LOCUS       BBa_K390057              955 bp    DNA     linear       26-Nov-2024
DEFINITION  pilA1 promoter, 5'UTR, and RBS with GFP
ACCESSION   BBa_K390057
VERSION     BBa_K390057.1  
FEATURES             Location/Qualifiers
     promoter        1..38
                     /label=pilA1 promoter
     misc_feature    5..9
                     /label=-32 box
     misc_feature    25..29
                     /label=-12 box
     promoter        1..92
                     /label=BBa_K390024
     misc_feature    99..101
                     /label=Start
     CDS             99..818
                     /label=GFP Coding Region
     CDS             99..818
                     /label=BBa_K208000
     stem_loop       838..881
                     /label=stem_loop
     misc_feature    827..906
                     /label=BBa_B0010
     terminator      827..906
                     /label=BBa_B0010
     stem_loop       922..941
                     /label=T7 TE
     misc_feature    948..948
                     /label=stop
     polyA_site      942..955
                     /label=polya
     misc_feature    915..955
                     /label=BBa_B0012
     terminator      915..955
                     /label=BBa_B0012
     misc_feature    99..955
                     /label=BBa_K208014
ORIGIN
        1 gtgataggca aatgaggggc gatgggtaag ttacagctag aagggttccg accagttatt
       61 taaatttccc ctacagaagg aagaagacaa tctactagat ggctagcaaa ggagaagaac
      121 ttttcactgg agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat
      181 tttctgtcag tggagagggt gaaggtgatg ctacatacgg aaagcttacc cttaaattta
      241 tttgcactac tggaaaacta cctgttccat ggccaacact tgtcactact ttctcttatg
      301 gtgttcaatg cttttcccgt tatccggatc atatgaaacg gcatgacttt ttcaagagtg
      361 ccatgcccga aggttatgta caggaacgca ctatatcttt caaagatgac gggaactaca
      421 agacgcgtgc tgaagtcaag tttgaaggtg atacccttgt taatcgtatc gagttaaaag
      481 gtattgattt taaagaagat ggaaacattc tcggacacaa actcgagtac aactataact
      541 cacacaatgt atacatcacg gcagacaaac aaaagaatgg aatcaaagct aacttcaaaa
      601 ttcgccacaa cattgaagat ggatccgttc aactagcaga ccattatcaa caaaatactc
      661 caattggcga tggccctgtc cttttaccag acaaccatta cctgtcgaca caatctgccc
      721 tttcgaaaga tcccaacgaa aagcgtgacc acatggtcct tcttgagttt gtaactgctg
      781 ctgggattac acatggcatg gatgagctct acaaataata ctagagccag gcatcaaata
      841 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
      901 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//