BBa_K416003 1 BBa_K416003 Yeast Secretion Tag 2010-06-22T11:00:00Z 2015-05-08T01:12:27Z This part was sent to use from Dr. Sheldon Park of the University of Buffalo. This secretion tag when linked to the N-terminal of the protein directs extracellular secretion of the protein. After targeting through the ER to the golgi, this "pre-pro" tag is cleaved by the KEX-2 protease resulting in secretion of protein without the aforementioned tag. To use this tag, attached it directly upstream of your proteins CDS. false false _527_ 0 7430 9 It's complicated true There was an illegal Xbal1 site at bp 101 and we removed it through altering the primers necessary for biobricking the sequence. false John Phair BBa_J63003 1 Kozak & st designed yeast Kozak sequence 2006-10-10T11:00:00Z 2015-08-31T01:56:26Z consensus Kozak and start built from oligonucleotides Released HQ 2013 consensus Kozak sequence and start codon false false _97_ 0 545 97 In stock false designed such that fusion using fusion bricks assembly method leads to in frame translation true Caroline Ajo-Franklin annotation1902852 1 start codon range1902852 1 13 15 BBa_K392003 1 BBa_K392003 yeast ADH1 terminator 2010-10-09T11:00:00Z 2015-05-08T01:12:20Z Registry parts BBa_K105027 (promoter) and BBa_J63003 (Kozak). Engineered 'cyc100 promoter' of <i>Saccharomyces cerevisiae</i> with Kozak sequence attached downstream. false false _528_ 0 6220 9 It's complicated true Assembled by 3A method. false Tadashi Nakamura, Shuhei Yasumoto, Takahiro Saka, Saya Kakuda BBa_K392014 1 BBa_K392014 C. josui CM10 2010-10-24T11:00:00Z 2015-05-08T01:12:20Z Xyn10 plasmid received from Shuichi Karita of Faculty of Bioresources, Mie University (Japan). Cellulose-binding motif from C. josui Xyn10A gene, cloned into Silver standard-compatible part. false false _528_ 0 6220 9 It's complicated true Cloned from original plasmid by PCR. false Tadashi Nakamura, Shuhei Yasumoto, Takahiro Saka, Saya Kakuda BBa_K392006 1 BBa_K392006 C. firmi CenA (Silver standard compatible) 2010-10-23T11:00:00Z 2015-05-08T01:12:20Z Registry part <partinfo>BBa_K118023</partinfo>. Endoglucanase from C. firmi re-cloned into Silver standard-compatible part. false false _528_ 0 6220 9 It's complicated true Created from <partinfo>BBa_K118023</partinfo> by PCR. false Tadashi Nakamura, Shuhei Yasumoto, Takahiro Saka, Saya Kakuda BBa_K392036 1 BBa_K392036 endoglucanase/CBM secretion testing construct for yeast 2010-10-24T11:00:00Z 2015-05-08T01:12:21Z Silver assembly of J63005, K416003, K392006, K392014, K392003. Yeast expression device containing C. fermi endoglucanase CenA fused with cellulose-binding motif CM10; engineered secretion tag attached upstream to enable secretion of fusion protein. false false _528_ 0 6220 9 It's complicated false Assembled from Silver standard-compatible parts. Sequence shown on part's main page may not be accurate since it assumes Standard assembly. Please check sequencing results from Get This Part page instead. false Teoh Shao Thing, Rie Takino, Kosuke Torigata component2100437 1 BBa_K416003 component2100438 1 BBa_K392006 component2100436 1 BBa_J63005 component2100439 1 BBa_K392014 component2100444 1 BBa_K392003 component2100442 1 BBa_J63003 component2100440 1 BBa_K105027 annotation2100442 1 BBa_J63003 range2100442 1 4128 4145 annotation2100437 