##gff-version 3
##sequence-region BBa_K389423 1 6621
BBa_K389423	.	engineered_region	3708	6621	.	+	0	ID=BBa_K389403;Name=BBa_K389403
BBa_K389423	.	terminator	4712	4840	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K389403
BBa_K389423	.	terminator	4712	4791	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K389423	.	engineered_region	4712	4791	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K389423	.	stem_loop	4723	4766	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K389423	.	terminator	4800	4840	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K389423	.	engineered_region	4800	4840	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K389423	.	polyA_site	4827	4840	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K389423	.	stem_loop	4807	4826	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K389423	.	stop_codon	4833	4833	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K389423	.	CDS	3998	4703	.	+	0	ID=BBa_E1010;Name=BBa_E1010;Parent=BBa_K389403
BBa_K389423	.	CDS	3998	4672	.	+	0	ID=annotation1014044;Name=mrfp1;Parent=BBa_E1010
BBa_K389423	.	engineered_tag	4679	4703	.	+	0	ID=annotation2214014;Name=Help:Barcodes;Parent=BBa_E1010
BBa_K389423	.	engineered_region	3708	3971	.	+	0	ID=BBa_I746352;Name=BBa_I746352;Parent=BBa_K389403
BBa_K389423	.	start_codon	3729	3729	.	+	0	ID=annotation1943873;Name=phiR73 delta;Parent=BBa_I746352
BBa_K389423	.	engineered_region	3708	3719	.	+	0	ID=annotation1943871;Name=B0034;Parent=BBa_I746352
BBa_K389423	.	sequence_alteration	3809	3809	.	+	0	ID=annotation1943874;Name=silent mutation to remove PstI site;Parent=BBa_I746352
BBa_K389423	.	CDS	3729	3971	.	+	0	ID=annotation1943872;Name=phiR73 delta;Parent=BBa_I746352
BBa_K389423	.	engineered_region	4966	6621	.	+	0	ID=BBa_K389004;Name=BBa_K389004;Parent=BBa_K389403
BBa_K389423	.	CDS	4966	6618	.	+	0	ID=annotation2077057;Name=Luciferase;Parent=BBa_K389004
BBa_K389423	.	stop_codon	6619	6621	.	+	0	ID=annotation2078364;Name=Stop codon;Parent=BBa_K389004
BBa_K389423	.	ribosome_entry_site	4948	4959	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K389403
BBa_K389423	.	conserved_region	4952	4955	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K389423	.	ribosome_entry_site	3980	3991	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K389403
BBa_K389423	.	conserved_region	3984	3987	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K389423	.	promoter	4849	4939	.	+	0	ID=BBa_I746360;Name=BBa_I746360;Parent=BBa_K389403
BBa_K389423	.	promoter	4849	4939	.	+	0	ID=annotation1943783;Name=PF;Parent=BBa_I746360
BBa_K389423	.	promoter	1	3699	.	+	0	ID=BBa_K389017;Name=BBa_K389017
BBa_K389423	.	engineered_region	1	2566	.	+	0	ID=BBa_K389010;Name=BBa_K389010;Parent=BBa_K389017
BBa_K389423	.	ribosome_entry_site	44	55	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K389010
