annotation1943873/range1943873/1
type: Range
persistent identity: range1943873
display id: range1943873
version: 1
top level: BBa_I746352
direction: direction/forward
start: 22
end: 22
orientation: inline

silent mutation to remove PstI site
type: SequenceAnnotation
persistent identity: annotation1943874
display id: annotation1943874
version: 1
top level: BBa_I746352
location: https://synbiohub.org/public/igem/BBa_I746352/annotation1943874/range1943874/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_I746352/annotation1943874/range1943874/1
type: Range
persistent identity: range1943874
display id: range1943874
version: 1
top level: BBa_I746352
direction: direction/forward
start: 102
end: 102
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

mRFP1
type: ComponentDefinition
persistent identity: BBa E1010
display id: BBa_E1010
version: 1
was generated by: iGEM to SBOL conversion
description: **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral)
created: 2004-07-27T11:00:00Z
modified: 2015-08-31T04:07:26Z
mutable provenance: Campbell et al., PNAS v99 p7877 URL
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm
discontinued: false
dominant: false
experience: experience/Works
group u list: _11_1_
m user id: 0
owner id: 52
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A.
star: true
creator: Drew Endy
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: mrfp1
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_E1010_sequence/1

mrfp1
type: SequenceAnnotation
persistent identity: annotation1014044
display id: annotation1014044
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_E1010/annotation1014044/range1014044/1
type: Range
persistent identity: range1014044
display id: range1014044
version: 1
top level: mRFP1
direction: direction/forward
start: 1
end: 675
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214014
display id: annotation2214014
version: 1
top level: mRFP1
location: https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_E1010/annotation2214014/range2214014/1
type: Range
persistent identity: range2214014
display id: range2214014
version: 1
top level: mRFP1
direction: direction/forward
start: 682
end: 706
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_I746360
type: ComponentDefinition
persistent identity: BBa I746360
display id: BBa_I746360
version: 1
was generated by: iGEM to SBOL conversion
description: PF promoter from P2 phage
created: 2007-09-10T11:00:00Z
modified: 2015-08-31T04:08:04Z
mutable provenance: The original source is the P2 phage genome. For our work the DNA came from plasmids, kindly supplied to us by Prof. Calendar, University of California (Berkeley): Bacteriophage PSP3 and fR73 Activator Proteins: Analysis of Promoter Specificities; Julien and Calendar, 1996; JOURNAL OF BACTERIOLOGY, Oct. 1996, p. 5668???5675
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is the PF promoter taken from the P2 phage genome. It is an inducible promoter that is activated by a class of activators, including P2 ogr (I746350), PSP3 pag (I746351) and phiR73 delta (I746352). These different activators should cause different levels of activity of the PF promoter.
discontinued: false
dominant: false
experience: experience/None
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: no special considerations seemed necessary
star: true
creator: Stefan Milde
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: PF
sequence: https://synbiohub.org/public/igem/BBa_I746360_sequence/1

PF
type: SequenceAnnotation
persistent identity: annotation1943783
display id: annotation1943783
version: 1
top level: BBa_I746360
location: https://synbiohub.org/public/igem/BBa_I746360/annotation1943783/range1943783/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I746360/annotation1943783/range1943783/1
type: Range
persistent identity: range1943783
display id: range1943783
version: 1
top level: BBa_I746360
direction: direction/forward
start: 1
end: 91
orientation: inline

luc
type: ComponentDefinition
persistent identity: BBa K389004
display id: BBa_K389004
version: 1
was generated by: iGEM to SBOL conversion
description: Luciferase from pGL4.10[luc2]
created: 2010-08-09T11:00:00Z
modified: 2015-05-08T01:12:19Z
mutable provenance: pGL4.10luc2
owned by: james
owned by: myers
mutable description: A luciferase from Promega's pGL4.10luc2 vector.
discontinued: false
dominant: false
experience: experience/Works
group u list: _517_
m user id: 0
owner id: 6670
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: sequence of this vector is already published by Promega
star: false
creator: Jonas Aretz
type: DnaRegion
role: SO:0000804
role: partType/Reporter
sequence annotation: Luciferase
sequence annotation: Stop codon
sequence: https://synbiohub.org/public/igem/BBa_K389004_sequence/1

Luciferase
type: SequenceAnnotation
persistent identity: annotation2077057
display id: annotation2077057
version: 1
top level: luc
location: https://synbiohub.org/public/igem/BBa_K389004/annotation2077057/range2077057/1
role: SO:0000316
role: feature/protein

https://synbiohub.org/public/igem/BBa_K389004/annotation2077057/range2077057/1
type: Range
persistent identity: range2077057
display id: range2077057
version: 1
top level: luc
direction: direction/forward
start: 1
end: 1653
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2078364
display id: annotation2078364
version: 1
top level: luc
location: https://synbiohub.org/public/igem/BBa_K389004/annotation2078364/range2078364/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K389004/annotation2078364/range2078364/1
type: Range
persistent identity: range2078364
display id: range2078364
version: 1
top level: luc
direction: direction/forward
start: 1654
end: 1656
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

