BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_K208000
1
BBa_K208000
GFP
2009-10-07T11:00:00Z
2015-05-08T01:11:24Z
plasmid
GFP
false
false
_310_
0
2425
303
It's complicated
true
Mutate PstI sites
false
Elisabeth Linton
annotation2062216
1
Start
range2062216
1
1
3
annotation2062217
1
GFP Coding Region
range2062217
1
1
720
BBa_K390053
1
BBa_K390053
lrtA promoter, 5'UTR, and RBS with GFP
2010-08-02T11:00:00Z
2015-05-08T01:12:20Z
(coming soon)
(coming soon)
false
false
_518_
0
6788
9
Not in stock
false
(coming soon)
false
Cody Tramp
component2111440
1
BBa_K208014
component2111432
1
BBa_K390020
annotation2111432
1
BBa_K390020
range2111432
1
1
350
annotation2111440
1
BBa_K208014
range2111440
1
357
1213
BBa_K390020
1
BBa_K390020
lrtA promoter, 5'UTR, and RBS
2010-08-01T11:00:00Z
2015-05-08T01:12:19Z
(coming soon)
(coming soon)
false
false
_518_
0
6788
9
Not in stock
false
(coming soon)
false
Cody Tramp
annotation2076621
1
-10 box
range2076621
1
27
32
annotation2076618
1
illegal EcoRI site
range2076618
1
342
347
annotation2076620
1
putative RBS
range2076620
1
336
345
annotation2076619
1
lrtA promoter
range2076619
1
1
38
BBa_K208014
1
BBa_K208014
GFP (Silver Fusion)/Double Terminator
2009-10-19T11:00:00Z
2015-05-08T01:11:24Z
Composite of GFP with BioBrick Terminator
GFP with Terminator
false
true
_310_
0
3473
9
It's complicated
false
Silver Fusion
false
USU iGEM 2009
component2056813
1
BBa_B0012
component2056810
1
BBa_K208000
component2056811
1
BBa_B0010
annotation2056811
1
BBa_B0010
range2056811
1
729
808
annotation2056813
1
BBa_B0012
range2056813
1
817
857
annotation2056810
1
BBa_K208000
range2056810
1
1
720
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K390020_sequence
1
attaggtcttattcaatacatagtgctaatctgaagatagtcttaggagttaattatttaccaccacaattttctggaaaactttacctctaccctagggatgattaaaagtaaactagagaataacaaggttgggtttataattcatcaccaagctcaaatttatggtgttttttcaatgatccatgcttttgatatctttagcagaaaggcattttaagtaatgattccacctcactgtttctcggaaaaattgcccaatctaacttagtttttataacttaagtttagatctgcggaaaaccaaccattgctcattttttattaattttacgaagggagaattcagt
BBa_K208014_sequence
1
atggctagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaagcttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttctcttatggtgttcaatgcttttcccgttatccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactcgagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggatccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K208000_sequence
1
atggctagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaagcttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttctcttatggtgttcaatgcttttcccgttatccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactcgagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggatccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaataa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K390053_sequence
1
attaggtcttattcaatacatagtgctaatctgaagatagtcttaggagttaattatttaccaccacaattttctggaaaactttacctctaccctagggatgattaaaagtaaactagagaataacaaggttgggtttataattcatcaccaagctcaaatttatggtgttttttcaatgatccatgcttttgatatctttagcagaaaggcattttaagtaatgattccacctcactgtttctcggaaaaattgcccaatctaacttagtttttataacttaagtttagatctgcggaaaaccaaccattgctcattttttattaattttacgaagggagaattcagttactagatggctagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaagcttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttctcttatggtgttcaatgcttttcccgttatccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactcgagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggatccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z