BBa_K389423	.	conserved_region	48	51	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K389423	.	CDS	62	2566	.	+	0	ID=BBa_K389001;Name=BBa_K389001;Parent=BBa_K389010
BBa_K389423	.	CDS	62	2566	.	+	0	ID=annotation2077044;Name=virA;Parent=BBa_K389001
BBa_K389423	.	stop_codon	2564	2566	.	+	0	ID=annotation2078363;Name=Stop codon;Parent=BBa_K389001
BBa_K389423	.	sequence_alteration	248	248	.	+	0	ID=annotation2077043;Name=Illegal PstI site removed;Parent=BBa_K389001
BBa_K389423	.	promoter	1	35	.	+	0	ID=BBa_J23110;Name=BBa_J23110;Parent=BBa_K389010
BBa_K389423	.	promoter	3542	3699	.	+	0	ID=BBa_K389003;Name=BBa_K389003;Parent=BBa_K389017
BBa_K389423	.	promoter	3630	3634	.	+	0	ID=annotation2077850;Name=-35;Parent=BBa_K389003
BBa_K389423	.	non_covalent_binding_site	3574	3585	.	+	0	ID=annotation2077848;Name=vir-box;Parent=BBa_K389003
BBa_K389423	.	promoter	3654	3658	.	+	0	ID=annotation2077851;Name=-10;Parent=BBa_K389003
BBa_K389423	.	ribosome_entry_site	3694	3698	.	+	0	ID=annotation2077849;Name=rbs;Parent=BBa_K389003
BBa_K389423	.	terminator	3366	3533	.	+	0	ID=BBa_B0017;Name=BBa_B0017;Parent=BBa_K389017
BBa_K389423	.	terminator	3366	3445	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0017
BBa_K389423	.	engineered_region	3366	3445	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K389423	.	stem_loop	3377	3420	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K389423	.	terminator	3454	3533	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0017
BBa_K389423	.	engineered_region	3454	3533	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K389423	.	stem_loop	3465	3508	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K389423	.	promoter	2575	2609	.	+	0	ID=BBa_J23110;Name=BBa_J23110;Parent=BBa_K389017
BBa_K389423	.	CDS	2618	3357	.	+	0	ID=BBa_K389002;Name=BBa_K389002;Parent=BBa_K389017
BBa_K389423	.	stop_codon	3355	3357	.	+	0	ID=annotation2078362;Name=Stop codon;Parent=BBa_K389002
BBa_K389423	.	ribosome_entry_site	2621	2625	.	+	0	ID=annotation2078360;Name=RBS;Parent=BBa_K389002
BBa_K389423	.	CDS	2629	3354	.	+	0	ID=annotation2078361;Name=virG;Parent=BBa_K389002
>BBa_K389423
tttacggctagctcagtcctaggtacaatgctagctactagagaaagaggagaaatactagatgaatggaaggtattca
ccgtctcggcaagatttcaagacaggcgccaagccttggtctatcctggccttggtagttgctgcaatgatttttgcct
tgatggcgattacgtcttggcaggacaatgaaaccaatcgggcgatcctgacccaattgcgagctattaacatcgacag
tgcttcgctccagcgggatgtactccgcgcggaagcgggtgtggtggcgaactaccggcccattatctccaggttggga
gctttgcggaagaacctggaaaatttgaagcgactatttaaacaatctcatcttgtgatcggcaatgatttctctcaac
tgctcgacaagctaaaggtgtctgtggatacgaccgacgcggccgttgcagccttcggagcgcaaaacgtgctcctgca
agattcgcttgccagcttcactcgcgcgcttagtattcttcccaaaatgtcgtcgacggatcagacggtcgaaaattcg
aacgaattgggcagcctgatgctgcggtttgtgcgtcagccaagcccagcactctcgttggagatcagtcacgaactcg
acatgctccaaaaagctagcggtggggctgaagttcctatccgtatacttgcacgcgagggtcgcgtcatcttgtcgat
tttgccccgtgtgaacgatgccgtaaacatgattcagacctccgacaccgctgaaattgccgaaagattggagcgcaag