https://synbiohub.org/public/igem/BBa_K389413_sequence/1
type: Sequence
persistent identity: BBa K389413 sequence
display id: BBa_K389413_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtacaatgctagctactagagaagaggagaaaatgaaacacgtcctgctggttgatgatgatgtggctatgcgtcacctgattatcgagtatctgaccatccatgccttcaaagtcacagccgttgccgattccactcagtttacccgtgtactgtctagtgctaccgtcgatgttgtcgtggtcgatctgaatctggttcgtgaagatggcctggaaattgtccgtaacctggcagcaaaaagcgatatccctatcattgtgatcagcggggatcgtctggaggaaacagacaaagttgttgccctggagctgggtgcttctgattttattgccaaaccgttcagcatccgtgagtttctggcccgtattcgtgtagccctgcgcgttcgtcctaacgtcgttcgttcgaaagaccgtcgttccttttgttttaccgactggaccctgaatctgcgtcaacgtcgcctgatgagcgaagctggcggtgaagtgaaactgacagccggtgagttcaatctgctgctggcatttctggaaaaacctcgtgatgttctgagccgtgaacagctgctgattgcctctcgtgtccgtgatgaagaggtgtatgatcgttctatcgatgtcctgattctgcgcctgcgtcgcaaactggaagccgatccgtctagtcctcagctgattaaaaccgcccgtggtgctgggtatttcttcgatgccgatgtccaagtgtcacatggtggcacgatggctgcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagaggcacccttaagtgcatggaaagccgttttcgcttcaaatgaaatcgaaaagaagaaaacaaaaatcctagagtaaccgaccctcccgataatcgtgaacatcagatgacagcatttcttccgaccgaagtggctgtgttggttatgagcttgaggagatactagagaaagaggagaaatactagatgatgcgctgccctttctgtcgtcattcagcgcatacccgcaccagccggtatgtgagtgacaatgtcaaagaaagttatctccagtgccagaatatttactgttcggcgacatttaaaacgcatgagtcaatttgtgccgtgattcgttctccggtcacggaggaaaaaccagcaccggcaagcacagcaccggctgttgtccgaaaagttaaaggctgttacagctcaccattcaaccattaatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgctt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K389403_sequence/1
type: Sequence
persistent identity: BBa K389403 sequence
display id: BBa_K389403_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgatgcgctgccctttctgtcgtcattcagcgcatacccgcaccagccggtatgtgagtgacaatgtcaaagaaagttatctccagtgccagaatatttactgttcggcgacatttaaaacgcatgagtcaatttgtgccgtgattcgttctccggtcacggaggaaaaaccagcaccggcaagcacagcaccggctgttgtccgaaaagttaaaggctgttacagctcaccattcaaccattaatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgcccgccttttctttaccggtggttgtgctgtcgattagccaaccgggacaaatagcctgacatctccggcgcaactgaaaataccacttactagagaaagaggagaaatactagatggaagatgccaaaaacattaagaagggcccagcgccattctacccactcgaagacgggaccgccggcgagcagctgcacaaagccatgaagcgctacgccctggtgcccggcaccatcgcctttaccgacgcacatatcgaggtggacattacctacgccgagtacttcgagatgagcgttcggctggcagaagctatgaagcgctatgggctgaatacaaaccatcggatcgtggtgtgcagcgagaatagcttgcagttcttcatgcccgtgttgggtgccctgttcatcggtgtggctgtggccccagctaacgacatctacaacgagcgcgagctgctgaacagcatgggcatcagccagcccaccgtcgtattcgtgagcaagaaagggctgcaaaagatcctcaacgtgcaaaagaagctaccgatcatacaaaagatcatcatcatggatagcaagaccgactaccagggcttccaaagcatgtacaccttcgtgacttcccatttgccacccggcttcaacgagtacgacttcgtgcccgagagcttcgaccgggacaaaaccatcgccctgatcatgaacagtagtggcagtaccggattgcccaagggcgtagccctaccgcaccgcaccgcttgtgtccgattcagtcatgcccgcgaccccatcttcggcaaccagatcatccccgacaccgctatcctcagcgtggtgccatttcaccacggcttcg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23110_sequence/1
type: Sequence
persistent identity: BBa J23110 sequence
display id: BBa_J23110_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagtcctaggtacaatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0017_sequence/1
type: Sequence
persistent identity: BBa B0017 sequence
display id: BBa_B0017_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K389002_sequence/1
type: Sequence
persistent identity: BBa K389002 sequence
