LOCUS       BBa_K390035              958 bp    DNA     linear       26-Nov-2024
DEFINITION  hspA promoter, 5' UTR and native RBS, and GFP
ACCESSION   BBa_K390035
VERSION     BBa_K390035.1  
FEATURES             Location/Qualifiers
     misc_feature    6..11
                     /label=-35 box
     promoter        1..38
                     /label=hspA promoter
     misc_feature    27..32
                     /label=-10 box
     promoter        1..38
                     /label=BBa_K390010
     misc_feature    47..95
                     /label=5' UTR with native RBS
     RBS             81..84
                     /label=SDS core consensus
     RBS             79..88
                     /label=native RBS
     RBS             47..95
                     /label=BBa_K390001
     misc_feature    102..104
                     /label=Start
     CDS             102..821
                     /label=GFP Coding Region
     CDS             102..821
                     /label=BBa_K208000
     stem_loop       841..884
                     /label=stem_loop
     misc_feature    830..909
                     /label=BBa_B0010
     terminator      830..909
                     /label=BBa_B0010
     stem_loop       925..944
                     /label=T7 TE
     misc_feature    951..951
                     /label=stop
     polyA_site      945..958
                     /label=polya
     misc_feature    918..958
                     /label=BBa_B0012
     terminator      918..958
                     /label=BBa_B0012
     misc_feature    102..958
                     /label=BBa_K208014
ORIGIN
        1 cgaatctaac ctggaagggg aaattttaag atagaaccta ctagagagtc agttccaatc
       61 tgaacatcga caaatacata aggaattata accaatacta gatggctagc aaaggagaag
      121 aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca
      181 aattttctgt cagtggagag ggtgaaggtg atgctacata cggaaagctt acccttaaat
      241 ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact actttctctt
      301 atggtgttca atgcttttcc cgttatccgg atcatatgaa acggcatgac tttttcaaga
      361 gtgccatgcc cgaaggttat gtacaggaac gcactatatc tttcaaagat gacgggaact
      421 acaagacgcg tgctgaagtc aagtttgaag gtgataccct tgttaatcgt atcgagttaa
      481 aaggtattga ttttaaagaa gatggaaaca ttctcggaca caaactcgag tacaactata
      541 actcacacaa tgtatacatc acggcagaca aacaaaagaa tggaatcaaa gctaacttca
      601 aaattcgcca caacattgaa gatggatccg ttcaactagc agaccattat caacaaaata
      661 ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcg acacaatctg
      721 ccctttcgaa agatcccaac gaaaagcgtg accacatggt ccttcttgag tttgtaactg
      781 ctgctgggat tacacatggc atggatgagc tctacaaata atactagagc caggcatcaa
      841 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
      901 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//