tgtttggaggcctatagcttgcaaagcgtgagggagcagcgggcacggatcttcttgggttccgtttcggtgggccttt
gcatctacatcatctcactggtctataggctgcgtcggaaaacggcttggttaacgcggcgtttggattacgaagaggt
aatcaaagagattggggtttgtttcgaggggggaggggccacagcgtcgtccctcaattcgtccgcgcaagctgcgctt
ggaattattcaacgcttctttaatgcggaatcgtgtgcactagcattggtggaccatggtgacaggtgggctgtcgaaa
gtttcgctgcgaagctgcctgagcccgtctgggaggacctcgcgctacgcgagatggtttctcttgccagagcggatga
gcgtgcgtcagtattccgcatcatgtcgacgcgaaaggtcagctgccttcctccggagactccgggcgtttctatgctg
ctggcacacaaatctacggatcaactgatagcgatttgttccctcggttaccagggctatcgtctgaaatcttgtccag
gcgaagttcagcttcttgaactcgccaccgcctgcctctgccactatatcgatgtccggcgtaagcagaccgaatgcga
tattctggagaggcgattagagcatgcggaacgtcttcaggcagttggtacacttgctggtggaatagcgcatgagttc
aacaacattttgggagcaatcctcgggtacgctgaaatggcgcaaaacatgctgcgtcggtcatctgtcacccgaagac
acattgaccaaattatttcgtcgggtgacagagccaggctcattatcgatcagatcttgactctaagtcgaaaactaga
acgcgtgacaaagccgttcagtgtctccgaactcgtaatggaaattgctcccttattgcgtgttgcattgcagcgcaac
atcgagctgaagttcaagtttgacgacaagaagagcgtagtcgaaggaagcccgctagaggttcagcagatgctgatga
atctttgcaagaacgcttcccaggcgtttaccgccgatggtcaaatcgacatcatcgttagccgaatttttgtatctcg
acagaaagtactggcgcatggtgttatgccagctggcgactatgttcttctttctgtcagcgacgacggtgaaggcatt
gccgaaactgtgctcccccacatttttgagcctttctttacaacacgctcttgcagcggcggcacgggtctaggtcttg
ctgccgtgcacggtcatgtcagcgcgcttgcaggatatattgacgttacttcagccgtagggcgagggacgcgcttcga
catttatctacctccttcctcgaagaagcccgtcagcccggacgcgttttttgggccctgtaaaacaccgcgtggcaac
ggagaaattgtggcattgattgagccagatcctgtcttgcgggaggtgtacgaagacaagatcgccgctctgggctatg
agccggtgggctttaagacatgtgcagacctttgcaattggatatcaaaaggcaagcaagccgatctggttctagttga
ccaatcgtctcttcccgagaatcagagtgctactgctttgcacgcagccttcaagacggcgtccatcatcattggagga
agtgatcttaaaatgtcactttccagcgatgacatgacgtccgcgctttttctgcccaaacctatatcctccaggacga
tggcctacgcgattcgtaccaagatcaaagcctaataatactagagtttacggctagctcagtcctaggtacaatgcta
gctactagagaagaggagaaaatgaaacacgtcctgctggttgatgatgatgtggctatgcgtcacctgattatcgagt
atctgaccatccatgccttcaaagtcacagccgttgccgattccactcagtttacccgtgtactgtctagtgctaccgt
cgatgttgtcgtggtcgatctgaatctggttcgtgaagatggcctggaaattgtccgtaacctggcagcaaaaagcgat
atccctatcattgtgatcagcggggatcgtctggaggaaacagacaaagttgttgccctggagctgggtgcttctgatt
ttattgccaaaccgttcagcatccgtgagtttctggcccgtattcgtgtagccctgcgcgttcgtcctaacgtcgttcg
ttcgaaagaccgtcgttccttttgttttaccgactggaccctgaatctgcgtcaacgtcgcctgatgagcgaagctggc
ggtgaagtgaaactgacagccggtgagttcaatctgctgctggcatttctggaaaaacctcgtgatgttctgagccgtg
aacagctgctgattgcctctcgtgtccgtgatgaagaggtgtatgatcgttctatcgatgtcctgattctgcgcctgcg
tcgcaaactggaagccgatccgtctagtcctcagctgattaaaaccgcccgtggtgctgggtatttcttcgatgccgat
gtccaagtgtcacatggtggcacgatggctgcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcg
aaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagccaggcatcaaataaaacgaaag
gctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagaggcacccttaagtgc
atggaaagccgttttcgcttcaaatgaaatcgaaaagaagaaaacaaaaatcctagagtaaccgaccctcccgataatc
gtgaacatcagatgacagcatttcttccgaccgaagtggctgtgttggttatgagcttgaggagatactagagaaagag
gagaaatactagatgatgcgctgccctttctgtcgtcattcagcgcatacccgcaccagccggtatgtgagtgacaatg
tcaaagaaagttatctccagtgccagaatatttactgttcggcgacatttaaaacgcatgagtcaatttgtgccgtgat
tcgttctccggtcacggaggaaaaaccagcaccggcaagcacagcaccggctgttgtccgaaaagttaaaggctgttac
agctcaccattcaaccattaatactagagaaagaggagaaatactagatggcttcctccgaagacgttatcaaagagtt
catgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccg
tacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgc
agttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaagg
tttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggt
gagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggtt
gggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaaga
cggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaacc
gacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcact
ccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcag
tcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgg
gtgggcctttctgcgtttatatactagagttgcccgccttttctttaccggtggttgtgctgtcgattagccaaccggg
acaaatagcctgacatctccggcgcaactgaaaataccacttactagagaaagaggagaaatactagatggaagatgcc
aaaaacattaagaagggcccagcgccattctacccactcgaagacgggaccgccggcgagcagctgcacaaagccatga
agcgctacgccctggtgcccggcaccatcgcctttaccgacgcacatatcgaggtggacattacctacgccgagtactt
cgagatgagcgttcggctggcagaagctatgaagcgctatgggctgaatacaaaccatcggatcgtggtgtgcagcgag
aatagcttgcagttcttcatgcccgtgttgggtgccctgttcatcggtgtggctgtggccccagctaacgacatctaca
acgagcgcgagctgctgaacagcatgggcatcagccagcccaccgtcgtattcgtgagcaagaaagggctgcaaaagat
cctcaacgtgcaaaagaagctaccgatcatacaaaagatcatcatcatggatagcaagaccgactaccagggcttccaa
agcatgtacaccttcgtgacttcccatttgccacccggcttcaacgagtacgacttcgtgcccgagagcttcgaccggg
acaaaaccatcgccctgatcatgaacagtagtggcagtaccggattgcccaagggcgtagccctaccgcaccgcaccgc
ttgtgtccgattcagtcatgcccgcgaccccatcttcggcaaccagatcatccccgacaccgctatcctcagcgtggtg
ccatttcaccacggcttcggcatgttcaccacgctgggctacttgatctgcggctttcgggtcgtgctcatgtaccgct
tcgaggaggagctattcttgcgcagcttgcaagactataagattcaatctgccctgctggtgcccacactatttagctt
cttcgctaagagcactctcatcgacaagtacgacctaagcaacttgcacgagatcgccagcggcggggcgccgctcagc
aaggaggtaggtgaggccgtggccaaacgcttccacctaccaggcatccgccagggctacggcctgacagaaacaacca
gcgccattctgatcacccccgaaggggacgacaagcctggcgcagtaggcaaggtggtgcccttcttcgaggctaaggt
ggtggacttggacaccggtaagacactgggtgtgaaccagcgcggcgagctgtgcgtccgtggccccatgatcatgagc
ggctacgttaacaaccccgaggctacaaacgctctcatcgacaaggacggctggctgcacagcggcgacatcgcctact
gggacgaggacgagcacttcttcatcgtggaccggctgaagagcctgatcaaatacaagggctaccaggtagccccagc
cgaactggagagcatcctgctgcaacaccccaacatcttcgacgccggggtcgccggcctgcccgacgacgatgccggc
gagctgcccgccgcagtcgtcgtgctggaacacggtaaaaccatgaccgagaaggagatcgtggactatgtggccagcc
aggttacaaccgccaagaagctgcgcggtggtgttgtgttcgtggacgaggtgcctaaaggactgaccggcaagttgga
cgcccgcaagatccgcgagattctcattaaggccaagaagggcggcaagatcgccgtgtaataa