BBa_J23110 1 BBa_J23110 constitutive promoter family member 2006-08-16T11:00:00Z 2015-08-31T04:08:40Z Later Later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_K389017 1 VirA/G VirA/G signaling system without read-out 2010-09-27T11:00:00Z 2015-05-08T01:12:19Z ''A. tumefaciens'' C58 TI-plasmid, partsregistry, Mr. Gene This BioBrick is a virA/G signaling system without read-out. It contains an unmutated ''virA'' gene and a ''virG'' gene that works without ''rpoA'' from ''A. tumefaciens'' and a ''vir'' promoter. false false _517_ 0 6670 9 Not in stock false This BioBrick can be used to control any gene with a ''vir'' promoter false Jonas Aretz component2226697 1 BBa_J23110 component2226706 1 BBa_B0017 component2226696 1 BBa_K389010 component2226711 1 BBa_K389003 component2226701 1 BBa_K389002 annotation2226701 1 BBa_K389002 range2226701 1 2618 3357 annotation2226697 1 BBa_J23110 range2226697 1 2575 2609 annotation2226711 1 BBa_K389003 range2226711 1 3542 3699 annotation2226696 1 BBa_K389010 range2226696 1 1 2566 annotation2226706 1 BBa_B0017 range2226706 1 3366 3533 BBa_K389002 1 virG Mutated virG 2010-08-09T11:00:00Z 2015-05-08T01:12:18Z synthetic This is the ''virG'' response regulator from the virA/G receptor system. The virA/G receptor system is used by ''Agrobacterium tumefaciens'' to detect phenolic substances secreted by plants. In presence of these substances, ''virG'' is activated by the ''virA'' receptor and induces the transcription of genes under the control of a ''vir'' promotor. Normally, ''virG'' needs the ''rpoA'' RNA polymerase subunit from ''A. tumefaciens'' to work in ''Escherichia coli'' but this ''virG'' BioBrick is mutated, so works with the ''rpoA'' subunit from ''E. coli''. false false _517_ 0 6670 9 It's complicated false synthesized by Mr. Gene, so optimated codon usage, removal of illegal restriction sites false Jonas Aretz annotation2078361 1 virG range2078361 1 12 737 annotation2078362 1 Stop codon range2078362 1 738 740 annotation2078360 1 RBS range2078360 1 4 8 BBa_K389001 1 virA VirA receptor 2010-08-09T11:00:00Z 2015-05-08T01:12:18Z ''Agrobacterium tumefaciens'' C58, TI-plasmid This is a gene for the ''virA'' receptor from ''Agrobacterium tumefaciens'' C58. An illegal ''PstI'' restriction site was removed by site-directed mutagenesis. false false _517_ 0 6670 9 It's complicated false Illegal ''PstI'' restriction site removed false Jonas Aretz annotation2077044 1 virA range2077044 1 1 2505 annotation2078363 1 Stop codon range2078363 1 2503 2505 annotation2077043 1 Illegal PstI site removed range2077043 1 187 187 BBa_E1010 1 mRFP1 **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral) 2004-07-27T11:00:00Z 2015-08-31T04:07:26Z Campbell et al., PNAS v99 p7877 <a href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12060735">URL</a> Released HQ 2013 monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm false false _11_1_ 0 52 7 In stock false TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A. true Drew Endy annotation2214014 1 Help:Barcodes range2214014 1 682 706 annotation1014044 1 mrfp1 range1014044 1 1 675 BBa_K389010 1 virA VirA receptor (expressed) 2010-08-09T11:00:00Z 2015-05-08T01:12:19Z ''A. tumefaciens'' C58, partsregistry This BioBrick contains a ''virA'' receptor from ''Agrobacterium tumefaciens'' C58 under the control of a constitutive promotor. We used it to mutate ''virA'' by error-prone PCR. For screening the mutants BBa_K389011 is also needed. false false _517_ 0 6670 9 It's complicated false The ''virA'' gene is under the control a medium strong constitutive promotor. false Jonas Aretz component2219527 1 