LOCUS BBa_K390777 3560 bp DNA linear 05-Nov-2024 DEFINITION intermediate ACCESSION BBa_K390777 VERSION BBa_K390777.1 FEATURES Location/Qualifiers misc_feature 3..19 /label=lac O1 promoter 20..25 /label=-35 misc_feature 26..42 /label=lac O1 promoter 43..48 /label=-10 misc_feature 1..54 /label=BBa_R0011 promoter 1..54 /label=BBa_R0011 misc_feature 68..71 /label=conserved RBS 64..75 /label=BBa_B0034 misc_feature 82..83 /label=prefix misc_feature 255..255 /label=T misc_feature 573..573 /label=A CDS 82..831 /label=luxR misc_feature 82..837 /label=BBa_C0062 misc_feature 838..862 /label=Help:Barcodes CDS 82..837 /label=BBa_C0062 stem_loop 882..925 /label=stem_loop misc_feature 871..950 /label=BBa_B0010 terminator 871..950 /label=BBa_B0010 stem_loop 966..985 /label=T7 TE misc_feature 992..992 /label=stop polyA_site 986..999 /label=polya misc_feature 959..999 /label=BBa_B0012 terminator 959..999 /label=BBa_B0012 misc_feature 1008..1027 /label=LuxR/HSL promoter 1027..1032 /label=-35 promoter 1049..1054 /label=-10 misc_feature 1060..1060 /label=start misc_feature 1008..1062 /label=BBa_R0062 promoter 1008..1062 /label=BBa_R0062 misc_feature 1..1062 /label=BBa_F2622 misc_feature 1075..1078 /label=conserved RBS 1071..1082 /label=BBa_B0034 misc_feature 1089..1091 /label=Start CDS 1089..1808 /label=GFP Coding Region CDS 1089..1808 /label=BBa_K208000 stem_loop 1828..1871 /label=stem_loop misc_feature 1817..1896 /label=BBa_B0010 terminator 1817..1896 /label=BBa_B0010 stem_loop 1912..1931 /label=T7 TE misc_feature 1938..1938 /label=stop polyA_site 1932..1945 /label=polya misc_feature 1905..1945 /label=BBa_B0012 terminator 1905..1945 /label=BBa_B0012 RBS 1954..1966 /label=RBS-3\Weak RBS 1960..1963 /label=RBS misc_feature 1954..1966 /label=BBa_B0032 RBS 1954..1966 /label=BBa_B0032 misc_feature 1973..1974 /label=prefix misc_feature 2146..2146 /label=T misc_feature 2464..2464 /label=A CDS 1973..2722 /label=luxR misc_feature 1973..2728 /label=BBa_C0062 misc_feature 2729..2753 /label=Help:Barcodes CDS 1973..2728 /label=BBa_C0062 misc_feature 1954..2753 /label=BBa_K116616 RBS 2762..2774 /label=RBS-3\Weak RBS 2768..2771 /label=RBS misc_feature 2762..2774 /label=BBa_B0032 RBS 2762..2774 /label=BBa_B0032 misc_feature 3360..3391 /label=LVA CDS 2781..3359 /label=luxI misc_feature 2781..3398 /label=BBa_C0061 misc_feature 3399..3423 /label=Help:Barcodes CDS 2781..3398 /label=BBa_C0061 stem_loop 3443..3486 /label=stem_loop misc_feature 3432..3511 /label=BBa_B0010 terminator 3432..3511 /label=BBa_B0010 stem_loop 3527..3546 /label=T7 TE misc_feature 3553..3553 /label=stop polyA_site 3547..3560 /label=polya misc_feature 3520..3560 /label=BBa_B0012 terminator 3520..3560 /label=BBa_B0012 terminator 3432..3560 /label=BBa_B0015 misc_feature 2781..3560 /label=BBa_K116615 misc_feature 2762..3560 /label=BBa_K116625 misc_feature 1954..3560 /label=BBa_K116634 ORIGIN 1 aattgtgagc ggataacaat tgacattgtg agcggataac aagatactga gcacatacta 61 gagaaagagg agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt 121 aataaaatta aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact 181 aaaatggtac attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa 241 tctgatattt caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct 301 aatttaataa aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat 361 tggaatatat ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg 421 aaaacatcag gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc 481 ggaatgctta gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat 541 gcgtgtatga acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat 601 atagcaaata ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg 661 gcatgcgaag gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact 721 gtcactttcc atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt 781 atttctaaag caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac 841 tgatagtgct agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt 901 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 961 acactggctc accttcgggt gggcctttct gcgtttatat actagagacc tgtaggatcg 1021 tacaggttta cgcaagaaaa tggtttgtta tagtcgaata aatactagag aaagaggaga 1081 aatactagat ggctagcaaa ggagaagaac ttttcactgg agttgtccca attcttgttg 1141 aattagatgg tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg 1201 ctacatacgg aaagcttacc cttaaattta tttgcactac tggaaaacta cctgttccat 1261 ggccaacact tgtcactact ttctcttatg gtgttcaatg cttttcccgt tatccggatc 1321 atatgaaacg gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaacgca 1381 ctatatcttt caaagatgac gggaactaca agacgcgtgc tgaagtcaag tttgaaggtg 1441 atacccttgt taatcgtatc gagttaaaag gtattgattt taaagaagat ggaaacattc 1501 tcggacacaa actcgagtac aactataact cacacaatgt atacatcacg gcagacaaac 1561 aaaagaatgg aatcaaagct aacttcaaaa ttcgccacaa cattgaagat ggatccgttc 1621 aactagcaga ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag 1681 acaaccatta cctgtcgaca caatctgccc tttcgaaaga tcccaacgaa aagcgtgacc 1741 acatggtcct tcttgagttt gtaactgctg ctgggattac acatggcatg gatgagctct 1801 acaaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc 1861 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct 1921 tcgggtgggc ctttctgcgt ttatatacta gagtcacaca ggaaagtact agatgaaaaa 1981 cataaatgcc gacgacacat acagaataat taataaaatt aaagcttgta gaagcaataa 2041 tgatattaat caatgcttat ctgatatgac taaaatggta cattgtgaat attatttact 2101 cgcgatcatt tatcctcatt ctatggttaa atctgatatt tcaatcctag ataattaccc 2161 taaaaaatgg aggcaatatt atgatgacgc taatttaata aaatatgatc ctatagtaga 2221 ttattctaac tccaatcatt caccaattaa ttggaatata tttgaaaaca atgctgtaaa 2281 taaaaaatct ccaaatgtaa ttaaagaagc gaaaacatca ggtcttatca ctgggtttag 2341 tttccctatt catacggcta acaatggctt cggaatgctt agttttgcac attcagaaaa 2401 agacaactat atagatagtt tatttttaca tgcgtgtatg aacataccat taattgttcc 2461 ttctctagtt gataattatc gaaaaataaa tatagcaaat aataaatcaa acaacgattt 2521 aaccaaaaga gaaaaagaat gtttagcgtg ggcatgcgaa ggaaaaagct cttgggatat 2581 ttcaaaaata ttaggttgca gtgagcgtac tgtcactttc catttaacca atgcgcaaat 2641 gaaactcaat acaacaaacc gctgccaaag tatttctaaa gcaattttaa caggagcaat 2701 tgattgccca tactttaaaa attaataaca ctgatagtgc tagtgtagat cactactaga 2761 gtcacacagg aaagtactag atgactataa tgataaaaaa atcggatttt ttggcaattc 2821 catcggagga gtataaaggt attctaagtc ttcgttatca agtgtttaag caaagacttg 2881 agtgggactt agttgtagaa aataaccttg aatcagatga gtatgataac tcaaatgcag 2941 aatatattta tgcttgtgat gatactgaaa atgtaagtgg atgctggcgt ttattaccta 3001 caacaggtga ttatatgctg aaaagtgttt ttcctgaatt gcttggtcaa cagagtgctc 3061 ccaaagatcc taatatagtc gaattaagtc gttttgctgt aggtaaaaat agctcaaaga 3121 taaataactc tgctagtgaa attacaatga aactatttga agctatatat aaacacgctg 3181 ttagtcaagg tattacagaa tatgtaacag taacatcaac agcaatagag cgatttttaa 3241 agcgtattaa agttccttgt catcgtattg gagacaaaga aattcatgta ttaggtgata 3301 ctaaatcggt tgtattgtct atgcctatta atgaacagtt taaaaaagca gtcttaaatg 3361 ctgcaaacga cgaaaactac gctttagtag cttaataact ctgatagtgc tagtgtagat 3421 ctctactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg 3481 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg 3541 tgggcctttc tgcgtttata //