LOCUS       BBa_K390501             1136 bp    DNA     linear       04-May-2024
DEFINITION  LacPromoter/RBS/PhaP/HlyA/Terminator
ACCESSION   BBa_K390501
VERSION     BBa_K390501.1  
FEATURES             Location/Qualifiers
     RBS             209..220
                     /label=BBa_B0034
     promoter        1..200
                     /label=BBa_R0010
     promoter        1..220
                     /label=BBa_K208010
     misc_feature    227..229
                     /label=Start
     CDS             227..805
                     /label=Phasin
     CDS             227..805
                     /label=BBa_K208001
     misc_feature    814..999
                     /label=HlyA
     misc_feature    814..816
                     /label=Start
     misc_feature    814..999
                     /label=BBa_K208006
     stem_loop       1019..1062
                     /label=stem_loop
     misc_feature    1008..1087
                     /label=BBa_B0010
     terminator      1008..1087
                     /label=BBa_B0010
     stem_loop       1103..1122
                     /label=T7 TE
     misc_feature    1129..1129
                     /label=stop
     polyA_site      1123..1136
                     /label=polya
     misc_feature    1096..1136
                     /label=BBa_B0012
     terminator      1096..1136
                     /label=BBa_B0012
     misc_feature    227..1136
                     /label=BBa_K208029
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga tcctcacccc
      241 ggaacaagtt gcagcagcgc aaaaggccaa cctcgaaacg ctgttcggcc tgaccaccaa
      301 ggcgtttgaa ggcgtcgaaa agctcgtcga gctgaacctt caggtcgtca agacttcgtt
      361 cgcagaaggc gttgacaacg ccaagaaggc gctgtcggcc aaggacgcac aggaactgct
      421 ggccatccag gccgcagccg tgcagccggt tgccgaaaag accctggcct acacccgcca
      481 cctgtatgaa atcgcttcgg aaacccagag cgagttcacc aaggtagccg aggctcaact
      541 ggccgaaggc tcgaagaacg tgcaagcgct ggtcgagaac ctcgccaaga acgccccggc
      601 cggttcggaa tcgaccgtgg ccatcgtgaa gtcggcgatc tccgctgcca acaacgccta
      661 cgagtcggtg cagaaggcga ccaagcaagc ggtcgaaatc gctgaaacca acttccaggc
      721 tgcggctacg gctgccacca aggctgccca gcaagccagc gccacggccc gtacggccac
      781 ggcaaagaag acgacggctg cctgatacta gagttagcct atggaagtca gggtgatctt
      841 aatccattaa ttaatgaaat cagcaaaatc atttcagcag caggtagctt cgatgttaaa
      901 gaggaaagaa ccgcagcttc tttattgcag ttgtccggta atgccagtga tttttcatat
      961 ggacggaact caataaccct gaccacatca gcataataat actagagcca ggcatcaaat
     1021 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     1081 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//