BBa_J23110 component2219533 1 BBa_K389001 component2219529 1 BBa_B0034 annotation2219529 1 BBa_B0034 range2219529 1 44 55 annotation2219527 1 BBa_J23110 range2219527 1 1 35 annotation2219533 1 BBa_K389001 range2219533 1 62 2566 BBa_K389423 1 BBa_K389423 VirA/G signaling system + sensitivity tuner + luciferase 2010-10-10T11:00:00Z 2015-05-08T01:12:19Z * luciferase from pGL4.10luc2 (Promega) * sensitivity tuner part, terminators and constitutive promoter from the partsregistry * ''virG'' was synthesized by Mr. Gene * ''virA'' and ''vir'' promoter from ''Agrobacterium tumefaciens'' C58, TI-plasmid This part combines a complete VirA/G signaling system with a sensitivity tuner before a luciferase gene under the control of a ''vir'' promoter. The sensitivity tuner enhances a signal from a promoter so it enhances the readout luciferase. false false _517_ 0 6670 9 It's complicated false sensitivity tuner to enhance the luciferase signal false Jonas Aretz component2313969 1 BBa_K389403 component2313944 1 BBa_K389017 annotation2313969 1 BBa_K389403 range2313969 1 3708 6621 annotation2313944 1 BBa_K389017 range2313944 1 1 3699 BBa_I746352 1 BBa_I746352 delta activator from phiR73 phage 2007-09-11T11:00:00Z 2015-08-31T04:08:04Z plasmid DNA supplied by Prof. Richard Calendar, University of California. The delta activator taken from phiR73 phage acts on a class of inducible promoters (parts I746360 to I746365), inducing their activity to varying degrees. The part sequence does already contain a ribosome binding site (B0034)! false false _116_ 0 2122 9 In stock true The part does contain a RBS (B0034) already. true Stefan Milde annotation1943873 1 phiR73 delta range1943873 1 22 22 annotation1943874 1 silent mutation to remove PstI site range1943874 1 102 102 annotation1943872 1 phiR73 delta range1943872 1 22 264 annotation1943871 1 B0034 range1943871 1 1 12 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0017 1 BBa_B0017 double terminator (B0010-B0010) 2004-01-08T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator with two copies of <bb_part>BBa_B0010</bb_part> false false _1_ 0 24 7 In stock false true Austin Che component939337 1 BBa_B0010 component939331 1 BBa_B0010 annotation939331 1 BBa_B0010 range939331 1 1 80 annotation939337 1 BBa_B0010 range939337 1 89 168 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 BBa_K389003 1 Pvir virB promoter 2010-08-09T11:00:00Z 2015-05-08T01:12:19Z ''A. tumefaciens'' C58, TI-plasmid This is a ''vir'' promotor from ''Agrobacterium tumefaciens'' C58. It is located on the TI-plasmid before the ''virB'' genes. It is activated by phosphorylated ''virG'' response regulator. false false _517_ 0 6670 9 It's complicated false contains already a RBS false Jonas Aretz annotation2077848 1 vir-box range2077848 1 33 44 annotation2077849 1 rbs range2077849 1 153 157 annotation2077851 1 -10 range2077851 1 113 117 annotation2077850 1 -35 range2077850 1 89 93 BBa_I746360 1 BBa_I746360 PF promoter from P2 phage 2007-09-10T11:00:00Z 2015-08-31T04:08:04Z The original source is the P2 phage genome. For our work the DNA came from plasmids, kindly supplied to us by Prof. Calendar, University of California (Berkeley): Bacteriophage PSP3 and fR73 Activator Proteins: Analysis of Promoter Specificities; Julien and Calendar, 1996; JOURNAL OF BACTERIOLOGY, Oct. 1996, p. 5668???5675 Released HQ 2013 This is the PF promoter taken from the P2 phage genome. It is an inducible promoter that is activated by a class of activators, including P2 ogr (I746350), PSP3 pag (I746351) and phiR73 delta (I746352). These different activators should cause different levels of activity