LOCUS       BBa_K390031              962 bp    DNA     linear       15-Jun-2024
DEFINITION  sigA promoter, 5' UTR and native RBS, and GFP
ACCESSION   BBa_K390031
VERSION     BBa_K390031.1  
FEATURES             Location/Qualifiers
     promoter        1..42
                     /label=sigA promoter
     misc_feature    6..11
                     /label=-35 box
     misc_feature    27..32
                     /label=-10 box
     promoter        1..42
                     /label=BBa_K390006
     misc_feature    51..99
                     /label=5' UTR with native RBS
     RBS             85..88
                     /label=SDS core consensus
     RBS             83..92
                     /label=native RBS
     RBS             51..99
                     /label=BBa_K390001
     misc_feature    106..108
                     /label=Start
     CDS             106..825
                     /label=GFP Coding Region
     CDS             106..825
                     /label=BBa_K208000
     stem_loop       845..888
                     /label=stem_loop
     misc_feature    834..913
                     /label=BBa_B0010
     terminator      834..913
                     /label=BBa_B0010
     stem_loop       929..948
                     /label=T7 TE
     misc_feature    955..955
                     /label=stop
     polyA_site      949..962
                     /label=polya
     misc_feature    922..962
                     /label=BBa_B0012
     terminator      922..962
                     /label=BBa_B0012
     misc_feature    106..962
                     /label=BBa_K208014
ORIGIN
        1 caactttgac tgaccagcta attttgtaca cgacttagga gttactagag agtcagttcc
       61 aatctgaaca tcgacaaata cataaggaat tataaccaat actagatggc tagcaaagga
      121 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
      181 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcta catacggaaa gcttaccctt
      241 aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt cactactttc
      301 tcttatggtg ttcaatgctt ttcccgttat ccggatcata tgaaacggca tgactttttc
      361 aagagtgcca tgcccgaagg ttatgtacag gaacgcacta tatctttcaa agatgacggg
      421 aactacaaga cgcgtgctga agtcaagttt gaaggtgata cccttgttaa tcgtatcgag
      481 ttaaaaggta ttgattttaa agaagatgga aacattctcg gacacaaact cgagtacaac
      541 tataactcac acaatgtata catcacggca gacaaacaaa agaatggaat caaagctaac
      601 ttcaaaattc gccacaacat tgaagatgga tccgttcaac tagcagacca ttatcaacaa
      661 aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtcgacacaa
      721 tctgcccttt cgaaagatcc caacgaaaag cgtgaccaca tggtccttct tgagtttgta
      781 actgctgctg ggattacaca tggcatggat gagctctaca aataatacta gagccaggca
      841 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
      901 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
      961 ta
//