LOCUS       BBa_K390058              941 bp    DNA     linear       16-Feb-2025
DEFINITION  pixJI promoter, 5'UTR, and RBS with GFP
ACCESSION   BBa_K390058
VERSION     BBa_K390058.1  
FEATURES             Location/Qualifiers
     promoter        1..46
                     /label=pixJI promoter
     misc_feature    5..9
                     /label=-32 box
     misc_feature    25..29
                     /label=-12 box
     promoter        1..78
                     /label=BBa_K390025
     misc_feature    85..87
                     /label=Start
     CDS             85..804
                     /label=GFP Coding Region
     CDS             85..804
                     /label=BBa_K208000
     stem_loop       824..867
                     /label=stem_loop
     misc_feature    813..892
                     /label=BBa_B0010
     terminator      813..892
                     /label=BBa_B0010
     stem_loop       908..927
                     /label=T7 TE
     misc_feature    934..934
                     /label=stop
     polyA_site      928..941
                     /label=polya
     misc_feature    901..941
                     /label=BBa_B0012
     terminator      901..941
                     /label=BBa_B0012
     misc_feature    85..941
                     /label=BBa_K208014
ORIGIN
        1 cccaagggcc attaccgtgc ttgaggtact gatgtccacc ctgccctacc catgttgcct
       61 aactggtaag ttttaaccta ctagatggct agcaaaggag aagaactttt cactggagtt
      121 gtcccaattc ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga
      181 gagggtgaag gtgatgctac atacggaaag cttaccctta aatttatttg cactactgga
      241 aaactacctg ttccatggcc aacacttgtc actactttct cttatggtgt tcaatgcttt
      301 tcccgttatc cggatcatat gaaacggcat gactttttca agagtgccat gcccgaaggt
      361 tatgtacagg aacgcactat atctttcaaa gatgacggga actacaagac gcgtgctgaa
      421 gtcaagtttg aaggtgatac ccttgttaat cgtatcgagt taaaaggtat tgattttaaa
      481 gaagatggaa acattctcgg acacaaactc gagtacaact ataactcaca caatgtatac
      541 atcacggcag acaaacaaaa gaatggaatc aaagctaact tcaaaattcg ccacaacatt
      601 gaagatggat ccgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc
      661 cctgtccttt taccagacaa ccattacctg tcgacacaat ctgccctttc gaaagatccc
      721 aacgaaaagc gtgaccacat ggtccttctt gagtttgtaa ctgctgctgg gattacacat
      781 ggcatggatg agctctacaa ataatactag agccaggcat caaataaaac gaaaggctca
      841 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
      901 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
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