BBa_K208014
1
BBa_K208014
GFP (Silver Fusion)/Double Terminator
2009-10-19T11:00:00Z
2015-05-08T01:11:24Z
Composite of GFP with BioBrick Terminator
GFP with Terminator
false
true
_310_
0
3473
9
It's complicated
false
Silver Fusion
false
USU iGEM 2009
component2056813
1
BBa_B0012
component2056811
1
BBa_B0010
component2056810
1
BBa_K208000
annotation2056813
1
BBa_B0012
range2056813
1
817
857
annotation2056810
1
BBa_K208000
range2056810
1
1
720
annotation2056811
1
BBa_B0010
range2056811
1
729
808
BBa_K390002
1
BBa_K390002
5'UTR and consensus RBS from psbA2 in Synechocystis sp. PCC 6803
2010-07-31T11:00:00Z
2015-05-08T01:12:19Z
Based on genomic sequence in Synechocystis sp. PCC 6803. Made from annealing of two oligionucleotides that contained the mutations to the consensus RBS.
This is the 5'UTR and RBS region from gene psbA2 in Synechocystis sp. PCC 6803. It covers the entire region from the end of the promoter (as reported in Imamura S and Asayama M. 2009. Sigma Factors for Cyanobacterial Transcription. Gene Regulation and Systems Biology 3: 65-87) to immediately before the start codon of the psbA2 gene.
The RBS region has been mutated from the native RBS "taaggaatta" to the consensus RBS "aaaggaggtt" for Synechocystis sp. PCC 6803.
This part can serve as a general RBS for Synechocystis sp. PCC 6803, until a library of RBS strengths in PCC 6803 is assembled. The consensus RBS was determined by aligning the 9 nucleotides on the 3' end sequence of the 16S rRNA to the 20 nucleotides preceding the start codon of every gene in PCC 6803. The most frequently represented nucleotide at each location along the alignment was considered to be the consensus. (Note: the alignment was done in relation to the 16S rRNA sequence, and not the start codon, as the spacing between RBS and the start codon varied considerably from gene to gene).
false
false
_518_
0
6788
9
It's complicated
false
none
false
Cody Tramp
annotation2076211
1
5' UTR with consensus RBS
range2076211
1
1
49
annotation2076289
1
t > a
range2076289
1
33
33
annotation2076212
1
consensus RBS
range2076212
1
33
42
annotation2076269
1
SDS core consensus
range2076269
1
35
40
annotation2076291
1
a > t
range2076291
1
42
42
annotation2076290
1
at > gg
range2076290
1
39
40
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1690
1
polya
range1690
1
28
41
annotation1687
1
stop
range1687
1
34
34
annotation1686
1
T7 TE
range1686
1
8
27
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_K390009
1
BBa_K390009
sigF Type III promoter from Synechocystis sp. PCC 6803
2010-07-31T11:00:00Z
2015-05-08T01:12:19Z
Source was genomic sequence, but was constructed by annealing two oligonucleotides together.
This is the promoter for the ''sig''F sigma factor in ''Synechocystis'' sp. PCC 6803. It is self-regulated by the SigF protein. The protein is present at levels below detectable limits, but is still present in enough quantity to control type III promoter expression (Imamura S, and Asayama M. 2009. Sigma Factors for Cyanobacterial Transcription. Gene Regulation and Systems Biology 3:65-87). This could potentially be a low-level constitutive promoter.
false
false
_518_
0
6788
9
It's complicated
false
none
false
Cody Tramp
annotation2076287
1
-32 box
range2076287
1
6
10
annotation2076286
1
sigF promoter
range2076286
1
1
37
annotation2076288
1
-10 box
range2076288
1
25
29
BBa_K208000
1
BBa_K208000
GFP
2009-10-07T11:00:00Z
2015-05-08T01:11:24Z
plasmid
GFP
false
false
_310_
0
2425
303
It's complicated
true
Mutate PstI sites
false
Elisabeth Linton
annotation2062217
1
GFP Coding Region
range2062217
1
1
720
annotation2062216
1
Start
range2062216
1
1
3
BBa_K390042
1
BBa_K390042
sigF promoter, 5' UTR and consensus RBS, and GFP
2010-08-01T11:00:00Z
2015-05-08T01:12:20Z
(coming soon)
(coming soon)
false
false
_518_
0
6788
9
Not in stock
false
(coming soon)
false
Cody Tramp
component2111285
1
BBa_K390009
component2111300
1
BBa_K208014
component2111292
1
BBa_K390002
annotation2111285
1
BBa_K390009
range2111285
1
1
37
annotation2111300
1
BBa_K208014
range2111300
1
101
957
annotation2111292
1
BBa_K390002
range2111292
1
46
94
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K390002_sequence
1
agtcagttccaatctgaacatcgacaaatacaaaaggaggtttaaccaa
BBa_K390009_sequence
1
tgatgtaggcagagtccaaccatcggtaaagttgatt
BBa_K390042_sequence
1
tgatgtaggcagagtccaaccatcggtaaagttgatttactagagagtcagttccaatctgaacatcgacaaatacaaaaggaggtttaaccaatactagatggctagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaagcttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttctcttatggtgttcaatgcttttcccgttatccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactcgagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggatccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K208014_sequence
1
atggctagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaagcttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttctcttatggtgttcaatgcttttcccgttatccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactcgagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggatccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K208000_sequence
1
atggctagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaagcttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttctcttatggtgttcaatgcttttcccgttatccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactcgagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggatccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaataa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z