of the PF promoter. false false _116_ 0 2122 9 In stock false no special considerations seemed necessary true Stefan Milde annotation1943783 1 PF range1943783 1 1 91 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_K389004 1 luc Luciferase from pGL4.10[luc2] 2010-08-09T11:00:00Z 2015-05-08T01:12:19Z pGL4.10luc2 A luciferase from Promega's pGL4.10luc2 vector. false false _517_ 0 6670 9 It's complicated true sequence of this vector is already published by Promega false Jonas Aretz annotation2078364 1 Stop codon range2078364 1 1654 1656 annotation2077057 1 Luciferase range2077057 1 1 1653 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K389403 1 BBa_K389403 Sensitivity tuner + luciferase 2010-10-10T11:00:00Z 2015-05-08T01:12:19Z * luciferase from pGL4.10luc2 (Promega) * the rest from the partsregistry This is a modified <partinfo>BBa_I746390</partinfo> sensitivity tuner. Instead of a GFP reporter, this one has a firefly luciferase (from Promega's pGL4.10luc2 vector) as one reporter gene. The arabinose promoter has been removed from the original part so now it is possible to assemble this part behind any promoter to get an enhanced luciferase signal. false false _517_ 0 6670 9 It's complicated false arabinose promoter removed, GFP exchanged with luciferase compared to the original part <partinfo>BBa_I746370</partinfo> false Jonas Aretz component2273115 1 BBa_I746360 component2273117 1 BBa_B0034 component2273106 1 BBa_E1010 component2273101 1 BBa_I746352 component2273103 1 BBa_B0034 component2273120 1 BBa_K389004 component2273113 1 BBa_B0015 annotation2273103 1 BBa_B0034 range2273103 1 273 284 annotation2273101 1 BBa_I746352 range2273101 1 1 264 annotation2273117 1 BBa_B0034 range2273117 1 1241 1252 annotation2273113 1 BBa_B0015 range2273113 1 1005 1133 annotation2273120 1 BBa_K389004 range2273120 1 1259 2914 annotation2273106 1 BBa_E1010 range2273106 1 291 996 annotation2273115 1 BBa_I746360 range2273115 1 1142 1232 BBa_K389002_sequence 1 aagaggagaaaatgaaacacgtcctgctggttgatgatgatgtggctatgcgtcacctgattatcgagtatctgaccatccatgccttcaaagtcacagccgttgccgattccactcagtttacccgtgtactgtctagtgctaccgtcgatgttgtcgtggtcgatctgaatctggttcgtgaagatggcctggaaattgtccgtaacctggcagcaaaaagcgatatccctatcattgtgatcagcggggatcgtctggaggaaacagacaaagttgttgccctggagctgggtgcttctgattttattgccaaaccgttcagcatccgtgagtttctggcccgtattcgtgtagccctgcgcgttcgtcctaacgtcgttcgttcgaaagaccgtcgttccttttgttttaccgactggaccctgaatctgcgtcaacgtcgcctgatgagcgaagctggcggtgaagtgaaactgacagccggtgagttcaatctgctgctggcatttctggaaaaacctcgtgatgttctgagccgtgaacagctgctgattgcctctcgtgtccgtgatgaagaggtgtatgatcgttctatcgatgtcctgattctgcgcctgcgtcgcaaactggaagccgatccgtctagtcctcagctgattaaaaccgcccgtggtgctgggtatttcttcgatgccgatgtccaagtgtcacatggtggcacgatggctgcttaataa BBa_K389403_sequence 1 aaagaggagaaatactagatgatgcgctgccctttctgtcgtcattcagcgcatacccgcaccagccggtatgtgagtgacaatgtcaaagaaagttatctccagtgccagaatatttactgttcggcgacatttaaaacgcatgagtcaatttgtgccgtgattcgttctccggtcacggaggaaaaaccagcaccggcaagcacagcaccggctgttgtccgaaaagttaaaggctgttacagctcaccattcaaccattaatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgcccgccttttctttaccggtggttgtgctgtcgattagccaaccgggacaaatagcctgacatctccggcgcaactgaaaataccacttactagagaaagaggagaaatactagatggaagatgccaaaaacattaagaagggcccagcgccattctacccactcgaagacgggaccgccggcgagcagctgcacaaagccatgaagcgctacgccctggtgcccggcaccatcgcctttaccgacgcacatatcgaggtggacattacctacgccgagtacttcgagatgagcgttcggctggcagaagctatgaagcgctatgggctgaatacaaaccatcggatcgtggtgtgcagcgagaatagcttgcagttcttcatgcccgtgttgggtgccctgttcatcggtgtggctgtggccccagctaacgacatctacaacgagcgcgagctgctgaacagcatgggcatcagccagcccaccgtcgtattcgtgagcaagaaagggctgcaaaagatcctcaacgtgcaaaagaagctaccgatcatacaaaagatcatcatcatggatagcaagaccgactaccagggcttccaaagcatgtacaccttcgtgacttcccatttgccacccggcttcaacgagtacgacttcgtgcccgagagcttcgaccgggacaaaaccatcgccctgatcatgaacagtagtggcagtaccggattgcccaagggcgtagccctaccgcaccgcaccgcttgtgtccgattcagtcatgcccgcgaccccatcttcggcaaccagatcatccccgacaccgctatcctcagcgtggtgccatttcaccacggcttcggcatgttcaccacgctgggctacttgatctgcggctttcgggtcgtgctcatgtaccgcttcgaggaggagctattcttgcgcagcttgcaagactataagattcaatctgccctgctggtgcccacactatttagcttcttcgctaagagcactctcatcgacaagtacgacctaagcaacttgcacgagatcgccagcggcggggcgccgctcagcaaggaggtaggtgaggccgtggccaaacgcttccacctaccaggcatccgccagggctacggcctgacagaaacaaccagcgccattctgatcacccccgaaggggacgacaagcctggcgcagtaggcaaggtggtgcccttcttcgaggctaaggtggtggacttggacaccggtaagacactgggtgtgaaccagcgcggcgagctgtgcgtccgtggccccatgatcatgagcggctacgttaacaaccccgaggctacaaacgctctcatcgacaaggacggctggctgcacagcggcgacatcgcctactgggacgaggacgagcacttcttcatcgtggaccggctgaagagcctgatcaaatacaagggctaccaggtagccccagccgaactggagagcatcctgctgcaacaccccaacatcttcgacgccggggtcgccggcctgcccgacgacgatgccggcgagctgcccgccgcagtcgtcgtgctggaacacggtaaaaccatgaccgagaaggagatcgtggactatgtggccagccaggttacaaccgccaagaagctgcgcggtggtgttgtgttcgtggacgaggtgcctaaaggactgaccggcaagttggacgcccgcaagatccgcgagattctcattaaggccaagaagggcggcaagatcgccgtgtaataa BBa_K389010_sequence 1 tttacggctagctcagtcctaggtacaatgctagctactagagaaagaggagaaatactagatgaatggaaggtattcaccgtctcggcaagatttcaagacaggcgccaagccttggtctatcctggccttggtagttgctgcaatgatttttgccttgatggcgattacgtcttggcaggacaatgaaaccaatcgggcgatcctgacccaattgcgagctattaacatcgacagtgcttcgctccagcgggatgtactccgcgcggaagcgggtgtggtggcgaactaccggcccattatctccaggttgggagctttgcggaagaacctggaaaatttgaagcgactatttaaacaatctcatcttgtgatcggcaatgatttctctcaactgctcgacaagctaaaggtgtctgtggatacgaccgacgcggccgttgcagccttcggagcgcaaaacgtgctcctgcaagattcgcttgccagcttcactcgcgcgcttagtattcttcccaaaatgtcgtcgacggatcagacggtcgaaaattcgaacgaattgggcagcctgatgctgcggtttgtgcgtcagccaagcccagcactctcgttggagatcagtcacgaactcgacatgctccaaaaagctagcggtggggctgaagttcctatccgtatacttgcacgcgagggtcgcgtcatcttgtcgattttgccccgtgtgaacgatgccgtaaacatgattcagacctccgacaccgctgaaattgccgaaagattggagcgcaagtgtttggaggcctatagcttgcaaagcgtgagggagcagcgggcacggatcttcttgggttccgtttcggtgggcctttgcatctacatcatctcactggtctataggctgcgtcggaaaacggcttggttaacgcggcgtttggattacgaagaggtaatcaaagagattggggtttgtttcgaggggggaggggccacagcgtcgtccctcaattcgtccgcgcaagctgcgcttggaattattcaacgcttctttaatgcggaatcgtgtgcactagcattggtggaccatggtgacaggtgggctgtcgaaagtttcgctgcgaagctgcctgagcccgtctgggaggacctcgcgctacgcgagatggtttctcttgccagagcggatgagcgtgcgtcagtattccgcatcatgtcgacgcgaaaggtcagctgccttcctccggagactccgggcgtttctatgctgctggcacacaaatctacggatcaactgatagcgatttgttccctcggttaccagggctatcgtctgaaatcttgtccaggcgaagttcagcttcttgaactcgccaccgcctgcctctgccactatatcgatgtccggcgtaagcagaccgaatgcgatattctggagaggcgattagagcatgcggaacgtcttcaggcagttggtacacttgctggtggaatagcgcatgagttcaacaacattttgggagcaatcctcgggtacgctgaaatggcgcaaaacatgctgcgtcggtcatctgtcacccgaagacacattgaccaaattatttcgtcgggtgacagagccaggctcattatcgatcagatcttgactctaagtcgaaaactagaacgcgtgacaaagccgttcagtgtctccgaactcgtaatggaaattgctcccttattgcgtgttgcattgcagcgcaacatcgagctgaagttcaagtttgacgacaagaagagcgtagtcgaaggaagcccgctagaggttcagcagatgctgatgaatctttgcaagaacgcttcccaggcgtttaccgccgatggtcaaatcgacatcatcgttagccgaatttttgtatctcgacagaaagtactggcgcatggtgttatgccagctggcgactatgttcttctttctgtcagcgacgacggtgaaggcattgccgaaactgtgctcccccacatttttgagcctttctttacaacacgctcttgcagcggcggcacgggtctaggtcttgctgccgtgcacggtcatgtcagcgcgcttgcaggatatattgacgttacttcagccgtagggcgagggacgcgcttcgacatttatctacctccttcctcgaagaagcccgtcagcccggacgcgttttttgggccctgtaaaacaccgcgtggcaacggagaaattgtggcattgattgagccagatcctgtcttgcgggaggtgtacgaagacaagatcgccgctctgggctatgagccggtgggctttaagacatgtgcagacctttgcaattggatatcaaaaggcaagcaagccgatctggttctagttgaccaatcgtctcttcccgagaatcagagtgctactgctttgcacgcagccttcaagacggcgtccatcatcattggaggaagtgatcttaaaatgtcactttccagcgatgacatgacgtccgcgctttttctgcccaaacctatatcctccaggacgatggcctacgcgattcgtaccaagatcaaagcctaataa