display id: BBa_K389002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aagaggagaaaatgaaacacgtcctgctggttgatgatgatgtggctatgcgtcacctgattatcgagtatctgaccatccatgccttcaaagtcacagccgttgccgattccactcagtttacccgtgtactgtctagtgctaccgtcgatgttgtcgtggtcgatctgaatctggttcgtgaagatggcctggaaattgtccgtaacctggcagcaaaaagcgatatccctatcattgtgatcagcggggatcgtctggaggaaacagacaaagttgttgccctggagctgggtgcttctgattttattgccaaaccgttcagcatccgtgagtttctggcccgtattcgtgtagccctgcgcgttcgtcctaacgtcgttcgttcgaaagaccgtcgttccttttgttttaccgactggaccctgaatctgcgtcaacgtcgcctgatgagcgaagctggcggtgaagtgaaactgacagccggtgagttcaatctgctgctggcatttctggaaaaacctcgtgatgttctgagccgtgaacagctgctgattgcctctcgtgtccgtgatgaagaggtgtatgatcgttctatcgatgtcctgattctgcgcctgcgtcgcaaactggaagccgatccgtctagtcctcagctgattaaaaccgcccgtggtgctgggtatttcttcgatgccgatgtccaagtgtcacatggtggcacgatggctgcttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K389003_sequence/1
type: Sequence
persistent identity: BBa K389003 sequence
display id: BBa_K389003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcacccttaagtgcatggaaagccgttttcgcttcaaatgaaatcgaaaagaagaaaacaaaaatcctagagtaaccgaccctcccgataatcgtgaacatcagatgacagcatttcttccgaccgaagtggctgtgttggttatgagcttgaggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I746352_sequence/1
type: Sequence
persistent identity: BBa I746352 sequence
display id: BBa_I746352_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgatgcgctgccctttctgtcgtcattcagcgcatacccgcaccagccggtatgtgagtgacaatgtcaaagaaagttatctccagtgccagaatatttactgttcggcgacatttaaaacgcatgagtcaatttgtgccgtgattcgttctccggtcacggaggaaaaaccagcaccggcaagcacagcaccggctgttgtccgaaaagttaaaggctgttacagctcaccattcaaccattaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E1010_sequence/1
type: Sequence
persistent identity: BBa E1010 sequence
display id: BBa_E1010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I746360_sequence/1
type: Sequence
persistent identity: BBa I746360 sequence
display id: BBa_I746360_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgcccgccttttctttaccggtggttgtgctgtcgattagccaaccgggacaaatagcctgacatctccggcgcaactgaaaataccact
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K389004_sequence/1
type: Sequence
persistent identity: BBa K389004 sequence
display id: BBa_K389004_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggaagatgccaaaaacattaagaagggcccagcgccattctacccactcgaagacgggaccgccggcgagcagctgcacaaagccatgaagcgctacgccctggtgcccggcaccatcgcctttaccgacgcacatatcgaggtggacattacctacgccgagtacttcgagatgagcgttcggctggcagaagctatgaagcgctatgggctgaatacaaaccatcggatcgtggtgtgcagcgagaatagcttgcagttcttcatgcccgtgttgggtgccctgttcatcggtgtggctgtggccccagctaacgacatctacaacgagcgcgagctgctgaacagcatgggcatcagccagcccaccgtcgtattcgtgagcaagaaagggctgcaaaagatcctcaacgtgcaaaagaagctaccgatcatacaaaagatcatcatcatggatagcaagaccgactaccagggcttccaaagcatgtacaccttcgtgacttcccatttgccacccggcttcaacgagtacgacttcgtgcccgagagcttcgaccgggacaaaaccatcgccctgatcatgaacagtagtggcagtaccggattgcccaagggcgtagccctaccgcaccgcaccgcttgtgtccgattcagtcatgcccgcgaccccatcttcggcaaccagatcatccccgacaccgctatcctcagcgtggtgccatttcaccacggcttcggcatgttcaccacgctgggctacttgatctgcggctttcgggtcgtgctcatgtaccgcttcgaggaggagctattcttgcgcagcttgcaagactataagattcaatctgccctgctggtgcccacactatttagcttcttcgctaagagcactctcatcgacaagtacgacctaagcaacttgcacgagatcgccagcggcggggcgccgctcagcaaggaggtaggtgaggccgtggccaaacgcttccacctaccaggcatccgccagggctacggcctgacagaaacaaccagcgccattctgatcacccccgaaggggacgacaagcctggcgcagtaggcaaggtggtgcccttcttcgaggctaaggtggtggacttggacaccggtaagacactgggtgtgaaccagcgcggcgagctgtgcgtccgtggccccatgatcatgagcggctacgttaacaaccccgaggctacaaacgctctcatcgacaaggacggctggctgcacagcggcgacatcgcctactgggacgaggacgagcacttcttcatcgtggaccggctgaagagcctgatcaaatacaagggctaccaggtagccccagccgaactggagagcatcctgctgcaacaccccaacatcttcgacgccggggtcgccggcctgcccgacgacgatgccggcgagctgcccgccgcagtcgtcgtgctggaacacggtaaaaccatgaccgagaaggagatcgtggactatgtggccagccaggttacaaccgccaagaagctgcgcggtggtgttgtgttcgtggacgaggtgcctaaaggactgaccggcaagttggacgcccgcaagatccgcgagattctcattaaggccaagaagggcggcaagatcgccgtgtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z