LOCUS       BBa_K390055              963 bp    DNA     linear       29-Nov-2024
DEFINITION  sigB promoter, 5'UTR, and RBS with GFP
ACCESSION   BBa_K390055
VERSION     BBa_K390055.1  
FEATURES             Location/Qualifiers
     promoter        1..100
                     /label=BBa_K390022
     misc_feature    107..109
                     /label=Start
     CDS             107..826
                     /label=GFP Coding Region
     CDS             107..826
                     /label=BBa_K208000
     stem_loop       846..889
                     /label=stem_loop
     misc_feature    835..914
                     /label=BBa_B0010
     terminator      835..914
                     /label=BBa_B0010
     stem_loop       930..949
                     /label=T7 TE
     misc_feature    956..956
                     /label=stop
     polyA_site      950..963
                     /label=polya
     misc_feature    923..963
                     /label=BBa_B0012
     terminator      923..963
                     /label=BBa_B0012
     misc_feature    107..963
                     /label=BBa_K208014
ORIGIN
        1 ctaaaggccc actcgttagg acacggtgta aaaaaaattg acgactgcac taccctattc
       61 tccaccatca atgacttagt ctaagacatt tttgggaaag tactagatgg ctagcaaagg
      121 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg
      181 gcacaaattt tctgtcagtg gagagggtga aggtgatgct acatacggaa agcttaccct
      241 taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt
      301 ctcttatggt gttcaatgct tttcccgtta tccggatcat atgaaacggc atgacttttt
      361 caagagtgcc atgcccgaag gttatgtaca ggaacgcact atatctttca aagatgacgg
      421 gaactacaag acgcgtgctg aagtcaagtt tgaaggtgat acccttgtta atcgtatcga
      481 gttaaaaggt attgatttta aagaagatgg aaacattctc ggacacaaac tcgagtacaa
      541 ctataactca cacaatgtat acatcacggc agacaaacaa aagaatggaa tcaaagctaa
      601 cttcaaaatt cgccacaaca ttgaagatgg atccgttcaa ctagcagacc attatcaaca
      661 aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc tgtcgacaca
      721 atctgccctt tcgaaagatc ccaacgaaaa gcgtgaccac atggtccttc ttgagtttgt
      781 aactgctgct gggattacac atggcatgga tgagctctac aaataatact agagccaggc
      841 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
      901 cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
      961 ata
//