LOCUS       BBa_K390053             1213 bp    DNA     linear       22-Nov-2024
DEFINITION  lrtA promoter, 5'UTR, and RBS with GFP
ACCESSION   BBa_K390053
VERSION     BBa_K390053.1  
FEATURES             Location/Qualifiers
     misc_feature    342..347
                     /label=illegal EcoRI site
     promoter        1..38
                     /label=lrtA promoter
     RBS             336..345
                     /label=putative RBS
     misc_feature    27..32
                     /label=-10 box
     promoter        1..350
                     /label=BBa_K390020
     misc_feature    357..359
                     /label=Start
     CDS             357..1076
                     /label=GFP Coding Region
     CDS             357..1076
                     /label=BBa_K208000
     stem_loop       1096..1139
                     /label=stem_loop
     misc_feature    1085..1164
                     /label=BBa_B0010
     terminator      1085..1164
                     /label=BBa_B0010
     stem_loop       1180..1199
                     /label=T7 TE
     misc_feature    1206..1206
                     /label=stop
     polyA_site      1200..1213
                     /label=polya
     misc_feature    1173..1213
                     /label=BBa_B0012
     terminator      1173..1213
                     /label=BBa_B0012
     misc_feature    357..1213
                     /label=BBa_K208014
ORIGIN
        1 attaggtctt attcaataca tagtgctaat ctgaagatag tcttaggagt taattattta
       61 ccaccacaat tttctggaaa actttacctc taccctaggg atgattaaaa gtaaactaga
      121 gaataacaag gttgggttta taattcatca ccaagctcaa atttatggtg ttttttcaat
      181 gatccatgct tttgatatct ttagcagaaa ggcattttaa gtaatgattc cacctcactg
      241 tttctcggaa aaattgccca atctaactta gtttttataa cttaagttta gatctgcgga
      301 aaaccaacca ttgctcattt tttattaatt ttacgaaggg agaattcagt tactagatgg
      361 ctagcaaagg agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg
      421 atgttaatgg gcacaaattt tctgtcagtg gagagggtga aggtgatgct acatacggaa
      481 agcttaccct taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg
      541 tcactacttt ctcttatggt gttcaatgct tttcccgtta tccggatcat atgaaacggc
      601 atgacttttt caagagtgcc atgcccgaag gttatgtaca ggaacgcact atatctttca
      661 aagatgacgg gaactacaag acgcgtgctg aagtcaagtt tgaaggtgat acccttgtta
      721 atcgtatcga gttaaaaggt attgatttta aagaagatgg aaacattctc ggacacaaac
      781 tcgagtacaa ctataactca cacaatgtat acatcacggc agacaaacaa aagaatggaa
      841 tcaaagctaa cttcaaaatt cgccacaaca ttgaagatgg atccgttcaa ctagcagacc
      901 attatcaaca aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc
      961 tgtcgacaca atctgccctt tcgaaagatc ccaacgaaaa gcgtgaccac atggtccttc
     1021 ttgagtttgt aactgctgct gggattacac atggcatgga tgagctctac aaataatact
     1081 agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc
     1141 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
     1201 ttctgcgttt ata
//