BBa_B0017_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0034_sequence 1 aaagaggagaaa BBa_K389004_sequence 1 atggaagatgccaaaaacattaagaagggcccagcgccattctacccactcgaagacgggaccgccggcgagcagctgcacaaagccatgaagcgctacgccctggtgcccggcaccatcgcctttaccgacgcacatatcgaggtggacattacctacgccgagtacttcgagatgagcgttcggctggcagaagctatgaagcgctatgggctgaatacaaaccatcggatcgtggtgtgcagcgagaatagcttgcagttcttcatgcccgtgttgggtgccctgttcatcggtgtggctgtggccccagctaacgacatctacaacgagcgcgagctgctgaacagcatgggcatcagccagcccaccgtcgtattcgtgagcaagaaagggctgcaaaagatcctcaacgtgcaaaagaagctaccgatcatacaaaagatcatcatcatggatagcaagaccgactaccagggcttccaaagcatgtacaccttcgtgacttcccatttgccacccggcttcaacgagtacgacttcgtgcccgagagcttcgaccgggacaaaaccatcgccctgatcatgaacagtagtggcagtaccggattgcccaagggcgtagccctaccgcaccgcaccgcttgtgtccgattcagtcatgcccgcgaccccatcttcggcaaccagatcatccccgacaccgctatcctcagcgtggtgccatttcaccacggcttcggcatgttcaccacgctgggctacttgatctgcggctttcgggtcgtgctcatgtaccgcttcgaggaggagctattcttgcgcagcttgcaagactataagattcaatctgccctgctggtgcccacactatttagcttcttcgctaagagcactctcatcgacaagtacgacctaagcaacttgcacgagatcgccagcggcggggcgccgctcagcaaggaggtaggtgaggccgtggccaaacgcttccacctaccaggcatccgccagggctacggcctgacagaaacaaccagcgccattctgatcacccccgaaggggacgacaagcctggcgcagtaggcaaggtggtgcccttcttcgaggctaaggtggtggacttggacaccggtaagacactgggtgtgaaccagcgcggcgagctgtgcgtccgtggccccatgatcatgagcggctacgttaacaaccccgaggctacaaacgctctcatcgacaaggacggctggctgcacagcggcgacatcgcctactgggacgaggacgagcacttcttcatcgtggaccggctgaagagcctgatcaaatacaagggctaccaggtagccccagccgaactggagagcatcctgctgcaacaccccaacatcttcgacgccggggtcgccggcctgcccgacgacgatgccggcgagctgcccgccgcagtcgtcgtgctggaacacggtaaaaccatgaccgagaaggagatcgtggactatgtggccagccaggttacaaccgccaagaagctgcgcggtggtgttgtgttcgtggacgaggtgcctaaaggactgaccggcaagttggacgcccgcaagatccgcgagattctcattaaggccaagaagggcggcaagatcgccgtgtaataa BBa_E1010_sequence 1 atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc BBa_K389017_sequence 1 tttacggctagctcagtcctaggtacaatgctagctactagagaaagaggagaaatactagatgaatggaaggtattcaccgtctcggcaagatttcaagacaggcgccaagccttggtctatcctggccttggtagttgctgcaatgatttttgccttgatggcgattacgtcttggcaggacaatgaaaccaatcgggcgatcctgacccaattgcgagctattaacatcgacagtgcttcgctccagcgggatgtactccgcgcggaagcgggtgtggtggcgaactaccggcccattatctccaggttgggagctttgcggaagaacctggaaaatttgaagcgactatttaaacaatctcatcttgtgatcggcaatgatttctctcaactgctcgacaagctaaaggtgtctgtggatacgaccgacgcggccgttgcagccttcggagcgcaaaacgtgctcctgcaagattcgcttgccagcttcactcgcgcgcttagtattcttcccaaaatgtcgtcgacggatcagacggtcgaaaattcgaacgaattgggcagcctgatgctgcggtttgtgcgtcagccaagcccagcactctcgttggagatcagtcacgaactcgacatgctccaaaaagctagcggtggggctgaagttcctatccgtatacttgcacgcgagggtcgcgtcatcttgtcgattttgccccgtgtgaacgatgccgtaaacatgattcagacctccgacaccgctgaaattgccgaaagattggagcgcaagtgtttggaggcctatagcttgcaaagcgtgagggagcagcgggcacggatcttcttgggttccgtttcggtgggcctttgcatctacatcatctcactggtctataggctgcgtcggaaaacggcttggttaacgcggcgtttggattacgaagaggtaatcaaagagattggggtttgtttcgaggggggaggggccacagcgtcgtccctcaattcgtccgcgcaagctgcgcttggaattattcaacgcttctttaatgcggaatcgtgtgcactagcattggtggaccatggtgacaggtgggctgtcgaaagtttcgctgcgaagctgcctgagcccgtctgggaggacctcgcgctacgcgagatggtttctcttgccagagcggatgagcgtgcgtcagtattccgcatcatgtcgacgcgaaaggtcagctgccttcctccggagactccgggcgtttctatgctgctggcacacaaatctacggatcaactgatagcgatttgttccctcggttaccagggctatcgtctgaaatcttgtccaggcgaagttcagcttcttgaactcgccaccgcctgcctctgccactatatcgatgtccggcgtaagcagaccgaatgcgatattctggagaggcgattagagcatgcggaacgtcttcaggcagttggtacacttgctggtggaatagcgcatgagttcaacaacattttgggagcaatcctcgggtacgctgaaatggcgcaaaacatgctgcgtcggtcatctgtcacccgaagacacattgaccaaattatttcgtcgggtgacagagccaggctcattatcgatcagatcttgactctaagtcgaaaactagaacgcgtgacaaagccgttcagtgtctccgaactcgtaatggaaattgctcccttattgcgtgttgcattgcagcgcaacatcgagctgaagttcaagtttgacgacaagaagagcgtagtcgaaggaagcccgctagaggttcagcagatgctgatgaatctttgcaagaacgcttcccaggcgtttaccgccgatggtcaaatcgacatcatcgttagccgaatttttgtatctcgacagaaagtactggcgcatggtgttatgccagctggcgactatgttcttctttctgtcagcgacgacggtgaaggcattgccgaaactgtgctcccccacatttttgagcctttctttacaacacgctcttgcagcggcggcacgggtctaggtcttgctgccgtgcacggtcatgtcagcgcgcttgcaggatatattgacgttacttcagccgtagggcgagggacgcgcttcgacatttatctacctccttcctcgaagaagcccgtcagcccggacgcgttttttgggccctgtaaaacaccgcgtggcaacggagaaattgtggcattgattgagccagatcctgtcttgcgggaggtgtacgaagacaagatcgccgctctgggctatgagccggtgggctttaagacatgtgcagacctttgcaattggatatcaaaaggcaagcaagccgatctggttctagttgaccaatcgtctcttcccgagaatcagagtgctactgctttgcacgcagccttcaagacggcgtccatcatcattggaggaagtgatcttaaaatgtcactttccagcgatgacatgacgtccgcgctttttctgcccaaacctatatcctccaggacgatggcctacgcgattcgtaccaagatcaaagcctaataatactagagtttacggctagctcagtcctaggtacaatgctagctactagagaagaggagaaaatgaaacacgtcctgctggttgatgatgatgtggctatgcgtcacctgattatcgagtatctgaccatccatgccttcaaagtcacagccgttgccgattccactcagtttacccgtgtactgtctagtgctaccgtcgatgttgtcgtggtcgatctgaatctggttcgtgaagatggcctggaaattgtccgtaacctggcagcaaaaagcgatatccctatcattgtgatcagcggggatcgtctggaggaaacagacaaagttgttgccctggagctgggtgcttctgattttattgccaaaccgttcagcatccgtgagtttctggcccgtattcgtgtagccctgcgcgttcgtcctaacgtcgttcgttcgaaagaccgtcgttccttttgttttaccgactggaccctgaatctgcgtcaacgtcgcctgatgagcgaagctggcggtgaagtgaaactgacagccggtgagttcaatctgctgctggcatttctggaaaaacctcgtgatgttctgagccgtgaacagctgctgattgcctctcgtgtccgtgatgaagaggtgtatgatcgttctatcgatgtcctgattctgcgcctgcgtcgcaaactggaagccgatccgtctagtcctcagctgattaaaaccgcccgtggtgctgggtatttcttcgatgccgatgtccaagtgtcacatggtggcacgatggctgcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagaggcacccttaagtgcatggaaagccgttttcgcttcaaatgaaatcgaaaagaagaaaacaaaaatcctagagtaaccgaccctcccgataatcgtgaacatcagatgacagcatttcttccgaccgaagtggctgtgttggttatgagcttgaggaga BBa_I746360_sequence 1 ttgcccgccttttctttaccggtggttgtgctgtcgattagccaaccgggacaaatagcctgacatctccggcgcaactgaaaataccact BBa_I746352_sequence 1 aaagaggagaaatactagatgatgcgctgccctttctgtcgtcattcagcgcatacccgcaccagccggtatgtgagtgacaatgtcaaagaaagttatctccagtgccagaatatttactgttcggcgacatttaaaacgcatgagtcaatttgtgccgtgattcgttctccggtcacggaggaaaaaccagcaccggcaagcacagcaccggctgttgtccgaaaagttaaaggctgttacagctcaccattcaaccattaa BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K389003_sequence 1 gcacccttaagtgcatggaaagccgttttcgcttcaaatgaaatcgaaaagaagaaaacaaaaatcctagagtaaccgaccctcccgataatcgtgaacatcagatgacagcatttcttccgaccgaagtggctgtgttggttatgagcttgaggaga BBa_K389423_sequence 1 tttacggctagctcagtcctaggtacaatgctagctactagagaaagaggagaaatactagatgaatggaaggtattcaccgtctcggcaagatttcaagacaggcgccaagccttggtctatcctggccttggtagttgctgcaatgatttttgccttgatggcgattacgtcttggcaggacaatgaaaccaatcgggcgatcctgacccaattgcgagctattaacatcgacagtgcttcgctccagcgggatgtactccgcgcggaagcgggtgtggtggcgaactaccggcccattatctccaggttgggagctttgcggaagaacctggaaaatttgaagcgactatttaaacaatctcatcttgtgatcggcaatgatttctctcaactgctcgacaagctaaaggtgtctgtggatacgaccgacgcggccgttgcagccttcggagcgcaaaacgtgctcctgcaagattcgcttgccagcttcactcgcgcgcttagtattcttcccaaaatgtcgtcgacggatcagacggtcgaaaattcgaacgaattgggcagcctgatgctgcggtttgtgcgtcagccaagcccagcactctcgttggagatcagtcacgaactcgacatgctccaaaaagctagcggtggggctgaagttcctatccgtatacttgcacgcgagggtcgcgtcatcttgtcgattttgccccgtgtgaacgatgccgtaaacatgattcagacctccgacaccgctgaaattgccgaaagattggagcgcaagtgtttggaggcctatagcttgcaaagcgtgagggagcagcgggcacggatcttcttgggttccgtttcggtgggcctttgcatctacatcatctcactggtctataggctgcgtcggaaaacggcttggttaacgcggcgtttggattacgaagaggtaatcaaagagattggggtttgtttcgaggggggaggggccacagcgtcgtccctcaattcgtccgcgcaagctgcgcttggaattattcaacgcttctttaatgcggaatcgtgtgcactagcattggtggaccatggtgacaggtgggctgtcgaaagtttcgctgcgaagctgcctgagcccgtctgggaggacctcgcgctacgcgagatggtttctcttgccagagcggatgagcgtgcgtcagtattccgcatcatgtcgacgcgaaaggtcagctgccttcctccggagactccgggcgtttctatgctgctggcacacaaatctacggatcaactgatagcgatttgttccctcggttaccagggctatcgtctgaaatcttgtccaggcgaagttcagcttcttgaactcgccaccgcctgcctctgccactatatcgatgtccggcgtaagcagaccgaatgcgatattctggagaggcgattagagcatgcggaacgtcttcaggcagttggtacacttgctggtggaatagcgcatgagttcaacaacattttgggagcaatcctcgggtacgctgaaatggcgcaaaacatgctgcgtcggtcatctgtcacccgaagacacattgaccaaattatttcgtcgggtgacagagccaggctcattatcgatcagatcttgactctaagtcgaaaactagaacgcgtgacaaagccgttcagtgtctccgaactcgtaatggaaattgctcccttattgcgtgttgcattgcagcgcaacatcgagctgaagttcaagtttgacgacaagaagagcgtagtcgaaggaagcccgctagaggttcagcagatgctgatgaatctttgcaagaacgcttcccaggcgtttaccgccgatggtcaaatcgacatcatcgttagccgaatttttgtatctcgacagaaagtactggcgcatggtgttatgccagctggcgactatgttcttctttctgtcagcgacgacggtgaaggcattgccgaaactgtgctcccccacatttttgagcctttctttacaacacgctcttgcagcggcggcacgggtctaggtcttgctgccgtgcacggtcatgtcagcgcgcttgcaggatatattgacgttacttcagccgtagggcgagggacgcgcttcgacatttatctacctccttcctcgaagaagcccgtcagcccggacgcgttttttgggccctgtaaaacaccgcgtggcaacggagaaattgtggcattgattgagccagatcctgtcttgcgggaggtgtacgaagacaagatcgccgctctgggctatgagccggtgggctttaagacatgtgcagacctttgcaattggatatcaaaaggcaagcaagccgatctggttctagttgaccaatcgtctcttcccgagaatcagagtgctactgctttgcacgcagccttcaagacggcgtccatcatcattggaggaagtgatcttaaaatgtcactttccagcgatgacatgacgtccgcgctttttctgcccaaacctatatcctccaggacgatggcctacgcgattcgtaccaagatcaaagcctaataatactagagtttacggctagctcagtcctaggtacaatgctagctactagagaagaggagaaaatgaaacacgtcctgctggttgatgatgatgtggctatgcgtcacctgattatcgagtatctgaccatccatgccttcaaagtcacagccgttgccgattccactcagtttacccgtgtactgtctagtgctaccgtcgatgttgtcgtggtcgatctgaatctggttcgtgaagatggcctggaaattgtccgtaacctggcagcaaaaagcgatatccctatcattgtgatcagcggggatcgtctggaggaaacagacaaagttgttgccctggagctgggtgcttctgattttattgccaaaccgttcagcatccgtgagtttctggcccgtattcgtgtagccctgcgcgttcgtcctaacgtcgttcgttcgaaagaccgtcgttccttttgttttaccgactggaccctgaatctgcgtcaacgtcgcctgatgagcgaagctggcggtgaagtgaaactgacagccggtgagttcaatctgctgctggcatttctggaaaaacctcgtgatgttctgagccgtgaacagctgctgattgcctctcgtgtccgtgatgaagaggtgtatgatcgttctatcgatgtcctgattctgcgcctgcgtcgcaaactggaagccgatccgtctagtcctcagctgattaaaaccgcccgtggtgctgggtatttcttcgatgccgatgtccaagtgtcacatggtggcacgatggctgcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagaggcacccttaagtgcatggaaagccgttttcgcttcaaatgaaatcgaaaagaagaaaacaaaaatcctagagtaaccgaccctcccgataatcgtgaacatcagatgacagcatttcttccgaccgaagtggctgtgttggttatgagcttgaggagatactagagaaagaggagaaatactagatgatgcgctgccctttctgtcgtcattcagcgcatacccgcaccagccggtatgtgagtgacaatgtcaaagaaagttatctccagtgccagaatatttactgttcggcgacatttaaaacgcatgagtcaatttgtgccgtgattcgttctccggtcacggaggaaaaaccagcaccggcaagcacagcaccggctgttgtccgaaaagttaaaggctgttacagctcaccattcaaccattaatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgcccgccttttctttaccggtggttgtgctgtcgattagccaaccgggacaaatagcctgacatctccggcgcaactgaaaataccacttactagagaaagaggagaaatactagatggaagatgccaaaaacattaagaagggcccagcgccattctacccactcgaagacgggaccgccggcgagcagctgcacaaagccatgaagcgctacgccctggtgcccggcaccatcgcctttaccgacgcacatatcgaggtggacattacctacgccgagtacttcgagatgagcgttcggctggcagaagctatgaagcgctatgggctgaatacaaaccatcggatcgtggtgtgcagcgagaatagcttgcagttcttcatgcccgtgttgggtgccctgttcatcggtgtggctgtggccccagctaacgacatctacaacgagcgcgagctgctgaacagcatgggcatcagccagcccaccgtcgtattcgtgagcaagaaagggctgcaaaagatcctcaacgtgcaaaagaagctaccgatcatacaaaagatcatcatcatggatagcaagaccgactaccagggcttccaaagcatgtacaccttcgtgacttcccatttgccacccggcttcaacgagtacgacttcgtgcccgagagcttcgaccgggacaaaaccatcgccctgatcatgaacagtagtggcagtaccggattgcccaagggcgtagccctaccgcaccgcaccgcttgtgtccgattcagtcatgcccgcgaccccatcttcggcaaccagatcatccccgacaccgctatcctcagcgtggtgccatttcaccacggcttcggcatgttcaccacgctgggctacttgatctgcggctttcgggtcgtgctcatgtaccgcttcgaggaggagctattcttgcgcagcttgcaagactataagattcaatctgccctgctggtgcccacactatttagcttcttcgctaagagcactctcatcgacaagtacgacctaagcaacttgcacgagatcgccagcggcggggcgccgctcagcaaggaggtaggtgaggccgtggccaaacgcttccacctaccaggcatccgccagggctacggcctgacagaaacaaccagcgccattctgatcacccccgaaggggacgacaagcctggcgcagtaggcaaggtggtgcccttcttcgaggctaaggtggtggacttggacaccggtaagacactgggtgtgaaccagcgcggcgagctgtgcgtccgtggccccatgatcatgagcggctacgttaacaaccccgaggctacaaacgctctcatcgacaaggacggctggctgcacagcggcgacatcgcctactgggacgaggacgagcacttcttcatcgtggaccggctgaagagcctgatcaaatacaagggctaccaggtagccccagccgaactggagagcatcctgctgcaacaccccaacatcttcgacgccggggtcgccggcctgcccgacgacgatgccggcgagctgcccgccgcagtcgtcgtgctggaacacggtaaaaccatgaccgagaaggagatcgtggactatgtggccagccaggttacaaccgccaagaagctgcgcggtggtgttgtgttcgtggacgaggtgcctaaaggactgaccggcaagttggacgcccgcaagatccgcgagattctcattaaggccaagaagggcggcaagatcgccgtgtaataa BBa_K389001_sequence 1 atgaatggaaggtattcaccgtctcggcaagatttcaagacaggcgccaagccttggtctatcctggccttggtagttgctgcaatgatttttgccttgatggcgattacgtcttggcaggacaatgaaaccaatcgggcgatcctgacccaattgcgagctattaacatcgacagtgcttcgctccagcgggatgtactccgcgcggaagcgggtgtggtggcgaactaccggcccattatctccaggttgggagctttgcggaagaacctggaaaatttgaagcgactatttaaacaatctcatcttgtgatcggcaatgatttctctcaactgctcgacaagctaaaggtgtctgtggatacgaccgacgcggccgttgcagccttcggagcgcaaaacgtgctcctgcaagattcgcttgccagcttcactcgcgcgcttagtattcttcccaaaatgtcgtcgacggatcagacggtcgaaaattcgaacgaattgggcagcctgatgctgcggtttgtgcgtcagccaagcccagcactctcgttggagatcagtcacgaactcgacatgctccaaaaagctagcggtggggctgaagttcctatccgtatacttgcacgcgagggtcgcgtcatcttgtcgattttgccccgtgtgaacgatgccgtaaacatgattcagacctccgacaccgctgaaattgccgaaagattggagcgcaagtgtttggaggcctatagcttgcaaagcgtgagggagcagcgggcacggatcttcttgggttccgtttcggtgggcctttgcatctacatcatctcactggtctataggctgcgtcggaaaacggcttggttaacgcggcgtttggattacgaagaggtaatcaaagagattggggtttgtttcgaggggggaggggccacagcgtcgtccctcaattcgtccgcgcaagctgcgcttggaattattcaacgcttctttaatgcggaatcgtgtgcactagcattggtggaccatggtgacaggtgggctgtcgaaagtttcgctgcgaagctgcctgagcccgtctgggaggacctcgcgctacgcgagatggtttctcttgccagagcggatgagcgtgcgtcagtattccgcatcatgtcgacgcgaaaggtcagctgccttcctccggagactccgggcgtttctatgctgctggcacacaaatctacggatcaactgatagcgatttgttccctcggttaccagggctatcgtctgaaatcttgtccaggcgaagttcagcttcttgaactcgccaccgcctgcctctgccactatatcgatgtccggcgtaagcagaccgaatgcgatattctggagaggcgattagagcatgcggaacgtcttcaggcagttggtacacttgctggtggaatagcgcatgagttcaacaacattttgggagcaatcctcgggtacgctgaaatggcgcaaaacatgctgcgtcggtcatctgtcacccgaagacacattgaccaaattatttcgtcgggtgacagagccaggctcattatcgatcagatcttgactctaagtcgaaaactagaacgcgtgacaaagccgttcagtgtctccgaactcgtaatggaaattgctcccttattgcgtgttgcattgcagcgcaacatcgagctgaagttcaagtttgacgacaagaagagcgtagtcgaaggaagcccgctagaggttcagcagatgctgatgaatctttgcaagaacgcttcccaggcgtttaccgccgatggtcaaatcgacatcatcgttagccgaatttttgtatctcgacagaaagtactggcgcatggtgttatgccagctggcgactatgttcttctttctgtcagcgacgacggtgaaggcattgccgaaactgtgctcccccacatttttgagcctttctttacaacacgctcttgcagcggcggcacgggtctaggtcttgctgccgtgcacggtcatgtcagcgcgcttgcaggatatattgacgttacttcagccgtagggcgagggacgcgcttcgacatttatctacctccttcctcgaagaagcccgtcagcccggacgcgttttttgggccctgtaaaacaccgcgtggcaacggagaaattgtggcattgattgagccagatcctgtcttgcgggaggtgtacgaagacaagatcgccgctctgggctatgagccggtgggctttaagacatgtgcagacctttgcaattggatatcaaaaggcaagcaagccgatctggttctagttgaccaatcgtctcttcccgagaatcagagtgctactgctttgcacgcagccttcaagacggcgtccatcatcattggaggaagtgatcttaaaatgtcactttccagcgatgacatgacgtccgcgctttttctgcccaaacctatatcctccaggacgatggcctacgcgattcgtaccaagatcaaagcctaataa BBa_J23110_sequence 1 tttacggctagctcagtcctaggtacaatgctagc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z