LOCUS       BBa_K390059              922 bp    DNA     linear       26-Nov-2024
DEFINITION  sigF promoter, 5'UTR, and RBS with GFP
ACCESSION   BBa_K390059
VERSION     BBa_K390059.1  
FEATURES             Location/Qualifiers
     promoter        1..37
                     /label=sigF promoter
     misc_feature    6..10
                     /label=-32 box
     misc_feature    25..29
                     /label=-10 box
     promoter        1..59
                     /label=BBa_K390026
     misc_feature    66..68
                     /label=Start
     CDS             66..785
                     /label=GFP Coding Region
     CDS             66..785
                     /label=BBa_K208000
     stem_loop       805..848
                     /label=stem_loop
     misc_feature    794..873
                     /label=BBa_B0010
     terminator      794..873
                     /label=BBa_B0010
     stem_loop       889..908
                     /label=T7 TE
     misc_feature    915..915
                     /label=stop
     polyA_site      909..922
                     /label=polya
     misc_feature    882..922
                     /label=BBa_B0012
     terminator      882..922
                     /label=BBa_B0012
     misc_feature    66..922
                     /label=BBa_K208014
ORIGIN
        1 tgatgtaggc agagtccaac catcggtaaa gttgattaga gtcgcccaaa cctaaaacct
       61 actagatggc tagcaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat
      121 tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcta
      181 catacggaaa gcttaccctt aaatttattt gcactactgg aaaactacct gttccatggc
      241 caacacttgt cactactttc tcttatggtg ttcaatgctt ttcccgttat ccggatcata
      301 tgaaacggca tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaacgcacta
      361 tatctttcaa agatgacggg aactacaaga cgcgtgctga agtcaagttt gaaggtgata
      421 cccttgttaa tcgtatcgag ttaaaaggta ttgattttaa agaagatgga aacattctcg
      481 gacacaaact cgagtacaac tataactcac acaatgtata catcacggca gacaaacaaa
      541 agaatggaat caaagctaac ttcaaaattc gccacaacat tgaagatgga tccgttcaac
      601 tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca
      661 accattacct gtcgacacaa tctgcccttt cgaaagatcc caacgaaaag cgtgaccaca
      721 tggtccttct tgagtttgta actgctgctg ggattacaca tggcatggat gagctctaca
      781 aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
      841 cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg
      901 ggtgggcctt tctgcgttta ta
//