LOCUS       BBa_K390049             1056 bp    DNA     linear       27-Dec-2024
DEFINITION  psaAB operon promoter, 5'UTR, and RBS with GFP
ACCESSION   BBa_K390049
VERSION     BBa_K390049.1  
FEATURES             Location/Qualifiers
     promoter        9..47
                     /label=psaAB operon promoter
     misc_feature    48..50
                     /label=+1 Transcription Start Point(s)
     misc_feature    177..185
                     /label=rbs region (non exact)
     misc_feature    13..18
                     /label=-35 box
     misc_feature    35..40
                     /label=-10 box
     promoter        1..193
                     /label=BBa_K390016
     misc_feature    200..202
                     /label=Start
     CDS             200..919
                     /label=GFP Coding Region
     CDS             200..919
                     /label=BBa_K208000
     stem_loop       939..982
                     /label=stem_loop
     misc_feature    928..1007
                     /label=BBa_B0010
     terminator      928..1007
                     /label=BBa_B0010
     stem_loop       1023..1042
                     /label=T7 TE
     misc_feature    1049..1049
                     /label=stop
     polyA_site      1043..1056
                     /label=polya
     misc_feature    1016..1056
                     /label=BBa_B0012
     terminator      1016..1056
                     /label=BBa_B0012
     misc_feature    200..1056
                     /label=BBa_K208014
ORIGIN
        1 gttacgcagg tcttgcctag ggggggggag gccgtattat cttctagtga tgtttgctga
       61 aaacgcctat ctgtgcaagg tttaacatcg ttattatgaa gcgaaaacta attccctttt
      121 ttacgcttcc tctattacac tattctgcat aggaaaccct taatagttca ttgtcgagcg
      181 aggagaaccc tgctactaga tggctagcaa aggagaagaa cttttcactg gagttgtccc
      241 aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg
      301 tgaaggtgat gctacatacg gaaagcttac ccttaaattt atttgcacta ctggaaaact
      361 acctgttcca tggccaacac ttgtcactac tttctcttat ggtgttcaat gcttttcccg
      421 ttatccggat catatgaaac ggcatgactt tttcaagagt gccatgcccg aaggttatgt
      481 acaggaacgc actatatctt tcaaagatga cgggaactac aagacgcgtg ctgaagtcaa
      541 gtttgaaggt gatacccttg ttaatcgtat cgagttaaaa ggtattgatt ttaaagaaga
      601 tggaaacatt ctcggacaca aactcgagta caactataac tcacacaatg tatacatcac
      661 ggcagacaaa caaaagaatg gaatcaaagc taacttcaaa attcgccaca acattgaaga
      721 tggatccgtt caactagcag accattatca acaaaatact ccaattggcg atggccctgt
      781 ccttttacca gacaaccatt acctgtcgac acaatctgcc ctttcgaaag atcccaacga
      841 aaagcgtgac cacatggtcc ttcttgagtt tgtaactgct gctgggatta cacatggcat
      901 ggatgagctc tacaaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga
      961 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca
     1021 ctggctcacc ttcgggtggg cctttctgcg tttata
//