LOCUS BBa_K390044 968 bp DNA linear 22-Nov-2024 DEFINITION glnBP2 promoter, 5' UTR and consensus RBS, and GFP ACCESSION BBa_K390044 VERSION BBa_K390044.1 FEATURES Location/Qualifiers promoter 1..48 /label=glnBP2 promoter misc_feature 1..14 /label=NctA binding site misc_feature 38..43 /label=-10 box promoter 1..48 /label=BBa_K390011 misc_feature 57..105 /label=5' UTR with consensus RBS RBS 89..98 /label=consensus RBS RBS 91..96 /label=SDS core consensus misc_feature 89..89 /label=t > a misc_feature 95..96 /label=at > gg misc_feature 98..98 /label=a > t RBS 57..105 /label=BBa_K390002 misc_feature 112..114 /label=Start CDS 112..831 /label=GFP Coding Region CDS 112..831 /label=BBa_K208000 stem_loop 851..894 /label=stem_loop misc_feature 840..919 /label=BBa_B0010 terminator 840..919 /label=BBa_B0010 stem_loop 935..954 /label=T7 TE misc_feature 961..961 /label=stop polyA_site 955..968 /label=polya misc_feature 928..968 /label=BBa_B0012 terminator 928..968 /label=BBa_B0012 misc_feature 112..968 /label=BBa_K208014 ORIGIN 1 gtactgattt ttacaaaaaa acttttggag gacatgttaa aagtgtctta ctagagagtc 61 agttccaatc tgaacatcga caaatacaaa aggaggttta accaatacta gatggctagc 121 aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt 181 aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgctacata cggaaagctt 241 acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact 301 actttctctt atggtgttca atgcttttcc cgttatccgg atcatatgaa acggcatgac 361 tttttcaaga gtgccatgcc cgaaggttat gtacaggaac gcactatatc tttcaaagat 421 gacgggaact acaagacgcg tgctgaagtc aagtttgaag gtgataccct tgttaatcgt 481 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttctcggaca caaactcgag 541 tacaactata actcacacaa tgtatacatc acggcagaca aacaaaagaa tggaatcaaa 601 gctaacttca aaattcgcca caacattgaa gatggatccg ttcaactagc agaccattat 661 caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcg 721 acacaatctg ccctttcgaa agatcccaac gaaaagcgtg accacatggt ccttcttgag 781 tttgtaactg ctgctgggat tacacatggc atggatgagc tctacaaata atactagagc 841 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 901 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg 961 cgtttata //