LOCUS       BBa_K390046             1154 bp    DNA     linear       23-Dec-2024
DEFINITION  cpcBA operon promoter, 5'UTR, and RBS with GFP
ACCESSION   BBa_K390046
VERSION     BBa_K390046.1  
FEATURES             Location/Qualifiers
     promoter        1..39
                     /label=cpcBA operon promoter
     RBS             278..285
                     /label=putative rbs
     misc_feature    3..8
                     /label=-35 box
     misc_feature    27..32
                     /label=-10 box
     misc_feature    40..40
                     /label=+1 Transcription Start Point
     promoter        1..291
                     /label=BBa_K390013
     misc_feature    298..300
                     /label=Start
     CDS             298..1017
                     /label=GFP Coding Region
     CDS             298..1017
                     /label=BBa_K208000
     stem_loop       1037..1080
                     /label=stem_loop
     misc_feature    1026..1105
                     /label=BBa_B0010
     terminator      1026..1105
                     /label=BBa_B0010
     stem_loop       1121..1140
                     /label=T7 TE
     misc_feature    1147..1147
                     /label=stop
     polyA_site      1141..1154
                     /label=polya
     misc_feature    1114..1154
                     /label=BBa_B0012
     terminator      1114..1154
                     /label=BBa_B0012
     misc_feature    298..1154
                     /label=BBa_K208014
ORIGIN
        1 aacagacata agtcccatca ccgttgtata aagttaactg tgggattgca aaagcattca
       61 agcctaggcg ctgagctgtt tgagcatccc ggtggccctt gtcgctgcct ccgtgtttct
      121 ccctggattt atttaggtaa tatctctcat aaatccccgg gtagttaacg aaagttaatg
      181 gagatcagta acaataactc tagggtcatt actttggact ccctcagttt atccggggga
      241 attgtgttta agaaaatccc aactcataaa gtcaagtagg agattaattc atactagatg
      301 gctagcaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga attagatggt
      361 gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc tacatacgga
      421 aagcttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg gccaacactt
      481 gtcactactt tctcttatgg tgttcaatgc ttttcccgtt atccggatca tatgaaacgg
      541 catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaacgcac tatatctttc
      601 aaagatgacg ggaactacaa gacgcgtgct gaagtcaagt ttgaaggtga tacccttgtt
      661 aatcgtatcg agttaaaagg tattgatttt aaagaagatg gaaacattct cggacacaaa
      721 ctcgagtaca actataactc acacaatgta tacatcacgg cagacaaaca aaagaatgga
      781 atcaaagcta acttcaaaat tcgccacaac attgaagatg gatccgttca actagcagac
      841 cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga caaccattac
      901 ctgtcgacac aatctgccct ttcgaaagat cccaacgaaa agcgtgacca catggtcctt
      961 cttgagtttg taactgctgc tgggattaca catggcatgg atgagctcta caaataatac
     1021 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     1081 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
     1141 tttctgcgtt tata
//