BBa_K390006
1
BBa_K390006
sigA Type I promoter from Synechocystis sp. PCC 6803
2010-07-31T11:00:00Z
2015-05-08T01:12:19Z
Sequence for genomic DNA, part assembled by annealing of two oligionucleotides.
This is the promoter for the sigma factor ''sig''A in ''Synechocystis'' sp. PCC 6803. It is self-regulating (controlled by SigA), but has shown to potentially be down-regulated due to changes from heat-shock, darkness, and salt stress. A decrease in SigA protein was observed in a SigC knockout, but not direct proportion to the transcript of SigA (Imamura S and Asayama M. 2009. Sigma Factors for Cyanobacterial Transcription. Gene Regulation and Systems Biology 3:65-87).
As SigA is the housekeeping sigma factor for ''Synechocystis'' sp. PCC 6803, this promoter is likely constitutive, with a moderate level of expression (this is pending experimental verification).
false
false
_518_
0
6788
9
It's complicated
false
none
false
Cody Tramp
annotation2076278
1
sigA promoter
range2076278
1
1
42
annotation2076280
1
-10 box
range2076280
1
27
32
annotation2076279
1
-35 box
range2076279
1
6
11
BBa_K390006_sequence
1
caactttgactgaccagctaattttgtacacgacttaggagt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z