LOCUS       BBa_K208014              857 bp    DNA     linear       26-Nov-2024
DEFINITION  GFP (Silver Fusion)/Double Terminator
ACCESSION   BBa_K208014
VERSION     BBa_K208014.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=Start
     CDS             1..720
                     /label=GFP Coding Region
     CDS             1..720
                     /label=BBa_K208000
     stem_loop       740..783
                     /label=stem_loop
     misc_feature    729..808
                     /label=BBa_B0010
     terminator      729..808
                     /label=BBa_B0010
     stem_loop       824..843
                     /label=T7 TE
     misc_feature    850..850
                     /label=stop
     polyA_site      844..857
                     /label=polya
     misc_feature    817..857
                     /label=BBa_B0012
     terminator      817..857
                     /label=BBa_B0012
ORIGIN
        1 atggctagca aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat
       61 ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgctacatac
      121 ggaaagctta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca
      181 cttgtcacta ctttctctta tggtgttcaa tgcttttccc gttatccgga tcatatgaaa
      241 cggcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaacg cactatatct
      301 ttcaaagatg acgggaacta caagacgcgt gctgaagtca agtttgaagg tgataccctt
      361 gttaatcgta tcgagttaaa aggtattgat tttaaagaag atggaaacat tctcggacac
      421 aaactcgagt acaactataa ctcacacaat gtatacatca cggcagacaa acaaaagaat
      481 ggaatcaaag ctaacttcaa aattcgccac aacattgaag atggatccgt tcaactagca
      541 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat
      601 tacctgtcga cacaatctgc cctttcgaaa gatcccaacg aaaagcgtga ccacatggtc
      661 cttcttgagt ttgtaactgc tgctgggatt acacatggca tggatgagct ctacaaataa
      721 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
      781 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
      841 gcctttctgc gtttata
//
LOCUS       BBa_K390064              857 bp    DNA     linear       26-Nov-2024
DEFINITION  eGFP + Terminator
ACCESSION   BBa_K390064
VERSION     BBa_K390064.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=Start
     CDS             1..720
                     /label=GFP Coding Region
     CDS             1..720
                     /label=BBa_K208000
     stem_loop       740..783
                     /label=stem_loop
     misc_feature    729..808
                     /label=BBa_B0010
     terminator      729..808
                     /label=BBa_B0010
     stem_loop       824..843
                     /label=T7 TE
     misc_feature    850..850
                     /label=stop
     polyA_site      844..857
                     /label=polya
     misc_feature    817..857
                     /label=BBa_B0012
     terminator      817..857
                     /label=BBa_B0012
ORIGIN
        1 atggctagca aaggagaaga acttttcact ggagttgtcc caattcttgt tgaattagat
       61 ggtgatgtta atgggcacaa attttctgtc agtggagagg gtgaaggtga tgctacatac
      121 ggaaagctta cccttaaatt tatttgcact actggaaaac tacctgttcc atggccaaca
      181 cttgtcacta ctttctctta tggtgttcaa tgcttttccc gttatccgga tcatatgaaa
      241 cggcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaacg cactatatct
      301 ttcaaagatg acgggaacta caagacgcgt gctgaagtca agtttgaagg tgataccctt
      361 gttaatcgta tcgagttaaa aggtattgat tttaaagaag atggaaacat tctcggacac
      421 aaactcgagt acaactataa ctcacacaat gtatacatca cggcagacaa acaaaagaat
      481 ggaatcaaag ctaacttcaa aattcgccac aacattgaag atggatccgt tcaactagca
      541 gaccattatc aacaaaatac tccaattggc gatggccctg tccttttacc agacaaccat
      601 tacctgtcga cacaatctgc cctttcgaaa gatcccaacg aaaagcgtga ccacatggtc
      661 cttcttgagt ttgtaactgc tgctgggatt acacatggca tggatgagct ctacaaataa
      721 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
      781 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
      841 gcctttctgc gtttata
//