LOCUS       BBa_K390502             1277 bp    DNA     linear       27-Dec-2024
DEFINITION  LacPromoter/RBS/GFP/HlyA/Terminator
ACCESSION   BBa_K390502
VERSION     BBa_K390502.1  
FEATURES             Location/Qualifiers
     RBS             209..220
                     /label=BBa_B0034
     promoter        1..200
                     /label=BBa_R0010
     promoter        1..220
                     /label=BBa_K208010
     misc_feature    227..229
                     /label=Start
     CDS             227..946
                     /label=GFP Coding Region
     CDS             227..946
                     /label=BBa_K208000
     misc_feature    955..1140
                     /label=HlyA
     misc_feature    955..957
                     /label=Start
     misc_feature    955..1140
                     /label=BBa_K208006
     stem_loop       1160..1203
                     /label=stem_loop
     misc_feature    1149..1228
                     /label=BBa_B0010
     terminator      1149..1228
                     /label=BBa_B0010
     stem_loop       1244..1263
                     /label=T7 TE
     misc_feature    1270..1270
                     /label=stop
     polyA_site      1264..1277
                     /label=polya
     misc_feature    1237..1277
                     /label=BBa_B0012
     terminator      1237..1277
                     /label=BBa_B0012
     misc_feature    227..1277
                     /label=BBa_K208030
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgg ctagcaaagg
      241 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg
      301 gcacaaattt tctgtcagtg gagagggtga aggtgatgct acatacggaa agcttaccct
      361 taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt
      421 ctcttatggt gttcaatgct tttcccgtta tccggatcat atgaaacggc atgacttttt
      481 caagagtgcc atgcccgaag gttatgtaca ggaacgcact atatctttca aagatgacgg
      541 gaactacaag acgcgtgctg aagtcaagtt tgaaggtgat acccttgtta atcgtatcga
      601 gttaaaaggt attgatttta aagaagatgg aaacattctc ggacacaaac tcgagtacaa
      661 ctataactca cacaatgtat acatcacggc agacaaacaa aagaatggaa tcaaagctaa
      721 cttcaaaatt cgccacaaca ttgaagatgg atccgttcaa ctagcagacc attatcaaca
      781 aaatactcca attggcgatg gccctgtcct tttaccagac aaccattacc tgtcgacaca
      841 atctgccctt tcgaaagatc ccaacgaaaa gcgtgaccac atggtccttc ttgagtttgt
      901 aactgctgct gggattacac atggcatgga tgagctctac aaataatact agagttagcc
      961 tatggaagtc agggtgatct taatccatta attaatgaaa tcagcaaaat catttcagca
     1021 gcaggtagct tcgatgttaa agaggaaaga accgcagctt ctttattgca gttgtccggt
     1081 aatgccagtg atttttcata tggacggaac tcaataaccc tgaccacatc agcataataa
     1141 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     1201 tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg
     1261 gcctttctgc gtttata
//