LOCUS BBa_K389421 6594 bp DNA linear 10-Jun-2024 DEFINITION VirA/G signaling system + sensitivity tuner + luciferase ACCESSION BBa_K389421 VERSION BBa_K389421.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23110 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 misc_feature 248..248 /label=Illegal PstI site removed CDS 62..2566 /label=virA misc_feature 2564..2566 /label=Stop codon CDS 62..2566 /label=BBa_K389001 misc_feature 1..2566 /label=BBa_K389010 promoter 2575..2609 /label=BBa_J23110 RBS 2621..2625 /label=RBS CDS 2629..3354 /label=virG misc_feature 3355..3357 /label=Stop codon CDS 2618..3357 /label=BBa_K389002 stem_loop 3377..3420 /label=stem_loop misc_feature 3366..3445 /label=BBa_B0010 terminator 3366..3445 /label=BBa_B0010 stem_loop 3465..3508 /label=stem_loop misc_feature 3454..3533 /label=BBa_B0010 terminator 3454..3533 /label=BBa_B0010 terminator 3366..3533 /label=BBa_B0017 misc_feature 3574..3585 /label=vir-box RBS 3694..3698 /label=rbs promoter 3630..3634 /label=-35 promoter 3654..3658 /label=-10 promoter 3542..3699 /label=BBa_K389003 promoter 1..3699 /label=BBa_K389017 misc_feature 3708..3719 /label=B0034 misc_feature 3726..3726 /label=P2 ogr CDS 3726..3944 /label=P2 ogr misc_feature 3708..3944 /label=BBa_I746350 misc_feature 3957..3960 /label=conserved RBS 3953..3964 /label=BBa_B0034 CDS 3971..4645 /label=mrfp1 misc_feature 4652..4676 /label=Help:Barcodes CDS 3971..4676 /label=BBa_E1010 stem_loop 4696..4739 /label=stem_loop misc_feature 4685..4764 /label=BBa_B0010 terminator 4685..4764 /label=BBa_B0010 stem_loop 4780..4799 /label=T7 TE misc_feature 4806..4806 /label=stop polyA_site 4800..4813 /label=polya misc_feature 4773..4813 /label=BBa_B0012 terminator 4773..4813 /label=BBa_B0012 terminator 4685..4813 /label=BBa_B0015 promoter 4822..4912 /label=PF promoter 4822..4912 /label=BBa_I746360 misc_feature 4925..4928 /label=conserved RBS 4921..4932 /label=BBa_B0034 CDS 4939..6591 /label=Luciferase misc_feature 6592..6594 /label=Stop codon misc_feature 4939..6594 /label=BBa_K389004 misc_feature 3708..6594 /label=BBa_K389401 ORIGIN 1 tttacggcta gctcagtcct aggtacaatg ctagctacta gagaaagagg agaaatacta 61 gatgaatgga aggtattcac cgtctcggca agatttcaag acaggcgcca agccttggtc 121 tatcctggcc ttggtagttg ctgcaatgat ttttgccttg atggcgatta cgtcttggca 181 ggacaatgaa accaatcggg cgatcctgac ccaattgcga gctattaaca tcgacagtgc 241 ttcgctccag cgggatgtac tccgcgcgga agcgggtgtg gtggcgaact accggcccat 301 tatctccagg ttgggagctt tgcggaagaa cctggaaaat ttgaagcgac tatttaaaca 361 atctcatctt gtgatcggca atgatttctc tcaactgctc gacaagctaa aggtgtctgt 421 ggatacgacc gacgcggccg ttgcagcctt cggagcgcaa aacgtgctcc tgcaagattc 481 gcttgccagc ttcactcgcg cgcttagtat tcttcccaaa atgtcgtcga cggatcagac 541 ggtcgaaaat tcgaacgaat tgggcagcct gatgctgcgg tttgtgcgtc agccaagccc 601 agcactctcg ttggagatca gtcacgaact cgacatgctc caaaaagcta gcggtggggc 661 tgaagttcct atccgtatac ttgcacgcga gggtcgcgtc atcttgtcga ttttgccccg 721 tgtgaacgat gccgtaaaca tgattcagac ctccgacacc gctgaaattg ccgaaagatt 781 ggagcgcaag tgtttggagg cctatagctt gcaaagcgtg agggagcagc gggcacggat 841 cttcttgggt tccgtttcgg tgggcctttg catctacatc atctcactgg tctataggct 901 gcgtcggaaa acggcttggt taacgcggcg tttggattac gaagaggtaa tcaaagagat 961 tggggtttgt ttcgaggggg gaggggccac agcgtcgtcc ctcaattcgt ccgcgcaagc 1021 tgcgcttgga