##gff-version 3
##sequence-region BBa_K390000 1 2157
BBa_K390000	.	sequence_feature	2009	2014	.	+	0	ID=annotation2121040;Name=XhoI Site
BBa_K390000	.	stem_loop	2107	2126	.	+	0	ID=annotation2121045;Name=terminator stem loop
BBa_K390000	.	sequence_feature	216	221	.	+	0	ID=annotation2121031;Name=NdeI site
BBa_K390000	.	sequence_feature	2094	2097	.	+	0	ID=annotation2121044;Name=end of terminator
BBa_K390000	.	sequence_feature	230	235	.	+	0	ID=annotation2121033;Name=NheI Site
BBa_K390000	.	sequence_alteration	219	219	.	+	0	ID=annotation2121032;Name=g > a
BBa_K390000	.	CDS	261	875	.	+	0	ID=annotation2121035;Name=rep(pMB1) (rev)
BBa_K390000	.	sequence_alteration	2014	2014	.	+	0	ID=annotation2121042;Name=a > g
BBa_K390000	.	primer_binding_site	157	176	.	+	0	ID=annotation2121030;Name=VR
BBa_K390000	.	CDS	1025	1895	.	+	0	ID=annotation2121037;Name=AmpR (rev)
BBa_K390000	.	engineered_region	1	21	.	+	0	ID=annotation2121026;Name=suffix
BBa_K390000	.	sequence_feature	276	276	.	+	0	ID=annotation2121036;Name=Ori
BBa_K390000	.	primer_binding_site	2017	2037	.	+	0	ID=annotation2121043;Name=VF2
BBa_K390000	.	engineered_region	2136	2157	.	+	0	ID=annotation2121046;Name=prefix
BBa_K390000	.	stem_loop	30	64	.	+	0	ID=annotation2121028;Name=terminator stem loop
BBa_K390000	.	sequence_alteration	1995	1995	.	+	0	ID=annotation2121039;Name=t > a
BBa_K390000	.	sequence_alteration	232	232	.	+	0	ID=annotation2121034;Name=c > t
BBa_K390000	.	sequence_alteration	2010	2010	.	+	0	ID=annotation2121041;Name=c > t
BBa_K390000	.	sequence_feature	22	93	.	+	0	ID=annotation2121027;Name=E. coli his operon terminator
BBa_K390000	.	sequence_feature	34	35	.	+	0	ID=annotation2121029;Name=deletion of two bases
BBa_K390000	.	sequence_feature	1992	1997	.	+	0	ID=annotation2121038;Name=BamHI Site
>BBa_K390000
tactagtagcggccgctgcagtccggcaaaaaaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaa
gattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagc
tcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatatgagcaaaaggctagcaa
aaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatc
gacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcg
ctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagc
tcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccg
accgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccac
tggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacact
agaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggca
aacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaaga
tcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatca
aaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggt
ctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgact
ccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgc
tcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccg
cctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgc
cattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcga
gttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccg
cagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgac
tggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggat
aataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatct
taccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgt
ttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcata
ctcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttaga
aaaataaacaaataggggatccgcgcacatttcctcgagaagtgccacctgacgtctaagaaaccattattatcatgac
attaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttc
tggaattcgcggccgcttctagag