LOCUS BBa_K389423 6621 bp DNA linear 22-Nov-2024 DEFINITION VirA/G signaling system + sensitivity tuner + luciferase ACCESSION BBa_K389423 VERSION BBa_K389423.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23110 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 misc_feature 248..248 /label=Illegal PstI site removed CDS 62..2566 /label=virA misc_feature 2564..2566 /label=Stop codon CDS 62..2566 /label=BBa_K389001 misc_feature 1..2566 /label=BBa_K389010 promoter 2575..2609 /label=BBa_J23110 RBS 2621..2625 /label=RBS CDS 2629..3354 /label=virG misc_feature 3355..3357 /label=Stop codon CDS 2618..3357 /label=BBa_K389002 stem_loop 3377..3420 /label=stem_loop misc_feature 3366..3445 /label=BBa_B0010 terminator 3366..3445 /label=BBa_B0010 stem_loop 3465..3508 /label=stem_loop misc_feature 3454..3533 /label=BBa_B0010 terminator 3454..3533 /label=BBa_B0010 terminator 3366..3533 /label=BBa_B0017 misc_feature 3574..3585 /label=vir-box RBS 3694..3698 /label=rbs promoter 3630..3634 /label=-35 promoter 3654..3658 /label=-10 promoter 3542..3699 /label=BBa_K389003 promoter 1..3699 /label=BBa_K389017 misc_feature 3708..3719 /label=B0034 CDS 3729..3971 /label=phiR73 delta misc_feature 3729..3729 /label=phiR73 delta misc_feature 3809..3809 /label=silent mutation to remove PstI site misc_feature 3708..3971 /label=BBa_I746352 misc_feature 3984..3987 /label=conserved RBS 3980..3991 /label=BBa_B0034 CDS 3998..4672 /label=mrfp1 misc_feature 4679..4703 /label=Help:Barcodes CDS 3998..4703 /label=BBa_E1010 stem_loop 4723..4766 /label=stem_loop misc_feature 4712..4791 /label=BBa_B0010 terminator 4712..4791 /label=BBa_B0010 stem_loop 4807..4826 /label=T7 TE misc_feature 4833..4833 /label=stop polyA_site 4827..4840 /label=polya misc_feature 4800..4840 /label=BBa_B0012 terminator 4800..4840 /label=BBa_B0012 terminator 4712..4840 /label=BBa_B0015 promoter 4849..4939 /label=PF promoter 4849..4939 /label=BBa_I746360 misc_feature 4952..4955 /label=conserved RBS 4948..4959 /label=BBa_B0034 CDS 4966..6618 /label=Luciferase misc_feature 6619..6621 /label=Stop codon misc_feature 4966..6621 /label=BBa_K389004 misc_feature 3708..6621 /label=BBa_K389403 ORIGIN 1 tttacggcta gctcagtcct aggtacaatg ctagctacta gagaaagagg agaaatacta 61 gatgaatgga aggtattcac cgtctcggca agatttcaag acaggcgcca agccttggtc 121 tatcctggcc ttggtagttg ctgcaatgat ttttgccttg atggcgatta cgtcttggca 181 ggacaatgaa accaatcggg cgatcctgac ccaattgcga gctattaaca tcgacagtgc 241 ttcgctccag cgggatgtac tccgcgcgga agcgggtgtg gtggcgaact accggcccat 301 tatctccagg ttgggagctt tgcggaagaa cctggaaaat ttgaagcgac tatttaaaca 361 atctcatctt gtgatcggca atgatttctc tcaactgctc gacaagctaa aggtgtctgt 421 ggatacgacc gacgcggccg ttgcagcctt cggagcgcaa aacgtgctcc tgcaagattc 481 gcttgccagc ttcactcgcg cgcttagtat tcttcccaaa atgtcgtcga cggatcagac 541 ggtcgaaaat tcgaacgaat tgggcagcct gatgctgcgg tttgtgcgtc agccaagccc 601 agcactctcg ttggagatca gtcacgaact cgacatgctc caaaaagcta gcggtggggc 661 tgaagttcct atccgtatac ttgcacgcga gggtcgcgtc atcttgtcga ttttgccccg 721 tgtgaacgat gccgtaaaca tgattcagac ctccgacacc gctgaaattg ccgaaagatt 781 ggagcgcaag tgtttggagg cctatagctt gcaaagcgtg agggagcagc gggcacggat 841 cttcttgggt tccgtttcgg tgggcctttg catctacatc atctcactgg tctataggct 901 gcgtcggaaa acggcttggt taacgcggcg tttggattac gaagaggtaa tcaaagagat 961 tggggtttgt ttcgaggggg gaggggccac