##gff-version 3
##sequence-region BBa_K390045 1 957
BBa_K390045	.	engineered_region	101	957	.	+	0	ID=BBa_K208014;Name=BBa_K208014
BBa_K390045	.	CDS	101	820	.	+	0	ID=BBa_K208000;Name=BBa_K208000;Parent=BBa_K208014
BBa_K390045	.	CDS	101	820	.	+	0	ID=annotation2062217;Name=GFP Coding Region;Parent=BBa_K208000
BBa_K390045	.	start_codon	101	103	.	+	0	ID=annotation2062216;Name=Start;Parent=BBa_K208000
BBa_K390045	.	terminator	829	908	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K208014
BBa_K390045	.	stem_loop	840	883	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K390045	.	engineered_region	829	908	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K390045	.	terminator	917	957	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K208014
BBa_K390045	.	polyA_site	944	957	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K390045	.	stem_loop	924	943	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K390045	.	stop_codon	950	950	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K390045	.	engineered_region	917	957	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K390045	.	ribosome_entry_site	46	94	.	+	0	ID=BBa_K390002;Name=BBa_K390002
BBa_K390045	.	sequence_alteration	78	78	.	+	0	ID=annotation2076289;Name=t > a;Parent=BBa_K390002
BBa_K390045	.	sequence_alteration	84	85	.	+	0	ID=annotation2076290;Name=at > gg;Parent=BBa_K390002
BBa_K390045	.	ribosome_entry_site	78	87	.	+	0	ID=annotation2076212;Name=consensus RBS;Parent=BBa_K390002
BBa_K390045	.	sequence_feature	46	94	.	+	0	ID=annotation2076211;Name=5' UTR with consensus RBS;Parent=BBa_K390002
BBa_K390045	.	sequence_alteration	87	87	.	+	0	ID=annotation2076291;Name=a > t;Parent=BBa_K390002
BBa_K390045	.	ribosome_entry_site	80	85	.	+	0	ID=annotation2076269;Name=SDS core consensus;Parent=BBa_K390002
BBa_K390045	.	promoter	1	37	.	+	0	ID=BBa_K390012;Name=BBa_K390012
BBa_K390045	.	sequence_feature	5	9	.	+	0	ID=annotation2076298;Name=-32 box;Parent=BBa_K390012
BBa_K390045	.	sequence_feature	24	28	.	+	0	ID=annotation2076299;Name=-10 box;Parent=BBa_K390012
BBa_K390045	.	promoter	1	37	.	+	0	ID=annotation2076300;Name=pilA1 promoter;Parent=BBa_K390012
>BBa_K390045
gtgataggcaaatgagggcgatgggtaagttacagcttactagagagtcagttccaatctgaacatcgacaaatacaaa
aggaggtttaaccaatactagatggctagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattag
atggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaagcttacccttaa
atttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttctcttatggtgttcaatgcttt
tcccgttatccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcacta
tatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcga
gttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactcgagtacaactataactcacacaatgta
tacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggatccgttc
aactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgac
acaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggatt
acacatggcatggatgagctctacaaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgg
gcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttct
gcgtttata