attattcaac gcttctttaa tgcggaatcg tgtgcactag cattggtgga 1081 ccatggtgac aggtgggctg tcgaaagttt cgctgcgaag ctgcctgagc ccgtctggga 1141 ggacctcgcg ctacgcgaga tggtttctct tgccagagcg gatgagcgtg cgtcagtatt 1201 ccgcatcatg tcgacgcgaa aggtcagctg ccttcctccg gagactccgg gcgtttctat 1261 gctgctggca cacaaatcta cggatcaact gatagcgatt tgttccctcg gttaccaggg 1321 ctatcgtctg aaatcttgtc caggcgaagt tcagcttctt gaactcgcca ccgcctgcct 1381 ctgccactat atcgatgtcc ggcgtaagca gaccgaatgc gatattctgg agaggcgatt 1441 agagcatgcg gaacgtcttc aggcagttgg tacacttgct ggtggaatag cgcatgagtt 1501 caacaacatt ttgggagcaa tcctcgggta cgctgaaatg gcgcaaaaca tgctgcgtcg 1561 gtcatctgtc acccgaagac acattgacca aattatttcg tcgggtgaca gagccaggct 1621 cattatcgat cagatcttga ctctaagtcg aaaactagaa cgcgtgacaa agccgttcag 1681 tgtctccgaa ctcgtaatgg aaattgctcc cttattgcgt gttgcattgc agcgcaacat 1741 cgagctgaag ttcaagtttg acgacaagaa gagcgtagtc gaaggaagcc cgctagaggt 1801 tcagcagatg ctgatgaatc tttgcaagaa cgcttcccag gcgtttaccg ccgatggtca 1861 aatcgacatc atcgttagcc gaatttttgt atctcgacag aaagtactgg cgcatggtgt 1921 tatgccagct ggcgactatg ttcttctttc tgtcagcgac gacggtgaag gcattgccga 1981 aactgtgctc ccccacattt ttgagccttt ctttacaaca cgctcttgca gcggcggcac 2041 gggtctaggt cttgctgccg tgcacggtca tgtcagcgcg cttgcaggat atattgacgt 2101 tacttcagcc gtagggcgag ggacgcgctt cgacatttat ctacctcctt cctcgaagaa 2161 gcccgtcagc ccggacgcgt tttttgggcc ctgtaaaaca ccgcgtggca acggagaaat 2221 tgtggcattg attgagccag atcctgtctt gcgggaggtg tacgaagaca agatcgccgc 2281 tctgggctat gagccggtgg gctttaagac atgtgcagac ctttgcaatt ggatatcaaa 2341 aggcaagcaa gccgatctgg ttctagttga ccaatcgtct cttcccgaga atcagagtgc 2401 tactgctttg cacgcagcct tcaagacggc gtccatcatc attggaggaa gtgatcttaa 2461 aatgtcactt tccagcgatg acatgacgtc cgcgcttttt ctgcccaaac ctatatcctc 2521 caggacgatg gcctacgcga ttcgtaccaa gatcaaagcc taataatact agagtttacg 2581 gctagctcag tcctaggtac aatgctagct actagagaag aggagaaaat gaaacacgtc 2641 ctgctggttg atgatgatgt ggctatgcgt cacctgatta tcgagtatct gaccatccat 2701 gccttcaaag tcacagccgt tgccgattcc actcagttta cccgtgtact gtctagtgct 2761 accgtcgatg ttgtcgtggt cgatctgaat ctggttcgtg aagatggcct ggaaattgtc 2821 cgtaacctgg cagcaaaaag cgatatccct atcattgtga tcagcgggga tcgtctggag 2881 gaaacagaca aagttgttgc cctggagctg ggtgcttctg attttattgc caaaccgttc 2941 agcatccgtg agtttctggc ccgtattcgt gtagccctgc gcgttcgtcc taacgtcgtt 3001 cgttcgaaag accgtcgttc cttttgtttt accgactgga ccctgaatct gcgtcaacgt 3061 cgcctgatga gcgaagctgg cggtgaagtg aaactgacag ccggtgagtt caatctgctg 3121 ctggcatttc tggaaaaacc tcgtgatgtt ctgagccgtg aacagctgct gattgcctct 3181 cgtgtccgtg atgaagaggt gtatgatcgt tctatcgatg tcctgattct gcgcctgcgt 3241 cgcaaactgg aagccgatcc gtctagtcct cagctgatta aaaccgcccg tggtgctggg 3301 tatttcttcg atgccgatgt ccaagtgtca catggtggca cgatggctgc ttaataatac 3361 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 3421 ctgttgtttg tcggtgaacg ctctctacta gagccaggca tcaaataaaa cgaaaggctc 3481 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga 3541 ggcaccctta agtgcatgga aagccgtttt cgcttcaaat gaaatcgaaa agaagaaaac 3601 aaaaatccta gagtaaccga ccctcccgat aatcgtgaac atcagatgac agcatttctt 3661 ccgaccgaag tggctgtgtt ggttatgagc ttgaggagat actagagaaa gaggagaaat 3721 actagatgtt tcattgtcct ttatgccagc atgccgcaca tgcgcgtaca agtcgctata 3781 tcactgacac gacaaaagag cgttatcatc agtgccagaa