LOCUS       BBa_K390051              924 bp    DNA     linear       22-Dec-2024
DEFINITION  sigA promoter, 5'UTR, and RBS with GFP
ACCESSION   BBa_K390051
VERSION     BBa_K390051.1  
FEATURES             Location/Qualifiers
     promoter        4..45
                     /label=sigA promoter
     RBS             46..55
                     /label=putative rbs
     misc_feature    46..46
                     /label=+1 Transcription Start Point
     misc_feature    9..14
                     /label=-35 box
     misc_feature    30..35
                     /label=-10 box
     promoter        1..61
                     /label=BBa_K390018
     misc_feature    68..70
                     /label=Start
     CDS             68..787
                     /label=GFP Coding Region
     CDS             68..787
                     /label=BBa_K208000
     stem_loop       807..850
                     /label=stem_loop
     misc_feature    796..875
                     /label=BBa_B0010
     terminator      796..875
                     /label=BBa_B0010
     stem_loop       891..910
                     /label=T7 TE
     misc_feature    917..917
                     /label=stop
     polyA_site      911..924
                     /label=polya
     misc_feature    884..924
                     /label=BBa_B0012
     terminator      884..924
                     /label=BBa_B0012
     misc_feature    68..924
                     /label=BBa_K208014
ORIGIN
        1 cctcaacttt gactgaccag ctaattttgt acacgactta ggagttaacg aggaaaacgg
       61 ctactagatg gctagcaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga
      121 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc
      181 tacatacgga aagcttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg
      241 gccaacactt gtcactactt tctcttatgg tgttcaatgc ttttcccgtt atccggatca
      301 tatgaaacgg catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaacgcac
      361 tatatctttc aaagatgacg ggaactacaa gacgcgtgct gaagtcaagt ttgaaggtga
      421 tacccttgtt aatcgtatcg agttaaaagg tattgatttt aaagaagatg gaaacattct
      481 cggacacaaa ctcgagtaca actataactc acacaatgta tacatcacgg cagacaaaca
      541 aaagaatgga atcaaagcta acttcaaaat tcgccacaac attgaagatg gatccgttca
      601 actagcagac cattatcaac aaaatactcc aattggcgat ggccctgtcc ttttaccaga
      661 caaccattac ctgtcgacac aatctgccct ttcgaaagat cccaacgaaa agcgtgacca
      721 catggtcctt cttgagtttg taactgctgc tgggattaca catggcatgg atgagctcta
      781 caaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
      841 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
      901 cgggtgggcc tttctgcgtt tata
//