BBa_K208000
1
BBa_K208000
GFP
2009-10-07T11:00:00Z
2015-05-08T01:11:24Z
plasmid
GFP
false
false
_310_
0
2425
303
It's complicated
true
Mutate PstI sites
false
Elisabeth Linton
annotation2062216
1
Start
range2062216
1
1
3
annotation2062217
1
GFP Coding Region
range2062217
1
1
720
BBa_K390004
1
BBa_K390004
art+ secretion tag for Synechocystis sp. PCC 6803
2010-07-31T11:00:00Z
2015-05-08T01:12:19Z
This part is based on a sequence that was artificially designed in Sergeyenko and Los, 2003. It was created by annealing two oligionucleotides together.
In ''Synechocystis'' sp. PCC 6803, the system of secretion is based on the charge of the N-terminus leader sequence. Positive charges on the N-terminus leader sequence contribute to secretion in a non-sequence-dependent manner (Sergeyenko TV and Los DA. 2003. Cyanobacterial leader peptides for protein secretion. FEMS Microbiology Letters 218: 351-357).
This part is an artificially designed secretion tag from the above paper. It is composed of positively charged amino acids, and was shown to successfully function as a secretion tag in ''Synechocystis'' in the above paper.
false
false
_518_
0
6788
9
It's complicated
false
none
false
Cody Tramp
annotation2076216
1
secretion tag - Synechocystis sp. PCC 6803
range2076216
1
1
64
annotation2076215
1
Start Codon
range2076215
1
1
3
BBa_K390062
1
BBa_K390062
GFP with art+ secretion tag for Synechocystis sp. PCC 6803
2010-07-31T11:00:00Z
2015-05-08T01:12:20Z
composite part
Cycle 3 mutant GFP with artifically designed secretion tag (Sergeyenko TV and Los DA. 2003. Cyanobacterial leader peptides for protein secretion. FEMS Microbiology Letters 218: 351-357) for use in Synechocystis sp. PCC 6803.
This should be a highly secreted construct (verification pending).
false
false
_518_
0
6788
9
Not in stock
false
none
false
Cody Tramp
component2111518
1
BBa_K390004
component2111526
1
BBa_K208014
annotation2111518
1
BBa_K390004
range2111518
1
1
64
annotation2111526
1
BBa_K208014
range2111526
1
71
927
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1686
1
T7 TE
range1686
1
8
27
annotation1690
1
polya
range1690
1
28
41
BBa_K208014
1
BBa_K208014
GFP (Silver Fusion)/Double Terminator
2009-10-19T11:00:00Z
2015-05-08T01:11:24Z
Composite of GFP with BioBrick Terminator
GFP with Terminator
false
true
_310_
0
3473
9
It's complicated
false
Silver Fusion
false
USU iGEM 2009
component2056813
1
BBa_B0012
component2056811
1
BBa_B0010
component2056810
1
BBa_K208000
annotation2056813
1
BBa_B0012
range2056813
1
817
857
annotation2056810
1
BBa_K208000
range2056810
1
1
720
annotation2056811
1
BBa_B0010
range2056811
1
729
808
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K208014_sequence
1
atggctagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaagcttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttctcttatggtgttcaatgcttttcccgttatccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactcgagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggatccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K390004_sequence
1
atggatagaaagcacgtgctccggaaaaaagaagactctgcgcggaaaaaacaaaaaggtggta
BBa_K390062_sequence
1
atggatagaaagcacgtgctccggaaaaaagaagactctgcgcggaaaaaacaaaaaggtggtatactagatggctagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaagcttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttctcttatggtgttcaatgcttttcccgttatccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactcgagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggatccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K208000_sequence
1
atggctagcaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgctacatacggaaagcttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttctcttatggtgttcaatgcttttcccgttatccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactcgagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggatccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaataa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z