cgtgaattgc agcgccacgt 3841 tcatcactta tgagtcggta cagcgataca tcgtgaagcc gggagaagtc cacgccgtaa 3901 ggccgcaccc gttgccatca gggcagcaaa ttatgtggat gtaatactag agaaagagga 3961 gaaatactag atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt tcaaagttcg 4021 tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg aaggtcgtcc 4081 gtacgaaggt acccagaccg ctaaactgaa agttaccaaa ggtggtccgc tgccgttcgc 4141 ttgggacatc ctgtccccgc agttccagta cggttccaaa gcttacgtta aacacccggc 4201 tgacatcccg gactacctga aactgtcctt cccggaaggt ttcaaatggg aacgtgttat 4261 gaacttcgaa gacggtggtg ttgttaccgt tacccaggac tcctccctgc aagacggtga 4321 gttcatctac aaagttaaac tgcgtggtac caacttcccg tccgacggtc cggttatgca 4381 gaaaaaaacc atgggttggg aagcttccac cgaacgtatg tacccggaag acggtgctct 4441 gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt ggtcactacg acgctgaagt 4501 taaaaccacc tacatggcta aaaaaccggt tcagctgccg ggtgcttaca aaaccgacat 4561 caaactggac atcacctccc acaacgaaga ctacaccatc gttgaacagt acgaacgtgc 4621 tgaaggtcgt cactccaccg gtgcttaata acgctgatag tgctagtgta gatcgctact 4681 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc 4741 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct 4801 ttctgcgttt atatactaga gttgcccgcc ttttctttac cggtggttgt gctgtcgatt 4861 agccaaccgg gacaaatagc ctgacatctc cggcgcaact gaaaatacca cttactagag 4921 aaagaggaga aatactagat ggaagatgcc aaaaacatta agaagggccc agcgccattc 4981 tacccactcg aagacgggac cgccggcgag cagctgcaca aagccatgaa gcgctacgcc 5041 ctggtgcccg gcaccatcgc ctttaccgac gcacatatcg aggtggacat tacctacgcc 5101 gagtacttcg agatgagcgt tcggctggca gaagctatga agcgctatgg gctgaataca 5161 aaccatcgga tcgtggtgtg cagcgagaat agcttgcagt tcttcatgcc cgtgttgggt 5221 gccctgttca tcggtgtggc tgtggcccca gctaacgaca tctacaacga gcgcgagctg 5281 ctgaacagca tgggcatcag ccagcccacc gtcgtattcg tgagcaagaa agggctgcaa 5341 aagatcctca acgtgcaaaa gaagctaccg atcatacaaa agatcatcat catggatagc 5401 aagaccgact accagggctt ccaaagcatg tacaccttcg tgacttccca tttgccaccc 5461 ggcttcaacg agtacgactt cgtgcccgag agcttcgacc gggacaaaac catcgccctg 5521 atcatgaaca gtagtggcag taccggattg cccaagggcg tagccctacc gcaccgcacc 5581 gcttgtgtcc gattcagtca tgcccgcgac cccatcttcg gcaaccagat catccccgac 5641 accgctatcc tcagcgtggt gccatttcac cacggcttcg gcatgttcac cacgctgggc 5701 tacttgatct gcggctttcg ggtcgtgctc atgtaccgct tcgaggagga gctattcttg 5761 cgcagcttgc aagactataa gattcaatct gccctgctgg tgcccacact atttagcttc 5821 ttcgctaaga gcactctcat cgacaagtac gacctaagca acttgcacga gatcgccagc 5881 ggcggggcgc cgctcagcaa ggaggtaggt gaggccgtgg ccaaacgctt ccacctacca 5941 ggcatccgcc agggctacgg cctgacagaa acaaccagcg ccattctgat cacccccgaa 6001 ggggacgaca agcctggcgc agtaggcaag gtggtgccct tcttcgaggc taaggtggtg 6061 gacttggaca ccggtaagac actgggtgtg aaccagcgcg gcgagctgtg cgtccgtggc 6121 cccatgatca tgagcggcta cgttaacaac cccgaggcta caaacgctct catcgacaag 6181 gacggctggc tgcacagcgg cgacatcgcc tactgggacg aggacgagca cttcttcatc 6241 gtggaccggc tgaagagcct gatcaaatac aagggctacc aggtagcccc agccgaactg 6301 gagagcatcc tgctgcaaca ccccaacatc ttcgacgccg gggtcgccgg cctgcccgac 6361 gacgatgccg gcgagctgcc cgccgcagtc gtcgtgctgg aacacggtaa aaccatgacc 6421 gagaaggaga tcgtggacta tgtggccagc caggttacaa ccgccaagaa gctgcgcggt 6481 ggtgttgtgt tcgtggacga ggtgcctaaa ggactgaccg gcaagttgga cgcccgcaag 6541 atccgcgaga ttctcattaa ggccaagaag ggcggcaaga tcgccgtgta ataa //