1 BBa_K416003 range2100437 1 1452 1565 annotation2100439 1 BBa_K392014 range2100439 1 2926 4008 annotation2100440 1 BBa_K105027 range2100440 1 4017 4119 annotation2100444 1 BBa_K392003 range2100444 1 4017 4145 annotation2100436 1 BBa_J63005 range2100436 1 1 1445 annotation2100438 1 BBa_K392006 range2100438 1 1574 2917 BBa_J63005 1 P0310 yeast ADH1 promoter 2006-10-10T11:00:00Z 2015-08-31T01:56:26Z genomic DNA of ADH1 from S. cerevisiae ADH1 promoter from S. cerevisiae false true _97_ 0 545 97 It's complicated false no changes to genomic sequence true Caroline Ajo-Franklin annotation1902745 1 TATA Box range1902745 1 1348 1354 annotation1902744 1 Constitutive promoter range1902744 1 1 1445 BBa_K105027 1 BBa_K105027 cyc100 minimal promoter 2008-10-25T11:00:00Z 2015-05-08T01:08:52Z Amplification from the plasmid pAH3. This is the core of the CYC1 promoter of ''S. cerevisiae''. This promoter element allows basal transcription. The transcription signal can be modulated by adding operator sites upstream of this BioBrick. In that way activators or repressors can act on the transcription machinery. <br> <br< false false _253_ 0 3313 9 It's complicated true This BioBrick contains the TATA-box and most of the 5'UTR (-139 to -36, relative to the translational start)of the natural CYC1 promoter. A Kozak sequence is not included and has thus to be added! true Michael Wild annotation1989774 1 TATA box range1989774 1 17 23 BBa_K392006_sequence 1 tccacccgcagaaccgccgcagcgctgctggcggccgcggccgtcgccgtcggcggtctgaccgccctcaccaccaccgccgcgcaggcggctcccggctgccgcgtcgactacgccgtcaccaaccagtggcccggcggcttcggcgccaacgtcacgatcaccaacctcggcgaccccgtctcgtcgtggaagctcgactggacctacaccgcaggccagcggatccagcagctgtggaacggcaccgcgtcgaccaacggcggccaggtctccgtcaccagcctgccctggaacggcagcatcccgaccggcggcacggcgtcgttcgggttcaacggctcgtgggccgggtccaacccgacgccggcgtcgttctcgctcaacggcaccacctgcacgggcaccgtgccgacgaccagccccacgccgaccccgacgccgacgacccccacgccgacgccgaccccgacccccacccccacgccgacggtcacgccgcagccgaccagcggcttctacgtcgacccgacgacgcagggctaccgcgcgtggcaggccgcgtccggcacggacaaggcgctgctcgagaagatcgcgctcaccccgcaggcgtactgggtcggcaactgggccgacgcgtcgcacgcgcaggccgaggtcgccgactacaccggccgcgccgtcgcggccgggaagacgccgatgctcgtcgtctacgcgatcccgggccgcgactgcggctcgcactccggcggtggtgtgtccgagtccgagtacgcgcgctgggtcgacaccgtcgcgcagggcatcaagggcaacccgatcgtgatcctcgagcccgacgcgctcgcgcagctcggcgactgctccggccagggtgaccgcgtcggcttcctcaagtacgccgccaagtcgctcaccctcaagggcgcgcgcgtctacatcgacgcgggccacgcgaagtggctgtcggtcgacacgccggtgaaccgcctcaaccaggtcggcttcgagtacgcggtgggcttcgcgctcaacacgtcgaactaccagacgacggcggacagcaaggcgtacggccagcagatctcgcagcggctgggcggcaagaagttcgtcatcgacacctcgcgcaacggcaacggctcgaacggcgagtggtgcaacccgcgcggccgcgcgctcggcgaacgcccggtcgcggtgaacgacggctccggcctggacgcgctcctgtgggtcaagctgcccggcgagtccgacggcgcgtgcaacggcggcccggccgccggccagtggtggcaggagatcgccctggagatggcgcgcaacgccaggtgg BBa_K392014_sequence 1 attgacgatattagctttgaacaaacgggttcaggaccaatcaaaatccaagatgatttaaaatccattaaagatgtatataaaaaggatttccttattggaaacgcaatagcagcagaggatatggaaggggtcagacttgacctgttaaagaagcactttaatgtaatgacagctggtaatgctatgaagccagatgcattacaaccttcaaagggtaactttacatttgaagatgcagataagcttgttgataaggttctggcagagggattcaagatgcatggacatactcttgtttggcatcagcagtcacctgcttggttaaatacaaaagttgatgcaagcggaaatgctatacctttgtcacgtgaggaagctcttacaaatctaagaacccatattaagacagttgtagagcactacggtaacaaggtaatatcatgggacgtagtaaatgaaggtatgaatgataatcctactaatcctgctgactggaaagcatcattacgtcaaagcccatggtatcaggcaattggtcctgattacatagaagaggcgtttttagcagcaagagaggttctggatgctcaccctgattgggatgtgaagctatattataatgattataacgatgataatcaaagcaaatctaaagctatttactacatggtaaaagagttaaatgaaaaatatgcaaaaactcaccctggtaagcttcttatcgatggagttggtatgcaggcacattacagcttatcaacaaatcctaccaatgttgagctttcattggagagatttatttctctgggtgttgaagtaagcataaccgagcttgatgtacaggcaggcagtgacttcaagttaactgatgaaattgcagaagcacagggatatttatacgcacagttatttgatatatataagaaacatgcagctaacataagccgtgttactatttggggattggatgacggcacaagctggagatcatctacaaatcctttgccatttgacaagaatcttcaggcaaagctcgcatttttcggtttaatagaccctgctaagtacatggatgaacat BBa_K392036_sequence 1 aagaaatgatggtaaatgaaataggaaatcaaggagcatgaaggcaaaagacaaatataagggtcgaacgaaaaataaagtgaaaagtgttgatatgatgtatttggctttgcggcgccgaaaaaacgagtttacgcaattgcacaatcatgctgactctgtggcggacccgcgctcttgccggcccggcgataacgctgggcgtgaggctgtgcccggcggagttttttgcgcctgcattttccaaggtttaccctgcgctaaggggcgagattggagaagcaataagaatgccggttggggttgcgatgatgacgaccacgacaactggtgtcattatttaagttgccgaaagaacctgagtgcatttgcaacatgagtatactagaagaatgagccaagacttgcgagacgcgagtttgccggtggtgcgaacaatagagcgaccatgaccttgaaggtgagacgcgcataaccgctagagtactttgaagaggaaacagcaatagggttgctaccagtataaatagacaggtacatacaacactggaaatggttgtctgtttgagtacgctttcaattcatttgggtgtgcactttattatgttacaatatggaagggaactttacacttctcctatgcacatatattaattaaagtccaatgctagtagagaaggggggtaacacccctccgcgctcttttccgatttttttctaaaccgtggaatatttcggatatccttttgttgtttccgggtgtacaatatggacttcctcttttctggcaaccaaacccatacatcgggattcctataataccttcgttggtctccctaacatgtaggtggcggaggggagatatacaatagaacagataccagacaagacataatgggctaaacaagactacaccaattacactgcctcattgatggtggtacataacgaactaatactgtagccctagacttgatagccatcatcatatcgaagtttcactaccctttttccatttgccatctattgaagtaataataggcgcatgcaacttcttttctttttttttcttttctctctcccccgttgttgtctcaccatatccgcaatgacaaaaaaaatgatggaagacactaaaggaaaaaattaacgacaaagacagcaccaacagatgtcgttgttccagagctgatgaggggtatcttcgaacacacgaaactttttccttccttcattcacgcacactactctctaatgagcaacggtatacggccttccttccagttacttgaatttgaaataaaaaaagtttgccgctttgctatcaagtataaatagacctgcaattattaatcttttgtttcctcgtcattgttctcgttccctttcttccttgtttctttttctgcacaatatttcaagctataccaagcatacaatcaacttactagatgaaagttttgattgttttgttggctattttcgctgctttgccattggctttggctcaaccagttatttctactactgttggttctgctgctgaaggttcactagataaaagatactagagtccacccgcagaaccgccgcagcgctgctggcggccgcggccgtcgccgtcggcggtctgaccgccctcaccaccaccgccgcgcaggcggctcccggctgccgcgtcgactacgccgtcaccaaccagtggcccggcggcttcggcgccaacgtcacgatcaccaacctcggcgaccccgtctcgtcgtggaagctcgactggacctacaccgcaggccagcggatccagcagctgtggaacggcaccgcgtcgaccaacggcggccaggtctccgtcaccagcctgccctggaacggcagcatcccgaccggcggcacggcgtcgttcgggttcaacggctcgtgggccgggtccaacccgacgccggcgtcgttctcgctcaacggcaccacctgcacgggcaccgtgccgacgaccagccccacgccgaccccgacgccgacgacccccacgccgacgccgaccccgacccccacccccacgccgacggtcacgccgcagccgaccagcggcttctacgtcgacccgacgacgcagggctaccgcgcgtggcaggccgcgtccggcacggacaaggcgctgctcgagaagatcgcgctcaccccgcaggcgtactgggtcggcaactgggccgacgcgtcgcacgcgcaggccgaggtcgccgactacaccggccgcgccgtcgcggccgggaagacgccgatgctcgtcgtctacgcgatcccgggccgcgactgcggctcgcactccggcggtggtgtgtccgagtccgagtacgcgcgctgggtcgacaccgtcgcgcagggcatcaagggcaacccgatcgtgatcctcgagcccgacgcgctcgcgcagctcggcgactgctccggccagggtgaccgcgtcggcttcctcaagtacgccgccaagtcgctcaccctcaagggcgcgcgcgtctacatcgacgcgggccacgcgaagtggctgtcggtcgacacgccggtgaaccgcctcaaccaggtcggcttcgagtacgcggtgggcttcgcgctcaacacgtcgaactaccagacgacggcggacagcaaggcgtacggccagcagatctcgcagcggctgggcggcaagaagttcgtcatcgacacctcgcgcaacggcaacggctcgaacggcgagtggtgcaacccgcgcggccgcgcgctcggcgaacgcccggtcgcggtgaacgacggctccggcctggacgcgctcctgtgggtcaagctgcccggcgagtccgacggcgcgtgcaacggcggcccggccgccggccagtggtggcaggagatcgccctggagatggcgcgcaacgccaggtggtactagagattgacgatattagctttgaacaaacgggttcaggaccaatcaaaatccaagatgatttaaaatccattaaagatgtatataaaaaggatttccttattggaaacgcaatagcagcagaggatatggaaggggtcagacttgacctgttaaagaagcactttaatgtaatgacagctggtaatgctatgaagccagatgcattacaaccttcaaagggtaactttacatttgaagatgcagataagcttgttgataaggttctggcagagggattcaagatgcatggacatactcttgtttggcatcagcagtcacctgcttggttaaatacaaaagttgatgcaagcggaaatgctatacctttgtcacgtgaggaagctcttacaaatctaagaacccatattaagacagttgtagagcactacggtaacaaggtaatatcatgggacgtagtaaatgaaggtatgaatgataatcctactaatcctgctgactggaaagcatcattacgtcaaagcccatggtatcaggcaattggtcctgattacatagaagaggcgtttttagcagcaagagaggttctggatgctcaccctgattgggatgtgaagctatattataatgattataacgatgataatcaaagcaaatctaaagctatttactacatggtaaaagagttaaatgaaaaatatgcaaaaactcaccctggtaagcttcttatcgatggagttggtatgcaggcacattacagcttatcaacaaatcctaccaatgttgagctttcattggagagatttatttctctgggtgttgaagtaagcataaccgagcttgatgtacaggcaggcagtgacttcaagttaactgatgaaattgcagaagcacagggatatttatacgcacagttatttgatatatataagaaacatgcagctaacataagccgtgttactatttggggattggatgacggcacaagctggagatcatctacaaatcctttgccatttgacaagaatcttcaggcaaagctcgcatttttcggtttaatagaccctgctaagtacatggatgaacattactagaggcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctattactagagcccgccgccaccatggag BBa_K416003_sequence 1 atgaaagttttgattgttttgttggctattttcgctgctttgccattggctttggctcaaccagttatttctactactgttggttctgctgctgaaggttcactagataaaaga BBa_J63003_sequence 1 cccgccgccaccatggag BBa_K392003_sequence 1 gcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctattactagagcccgccgccaccatggag BBa_J63005_sequence 1 aagaaatgatggtaaatgaaataggaaatcaaggagcatgaaggcaaaagacaaatataagggtcgaacgaaaaataaagtgaaaagtgttgatatgatgtatttggctttgcggcgccgaaaaaacgagtttacgcaattgcacaatcatgctgactctgtggcggacccgcgctcttgccggcccggcgataacgctgggcgtgaggctgtgcccggcggagttttttgcgcctgcattttccaaggtttaccctgcgctaaggggcgagattggagaagcaataagaatgccggttggggttgcgatgatgacgaccacgacaactggtgtcattatttaagttgccgaaagaacctgagtgcatttgcaacatgagtatactagaagaatgagccaagacttgcgagacgcgagtttgccggtggtgcgaacaatagagcgaccatgaccttgaaggtgagacgcgcataaccgctagagtactttgaagaggaaacagcaatagggttgctaccagtataaatagacaggtacatacaacactggaaatggttgtctgtttgagtacgctttcaattcatttgggtgtgcactttattatgttacaatatggaagggaactttacacttctcctatgcacatatattaattaaagtccaatgctagtagagaaggggggtaacacccctccgcgctcttttccgatttttttctaaaccgtggaatatttcggatatccttttgttgtttccgggtgtacaatatggacttcctcttttctggcaaccaaacccatacatcgggattcctataataccttcgttggtctccctaacatgtaggtggcggaggggagatatacaatagaacagataccagacaagacataatgggctaaacaagactacaccaattacactgcctcattgatggtggtacataacgaactaatactgtagccctagacttgatagccatcatcatatcgaagtttcactaccctttttccatttgccatctattgaagtaataataggcgcatgcaacttcttttctttttttttcttttctctctcccccgttgttgtctcaccatatccgcaatgacaaaaaaaatgatggaagacactaaaggaaaaaattaacgacaaagacagcaccaacagatgtcgttgttccagagctgatgaggggtatcttcgaacacacgaaactttttccttccttcattcacgcacactactctctaatgagcaacggtatacggccttccttccagttacttgaatttgaaataaaaaaagtttgccgctttgctatcaagtataaatagacctgcaattattaatcttttgtttcctcgtcattgttctcgttccctttcttccttgtttctttttctgcacaatatttcaagctataccaagcatacaatcaact BBa_K105027_sequence 1 gcatgtgctctgtatgtatataaaactcttgttttcttcttttctctaaatattctttccttatacattaggacctttgcagcataaattactatacttctat igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z