LOCUS       BBa_K392037             3362 bp    DNA     linear       03-May-2025
DEFINITION  exoglucanase surface display testing construct for yeast
ACCESSION   BBa_K392037
VERSION     BBa_K392037.1  
FEATURES             Location/Qualifiers
     promoter        1..1445
                     /label=Constitutive promoter
     misc_feature    1348..1354
                     /label=TATA Box
     promoter        1..1445
                     /label=BBa_J63005
     CDS             1452..1712
                     /label=Aga2 CDS
     misc_feature    1452..1712
                     /label=BBa_K416000
     misc_feature    1721..1765
                     /label=BBa_K416001
     misc_feature    1452..1765
                     /label=BBa_K416004
     misc_feature    1774..3225
                     /label=BBa_K392007
     misc_feature    3250..3256
                     /label=TATA box
     promoter        3234..3336
                     /label=BBa_K105027
     misc_feature    3357..3359
                     /label=start codon
     RBS             3345..3362
                     /label=BBa_J63003
     terminator      3234..3362
                     /label=BBa_K392003
ORIGIN
        1 aagaaatgat ggtaaatgaa ataggaaatc aaggagcatg aaggcaaaag acaaatataa
       61 gggtcgaacg aaaaataaag tgaaaagtgt tgatatgatg tatttggctt tgcggcgccg
      121 aaaaaacgag tttacgcaat tgcacaatca tgctgactct gtggcggacc cgcgctcttg
      181 ccggcccggc gataacgctg ggcgtgaggc tgtgcccggc ggagtttttt gcgcctgcat
      241 tttccaaggt ttaccctgcg ctaaggggcg agattggaga agcaataaga atgccggttg
      301 gggttgcgat gatgacgacc acgacaactg gtgtcattat ttaagttgcc gaaagaacct
      361 gagtgcattt gcaacatgag tatactagaa gaatgagcca agacttgcga gacgcgagtt
      421 tgccggtggt gcgaacaata gagcgaccat gaccttgaag gtgagacgcg cataaccgct
      481 agagtacttt gaagaggaaa cagcaatagg gttgctacca gtataaatag acaggtacat
      541 acaacactgg aaatggttgt ctgtttgagt acgctttcaa ttcatttggg tgtgcacttt
      601 attatgttac aatatggaag ggaactttac acttctccta tgcacatata ttaattaaag
      661 tccaatgcta gtagagaagg ggggtaacac ccctccgcgc tcttttccga tttttttcta
      721 aaccgtggaa tatttcggat atccttttgt tgtttccggg tgtacaatat ggacttcctc
      781 ttttctggca accaaaccca tacatcggga ttcctataat accttcgttg gtctccctaa
      841 catgtaggtg gcggagggga gatatacaat agaacagata ccagacaaga cataatgggc
      901 taaacaagac tacaccaatt acactgcctc attgatggtg gtacataacg aactaatact
      961 gtagccctag acttgatagc catcatcata tcgaagtttc actacccttt ttccatttgc
     1021 catctattga agtaataata ggcgcatgca acttcttttc tttttttttc ttttctctct
     1081 cccccgttgt tgtctcacca tatccgcaat gacaaaaaaa atgatggaag acactaaagg
     1141 aaaaaattaa cgacaaagac agcaccaaca gatgtcgttg ttccagagct gatgaggggt
     1201 atcttcgaac acacgaaact ttttccttcc ttcattcacg cacactactc tctaatgagc
     1261 aacggtatac ggccttcctt ccagttactt gaatttgaaa taaaaaaagt ttgccgcttt
     1321 gctatcaagt ataaatagac ctgcaattat taatcttttg tttcctcgtc attgttctcg
     1381 ttccctttct tccttgtttc tttttctgca caatatttca agctatacca agcatacaat
     1441 caacttacta gatgcagtta cttcgctgtt tttcaatatt ttctgttatt gcttcagttt
     1501 tagcacagga actgacaact atatgcgagc aaatcccctc accaacttta gaatcgacgc
     1561 cgtactcttt gtcaacgact actattttgg ccaacgggaa ggcaatgcaa ggagtttttg
     1621 aatattacaa atcagtaacg tttgtcagta attgcggttc tcacccctca acaactagca
     1681 aaggcagccc cataaacaca cagtatgttt tttactagag ggtggaggag gctctggtgg
     1741 aggcggtagc ggaggcggag ggtcgtacta gagcctagga ccacgcccgc acccggccac
     1801 ccggcccgcg gcgcccgcac cgctctgcgc acgacgctcg ccgccgcggc ggcgacgctc
     1861 gtcgtcggcg ccacggtcgt gctgcccgcc caggccgcga ccacgctcaa ggaggccgcc
     1921 gacggcgccg gccgggactt cggcttcgcg ctcgacccca accggctctc ggaggcgcag
     1981 tacaaggcga tcgccgacag cgagttcaac ctcgtcgtcg ccgagaacgc gatgaagtgg
     2041 gacgccaccg agccctcgca gaacagcttc tccttcggcg cgggcgaccg cgtcgcgagc
     2101 tacgccgccg acaccggcaa ggagctgtac ggccacacgc tcgtatggca ctcgcagctg
     2161 cccgactggg cgaagaacct caacggctcc gcgttcgaga gcgcgatggt caaccacgtg
     2221 acgaaggtcg ccgaccactt cgagggcaag gtcgcgtcgt gggacgtcgt caacgaggcg
     2281 ttcgccgacg gcggcggccg ccggcaggac tcggcgttcc agcagaagct cggcaacggc
     2341 tacatcgaga ccgcgttccg ggcggcacgt gcggcggacc cgaccgccaa gctgtgcatc
     2401 aacgactaca acgtcgaggg catcaacgcg aagagcaact cgctctacga cctcgtcaag
     2461 gacttcaagg cgcgcggcgt cccgctcgac tgcgtcgggt tccagtcgca cctcatcgtc
     2521 ggccaggtgc cgggcgactt ccggcagaac ctgcaacggt tcgcggacct gggcgtggac
     2581 gtgcgcatca ccgagctcga catccgcatg cggacgccct ccgacgcgac caagctcgcg
     2641 acccaggcgg ccgactacaa gaaggtcgtg caggcctgca tgcaggtgac ccgctgccag
     2701 ggcgtgaccg tctggggcat caccgacaag tactcgtggg tgccggacgt cttcccgggc
     2761 gagggggccg cgctggtgtg ggacgcgagc tacgccaaga agccggccta cgccgccgtg
     2821 atggaggcct tcggcgcgag cccgacgccg acgcccacca cgccgacccc gacgcccacg
     2881 acgccgacgc cgaccccgac gtccggtccg gccgggtgcc aggtgctgtg gggcgtcaac
     2941 cagtggaaca ccggcttcac cgcgaacgtc accgtgaaga acacgtcctc cgctccggtc
     3001 gacggctgga cgctcacgtt cagcttcccg tccggccagc aggtcaccca ggcgtggagc
     3061 tcgacggtca cgcagtccgg ctcggccgtg acggtccgca acgccccgtg gaacggctcg
     3121 atcccggcgg gcggcaccgc gcagttcggc ttcaacggct cgcacacggg caccaacgcc
     3181 gcgccgacgg cgttctcgct caacggcacg ccctgcacgg tcggctacta gaggcatgtg
     3241 ctctgtatgt atataaaact cttgttttct tcttttctct aaatattctt tccttataca
     3301 ttaggacctt tgcagcataa attactatac ttctattact agagcccgcc gccaccatgg
     3361 ag
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