LOCUS       BBa_K392039             1732 bp    DNA     linear       22-Dec-2024
DEFINITION  endoglucanase secretion testing construct for E. coli
ACCESSION   BBa_K392039
VERSION     BBa_K392039.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..292
                     /label=PelB
     misc_feature    227..292
                     /label=BBa_J32015
     misc_feature    301..1644
                     /label=BBa_K392006
     stem_loop       1664..1707
                     /label=stem_loop
     misc_feature    1653..1732
                     /label=BBa_B0010
     terminator      1653..1732
                     /label=BBa_B0010
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga aatacctgct
      241 gccgaccgct gctgctggtc tgctgctcct cgctgcccag ccggcgatgg cctactagag
      301 tccacccgca gaaccgccgc agcgctgctg gcggccgcgg ccgtcgccgt cggcggtctg
      361 accgccctca ccaccaccgc cgcgcaggcg gctcccggct gccgcgtcga ctacgccgtc
      421 accaaccagt ggcccggcgg cttcggcgcc aacgtcacga tcaccaacct cggcgacccc
      481 gtctcgtcgt ggaagctcga ctggacctac accgcaggcc agcggatcca gcagctgtgg
      541 aacggcaccg cgtcgaccaa cggcggccag gtctccgtca ccagcctgcc ctggaacggc
      601 agcatcccga ccggcggcac ggcgtcgttc gggttcaacg gctcgtgggc cgggtccaac
      661 ccgacgccgg cgtcgttctc gctcaacggc accacctgca cgggcaccgt gccgacgacc
      721 agccccacgc cgaccccgac gccgacgacc cccacgccga cgccgacccc gacccccacc
      781 cccacgccga cggtcacgcc gcagccgacc agcggcttct acgtcgaccc gacgacgcag
      841 ggctaccgcg cgtggcaggc cgcgtccggc acggacaagg cgctgctcga gaagatcgcg
      901 ctcaccccgc aggcgtactg ggtcggcaac tgggccgacg cgtcgcacgc gcaggccgag
      961 gtcgccgact acaccggccg cgccgtcgcg gccgggaaga cgccgatgct cgtcgtctac
     1021 gcgatcccgg gccgcgactg cggctcgcac tccggcggtg gtgtgtccga gtccgagtac
     1081 gcgcgctggg tcgacaccgt cgcgcagggc atcaagggca acccgatcgt gatcctcgag
     1141 cccgacgcgc tcgcgcagct cggcgactgc tccggccagg gtgaccgcgt cggcttcctc
     1201 aagtacgccg ccaagtcgct caccctcaag ggcgcgcgcg tctacatcga cgcgggccac
     1261 gcgaagtggc tgtcggtcga cacgccggtg aaccgcctca accaggtcgg cttcgagtac
     1321 gcggtgggct tcgcgctcaa cacgtcgaac taccagacga cggcggacag caaggcgtac
     1381 ggccagcaga tctcgcagcg gctgggcggc aagaagttcg tcatcgacac ctcgcgcaac
     1441 ggcaacggct cgaacggcga gtggtgcaac ccgcgcggcc gcgcgctcgg cgaacgcccg
     1501 gtcgcggtga acgacggctc cggcctggac gcgctcctgt gggtcaagct gcccggcgag
     1561 tccgacggcg cgtgcaacgg cggcccggcc gccggccagt ggtggcagga gatcgccctg
     1621 gagatggcgc gcaacgccag gtggtactag agccaggcat caaataaaac gaaaggctca
     1681 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tc
//