agcgtcgtcc ctcaattcgt ccgcgcaagc 1021 tgcgcttgga attattcaac gcttctttaa tgcggaatcg tgtgcactag cattggtgga 1081 ccatggtgac aggtgggctg tcgaaagttt cgctgcgaag ctgcctgagc ccgtctggga 1141 ggacctcgcg ctacgcgaga tggtttctct tgccagagcg gatgagcgtg cgtcagtatt 1201 ccgcatcatg tcgacgcgaa aggtcagctg ccttcctccg gagactccgg gcgtttctat 1261 gctgctggca cacaaatcta cggatcaact gatagcgatt tgttccctcg gttaccaggg 1321 ctatcgtctg aaatcttgtc caggcgaagt tcagcttctt gaactcgcca ccgcctgcct 1381 ctgccactat atcgatgtcc ggcgtaagca gaccgaatgc gatattctgg agaggcgatt 1441 agagcatgcg gaacgtcttc aggcagttgg tacacttgct ggtggaatag cgcatgagtt 1501 caacaacatt ttgggagcaa tcctcgggta cgctgaaatg gcgcaaaaca tgctgcgtcg 1561 gtcatctgtc acccgaagac acattgacca aattatttcg tcgggtgaca gagccaggct 1621 cattatcgat cagatcttga ctctaagtcg aaaactagaa cgcgtgacaa agccgttcag 1681 tgtctccgaa ctcgtaatgg aaattgctcc cttattgcgt gttgcattgc agcgcaacat 1741 cgagctgaag ttcaagtttg acgacaagaa gagcgtagtc gaaggaagcc cgctagaggt 1801 tcagcagatg ctgatgaatc tttgcaagaa cgcttcccag gcgtttaccg ccgatggtca 1861 aatcgacatc atcgttagcc gaatttttgt atctcgacag aaagtactgg cgcatggtgt 1921 tatgccagct ggcgactatg ttcttctttc tgtcagcgac gacggtgaag gcattgccga 1981 aactgtgctc ccccacattt ttgagccttt ctttacaaca cgctcttgca gcggcggcac 2041 gggtctaggt cttgctgccg tgcacggtca tgtcagcgcg cttgcaggat atattgacgt 2101 tacttcagcc gtagggcgag ggacgcgctt cgacatttat ctacctcctt cctcgaagaa 2161 gcccgtcagc ccggacgcgt tttttgggcc ctgtaaaaca ccgcgtggca acggagaaat 2221 tgtggcattg attgagccag atcctgtctt gcgggaggtg tacgaagaca agatcgccgc 2281 tctgggctat gagccggtgg gctttaagac atgtgcagac ctttgcaatt ggatatcaaa 2341 aggcaagcaa gccgatctgg ttctagttga ccaatcgtct cttcccgaga atcagagtgc 2401 tactgctttg cacgcagcct tcaagacggc gtccatcatc attggaggaa gtgatcttaa 2461 aatgtcactt tccagcgatg acatgacgtc cgcgcttttt ctgcccaaac ctatatcctc 2521 caggacgatg gcctacgcga ttcgtaccaa gatcaaagcc taataatact agagtttacg 2581 gctagctcag tcctaggtac aatgctagct actagagaag aggagaaaat gaaacacgtc 2641 ctgctggttg atgatgatgt ggctatgcgt cacctgatta tcgagtatct gaccatccat 2701 gccttcaaag tcacagccgt tgccgattcc actcagttta cccgtgtact gtctagtgct 2761 accgtcgatg ttgtcgtggt cgatctgaat ctggttcgtg aagatggcct ggaaattgtc 2821 cgtaacctgg cagcaaaaag cgatatccct atcattgtga tcagcgggga tcgtctggag 2881 gaaacagaca aagttgttgc cctggagctg ggtgcttctg attttattgc caaaccgttc 2941 agcatccgtg agtttctggc ccgtattcgt gtagccctgc gcgttcgtcc taacgtcgtt 3001 cgttcgaaag accgtcgttc cttttgtttt accgactgga ccctgaatct gcgtcaacgt 3061 cgcctgatga gcgaagctgg cggtgaagtg aaactgacag ccggtgagtt caatctgctg 3121 ctggcatttc tggaaaaacc tcgtgatgtt ctgagccgtg aacagctgct gattgcctct 3181 cgtgtccgtg atgaagaggt gtatgatcgt tctatcgatg tcctgattct gcgcctgcgt 3241 cgcaaactgg aagccgatcc gtctagtcct cagctgatta aaaccgcccg tggtgctggg 3301 tatttcttcg atgccgatgt ccaagtgtca catggtggca cgatggctgc ttaataatac 3361 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat 3421 ctgttgtttg tcggtgaacg ctctctacta gagccaggca tcaaataaaa cgaaaggctc 3481 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga 3541 ggcaccctta agtgcatgga aagccgtttt cgcttcaaat gaaatcgaaa agaagaaaac 3601 aaaaatccta gagtaaccga ccctcccgat aatcgtgaac atcagatgac agcatttctt 3661 ccgaccgaag tggctgtgtt ggttatgagc ttgaggagat actagagaaa gaggagaaat 3721 actagatgat gcgctgccct ttctgtcgtc attcagcgca tacccgcacc agccggtatg 3781 tgagtgacaa tgtcaaagaa agttatctcc