LOCUS       BBa_K390044              968 bp    DNA     linear       15-Jun-2024
DEFINITION  glnBP2 promoter, 5' UTR and consensus RBS, and GFP
ACCESSION   BBa_K390044
VERSION     BBa_K390044.1  
FEATURES             Location/Qualifiers
     promoter        1..48
                     /label=glnBP2 promoter
     misc_feature    1..14
                     /label=NctA binding site
     misc_feature    38..43
                     /label=-10 box
     promoter        1..48
                     /label=BBa_K390011
     misc_feature    57..105
                     /label=5' UTR with consensus RBS
     RBS             89..98
                     /label=consensus RBS
     RBS             91..96
                     /label=SDS core consensus
     misc_feature    89..89
                     /label=t > a
     misc_feature    95..96
                     /label=at > gg
     misc_feature    98..98
                     /label=a > t
     RBS             57..105
                     /label=BBa_K390002
     misc_feature    112..114
                     /label=Start
     CDS             112..831
                     /label=GFP Coding Region
     CDS             112..831
                     /label=BBa_K208000
     stem_loop       851..894
                     /label=stem_loop
     misc_feature    840..919
                     /label=BBa_B0010
     terminator      840..919
                     /label=BBa_B0010
     stem_loop       935..954
                     /label=T7 TE
     misc_feature    961..961
                     /label=stop
     polyA_site      955..968
                     /label=polya
     misc_feature    928..968
                     /label=BBa_B0012
     terminator      928..968
                     /label=BBa_B0012
     misc_feature    112..968
                     /label=BBa_K208014
ORIGIN
        1 gtactgattt ttacaaaaaa acttttggag gacatgttaa aagtgtctta ctagagagtc
       61 agttccaatc tgaacatcga caaatacaaa aggaggttta accaatacta gatggctagc
      121 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt
      181 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgctacata cggaaagctt
      241 acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact
      301 actttctctt atggtgttca atgcttttcc cgttatccgg atcatatgaa acggcatgac
      361 tttttcaaga gtgccatgcc cgaaggttat gtacaggaac gcactatatc tttcaaagat
      421 gacgggaact acaagacgcg tgctgaagtc aagtttgaag gtgataccct tgttaatcgt
      481 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttctcggaca caaactcgag
      541 tacaactata actcacacaa tgtatacatc acggcagaca aacaaaagaa tggaatcaaa
      601 gctaacttca aaattcgcca caacattgaa gatggatccg ttcaactagc agaccattat
      661 caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcg
      721 acacaatctg ccctttcgaa agatcccaac gaaaagcgtg accacatggt ccttcttgag
      781 tttgtaactg ctgctgggat tacacatggc atggatgagc tctacaaata atactagagc
      841 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
      901 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
      961 cgtttata
//