BBa_K391004 1 BBa_K391004 Forward primer to PCR S. aureus plasmid replicon 2010-10-24T11:00:00Z 2015-05-08T01:12:20Z From Charpentier, et al.. Novel Cassette-Based Shuttle Vector System for Gram-Positive Bacteria. Appl Environ Microbiol. 2004 October; 70(10): 6076???6085. Refer to pT181 gene bank information. S. aureus plasmid replicon. Can allow a BioBrick plasmid to replicate and segregate in S. aureus. Derived from pT181. Can use either plasmids pT181 or plasmids derived from pT181 (e.g. pcN33). Add EcoRI and SpeI sites before using. Expect a band ~2kbp after PCR. false false _531_ 0 6505 9 It's complicated false Note a base pair difference between the forward primer given in the paper and the pT181 gene bank information. UBC iGEM used pT181 sequence information instead. false Phillip Chu BBa_K391004_sequence 1 tttgcggaaagagttaataagttaacag igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z