agtgccagaa tatttactgt tcggcgacat 3841 ttaaaacgca tgagtcaatt tgtgccgtga ttcgttctcc ggtcacggag gaaaaaccag 3901 caccggcaag cacagcaccg gctgttgtcc gaaaagttaa aggctgttac agctcaccat 3961 tcaaccatta atactagaga aagaggagaa atactagatg gcttcctccg aagacgttat 4021 caaagagttc atgcgtttca aagttcgtat ggaaggttcc gttaacggtc acgagttcga 4081 aatcgaaggt gaaggtgaag gtcgtccgta cgaaggtacc cagaccgcta aactgaaagt 4141 taccaaaggt ggtccgctgc cgttcgcttg ggacatcctg tccccgcagt tccagtacgg 4201 ttccaaagct tacgttaaac acccggctga catcccggac tacctgaaac tgtccttccc 4261 ggaaggtttc aaatgggaac gtgttatgaa cttcgaagac ggtggtgttg ttaccgttac 4321 ccaggactcc tccctgcaag acggtgagtt catctacaaa gttaaactgc gtggtaccaa 4381 cttcccgtcc gacggtccgg ttatgcagaa aaaaaccatg ggttgggaag cttccaccga 4441 acgtatgtac ccggaagacg gtgctctgaa aggtgaaatc aaaatgcgtc tgaaactgaa 4501 agacggtggt cactacgacg ctgaagttaa aaccacctac atggctaaaa aaccggttca 4561 gctgccgggt gcttacaaaa ccgacatcaa actggacatc acctcccaca acgaagacta 4621 caccatcgtt gaacagtacg aacgtgctga aggtcgtcac tccaccggtg cttaataacg 4681 ctgatagtgc tagtgtagat cgctactaga gccaggcatc aaataaaacg aaaggctcag 4741 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt 4801 cacactggct caccttcggg tgggcctttc tgcgtttata tactagagtt gcccgccttt 4861 tctttaccgg tggttgtgct gtcgattagc caaccgggac aaatagcctg acatctccgg 4921 cgcaactgaa aataccactt actagagaaa gaggagaaat actagatgga agatgccaaa 4981 aacattaaga agggcccagc gccattctac ccactcgaag acgggaccgc cggcgagcag 5041 ctgcacaaag ccatgaagcg ctacgccctg gtgcccggca ccatcgcctt taccgacgca 5101 catatcgagg tggacattac ctacgccgag tacttcgaga tgagcgttcg gctggcagaa 5161 gctatgaagc gctatgggct gaatacaaac catcggatcg tggtgtgcag cgagaatagc 5221 ttgcagttct tcatgcccgt gttgggtgcc ctgttcatcg gtgtggctgt ggccccagct 5281 aacgacatct acaacgagcg cgagctgctg aacagcatgg gcatcagcca gcccaccgtc 5341 gtattcgtga gcaagaaagg gctgcaaaag atcctcaacg tgcaaaagaa gctaccgatc 5401 atacaaaaga tcatcatcat ggatagcaag accgactacc agggcttcca aagcatgtac 5461 accttcgtga cttcccattt gccacccggc ttcaacgagt acgacttcgt gcccgagagc 5521 ttcgaccggg acaaaaccat cgccctgatc atgaacagta gtggcagtac cggattgccc 5581 aagggcgtag ccctaccgca ccgcaccgct tgtgtccgat tcagtcatgc ccgcgacccc 5641 atcttcggca accagatcat ccccgacacc gctatcctca gcgtggtgcc atttcaccac 5701 ggcttcggca tgttcaccac gctgggctac ttgatctgcg gctttcgggt cgtgctcatg 5761 taccgcttcg aggaggagct attcttgcgc agcttgcaag actataagat tcaatctgcc 5821 ctgctggtgc ccacactatt tagcttcttc gctaagagca ctctcatcga caagtacgac 5881 ctaagcaact tgcacgagat cgccagcggc ggggcgccgc tcagcaagga ggtaggtgag 5941 gccgtggcca aacgcttcca cctaccaggc atccgccagg gctacggcct gacagaaaca 6001 accagcgcca ttctgatcac ccccgaaggg gacgacaagc ctggcgcagt aggcaaggtg 6061 gtgcccttct tcgaggctaa ggtggtggac ttggacaccg gtaagacact gggtgtgaac 6121 cagcgcggcg agctgtgcgt ccgtggcccc atgatcatga gcggctacgt taacaacccc 6181 gaggctacaa acgctctcat cgacaaggac ggctggctgc acagcggcga catcgcctac 6241 tgggacgagg acgagcactt cttcatcgtg gaccggctga agagcctgat caaatacaag 6301 ggctaccagg tagccccagc cgaactggag agcatcctgc tgcaacaccc caacatcttc 6361 gacgccgggg tcgccggcct gcccgacgac gatgccggcg agctgcccgc cgcagtcgtc 6421 gtgctggaac acggtaaaac catgaccgag aaggagatcg tggactatgt ggccagccag 6481 gttacaaccg ccaagaagct gcgcggtggt gttgtgttcg tggacgaggt gcctaaagga 6541 ctgaccggca agttggacgc ccgcaagatc cgcgagattc tcattaaggc caagaagggc 6601 ggcaagatcg ccgtgtaata a //