LOCUS       BBa_K390048             1042 bp    DNA     linear       23-Dec-2024
DEFINITION  petBD operon promoter, 5'UTR, and RBS with GFP
ACCESSION   BBa_K390048
VERSION     BBa_K390048.1  
FEATURES             Location/Qualifiers
     promoter        1..42
                     /label=petBD operon promoter
     RBS             162..171
                     /label=putative RBS
     misc_feature    4..9
                     /label=-35 box
     misc_feature    27..32
                     /label=-10 box
     misc_feature    43..43
                     /label=+1 Transcription Start Point
     promoter        1..179
                     /label=BBa_K390015
     misc_feature    186..188
                     /label=Start
     CDS             186..905
                     /label=GFP Coding Region
     CDS             186..905
                     /label=BBa_K208000
     stem_loop       925..968
                     /label=stem_loop
     misc_feature    914..993
                     /label=BBa_B0010
     terminator      914..993
                     /label=BBa_B0010
     stem_loop       1009..1028
                     /label=T7 TE
     misc_feature    1035..1035
                     /label=stop
     polyA_site      1029..1042
                     /label=polya
     misc_feature    1002..1042
                     /label=BBa_B0012
     terminator      1002..1042
                     /label=BBa_B0012
     misc_feature    186..1042
                     /label=BBa_K208014
ORIGIN
        1 cctttcccca gggcttgaag ctgtgttaca ttttgtgatg agtagttctc atttttgcca
       61 agtttgggag cttttccacc cttggtcaaa ccgtcatagt acgctgatta tatgcgattt
      121 tacggctccg ccggagtcaa ccccctccat ccctgatcct taagagaagt ctagcttcat
      181 actagatggc tagcaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat
      241 tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcta
      301 catacggaaa gcttaccctt aaatttattt gcactactgg aaaactacct gttccatggc
      361 caacacttgt cactactttc tcttatggtg ttcaatgctt ttcccgttat ccggatcata
      421 tgaaacggca tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaacgcacta
      481 tatctttcaa agatgacggg aactacaaga cgcgtgctga agtcaagttt gaaggtgata
      541 cccttgttaa tcgtatcgag ttaaaaggta ttgattttaa agaagatgga aacattctcg
      601 gacacaaact cgagtacaac tataactcac acaatgtata catcacggca gacaaacaaa
      661 agaatggaat caaagctaac ttcaaaattc gccacaacat tgaagatgga tccgttcaac
      721 tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca
      781 accattacct gtcgacacaa tctgcccttt cgaaagatcc caacgaaaag cgtgaccaca
      841 tggtccttct tgagtttgta actgctgctg ggattacaca tggcatggat gagctctaca
      901 aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
      961 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
     1021 ggtgggcctt tctgcgttta ta
//