BBa_K416003 1 BBa_K416003 Yeast Secretion Tag 2010-06-22T11:00:00Z 2015-05-08T01:12:27Z This part was sent to use from Dr. Sheldon Park of the University of Buffalo. This secretion tag when linked to the N-terminal of the protein directs extracellular secretion of the protein. After targeting through the ER to the golgi, this "pre-pro" tag is cleaved by the KEX-2 protease resulting in secretion of protein without the aforementioned tag. To use this tag, attached it directly upstream of your proteins CDS. false false _527_ 0 7430 9 It's complicated true There was an illegal Xbal1 site at bp 101 and we removed it through altering the primers necessary for biobricking the sequence. false John Phair BBa_K392030 1 BBa_K392030 yeast secretion tag + C. fermi endoglucanase 2010-10-24T11:00:00Z 2015-05-08T01:12:20Z Silver assembly of K416003 with K392006 C. fermi endoglucanase CenA fused with engineered yeast secretion tag for extracellular expression. false false _528_ 0 6220 9 It's complicated false Assembled from Silver standard-compatible parts. Sequence shown on part's main page may not be accurate since it assumes Standard assembly. Please check sequencing results from Get This Part page instead. false Teoh Shao Thing, Rie Takino, Kosuke Torigata component2098175 1 BBa_K392006 component2098174 1 BBa_K416003 annotation2098175 1 BBa_K392006 range2098175 1 123 1466 annotation2098174 1 BBa_K416003 range2098174 1 1 114 BBa_K392006 1 BBa_K392006 C. firmi CenA (Silver standard compatible) 2010-10-23T11:00:00Z 2015-05-08T01:12:20Z Registry part <partinfo>BBa_K118023</partinfo>. Endoglucanase from C. firmi re-cloned into Silver standard-compatible part. false false _528_ 0 6220 9 It's complicated true Created from <partinfo>BBa_K118023</partinfo> by PCR. false Tadashi Nakamura, Shuhei Yasumoto, Takahiro Saka, Saya Kakuda BBa_K392006_sequence 1 tccacccgcagaaccgccgcagcgctgctggcggccgcggccgtcgccgtcggcggtctgaccgccctcaccaccaccgccgcgcaggcggctcccggctgccgcgtcgactacgccgtcaccaaccagtggcccggcggcttcggcgccaacgtcacgatcaccaacctcggcgaccccgtctcgtcgtggaagctcgactggacctacaccgcaggccagcggatccagcagctgtggaacggcaccgcgtcgaccaacggcggccaggtctccgtcaccagcctgccctggaacggcagcatcccgaccggcggcacggcgtcgttcgggttcaacggctcgtgggccgggtccaacccgacgccggcgtcgttctcgctcaacggcaccacctgcacgggcaccgtgccgacgaccagccccacgccgaccccgacgccgacgacccccacgccgacgccgaccccgacccccacccccacgccgacggtcacgccgcagccgaccagcggcttctacgtcgacccgacgacgcagggctaccgcgcgtggcaggccgcgtccggcacggacaaggcgctgctcgagaagatcgcgctcaccccgcaggcgtactgggtcggcaactgggccgacgcgtcgcacgcgcaggccgaggtcgccgactacaccggccgcgccgtcgcggccgggaagacgccgatgctcgtcgtctacgcgatcccgggccgcgactgcggctcgcactccggcggtggtgtgtccgagtccgagtacgcgcgctgggtcgacaccgtcgcgcagggcatcaagggcaacccgatcgtgatcctcgagcccgacgcgctcgcgcagctcggcgactgctccggccagggtgaccgcgtcggcttcctcaagtacgccgccaagtcgctcaccctcaagggcgcgcgcgtctacatcgacgcgggccacgcgaagtggctgtcggtcgacacgccggtgaaccgcctcaaccaggtcggcttcgagtacgcggtgggcttcgcgctcaacacgtcgaactaccagacgacggcggacagcaaggcgtacggccagcagatctcgcagcggctgggcggcaagaagttcgtcatcgacacctcgcgcaacggcaacggctcgaacggcgagtggtgcaacccgcgcggccgcgcgctcggcgaacgcccggtcgcggtgaacgacggctccggcctggacgcgctcctgtgggtcaagctgcccggcgagtccgacggcgcgtgcaacggcggcccggccgccggccagtggtggcaggagatcgccctggagatggcgcgcaacgccaggtgg BBa_K416003_sequence 1 atgaaagttttgattgttttgttggctattttcgctgctttgccattggctttggctcaaccagttatttctactactgttggttctgctgctgaaggttcactagataaaaga BBa_K392030_sequence 1 atgaaagttttgattgttttgttggctattttcgctgctttgccattggctttggctcaaccagttatttctactactgttggttctgctgctgaaggttcactagataaaagatactagagtccacccgcagaaccgccgcagcgctgctggcggccgcggccgtcgccgtcggcggtctgaccgccctcaccaccaccgccgcgcaggcggctcccggctgccgcgtcgactacgccgtcaccaaccagtggcccggcggcttcggcgccaacgtcacgatcaccaacctcggcgaccccgtctcgtcgtggaagctcgactggacctacaccgcaggccagcggatccagcagctgtggaacggcaccgcgtcgaccaacggcggccaggtctccgtcaccagcctgccctggaacggcagcatcccgaccggcggcacggcgtcgttcgggttcaacggctcgtgggccgggtccaacccgacgccggcgtcgttctcgctcaacggcaccacctgcacgggcaccgtgccgacgaccagccccacgccgaccccgacgccgacgacccccacgccgacgccgaccccgacccccacccccacgccgacggtcacgccgcagccgaccagcggcttctacgtcgacccgacgacgcagggctaccgcgcgtggcaggccgcgtccggcacggacaaggcgctgctcgagaagatcgcgctcaccccgcaggcgtactgggtcggcaactgggccgacgcgtcgcacgcgcaggccgaggtcgccgactacaccggccgcgccgtcgcggccgggaagacgccgatgctcgtcgtctacgcgatcccgggccgcgactgcggctcgcactccggcggtggtgtgtccgagtccgagtacgcgcgctgggtcgacaccgtcgcgcagggcatcaagggcaacccgatcgtgatcctcgagcccgacgcgctcgcgcagctcggcgactgctccggccagggtgaccgcgtcggcttcctcaagtacgccgccaagtcgctcaccctcaagggcgcgcgcgtctacatcgacgcgggccacgcgaagtggctgtcggtcgacacgccggtgaaccgcctcaaccaggtcggcttcgagtacgcggtgggcttcgcgctcaacacgtcgaactaccagacgacggcggacagcaaggcgtacggccagcagatctcgcagcggctgggcggcaagaagttcgtcatcgacacctcgcgcaacggcaacggctcgaacggcgagtggtgcaacccgcgcggccgcgcgctcggcgaacgcccggtcgcggtgaacgacggctccggcctggacgcgctcctgtgggtcaagctgcccggcgagtccgacggcgcgtgcaacggcggcccggccgccggccagtggtggcaggagatcgccctggagatggcgcgcaacgccaggtgg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z