LOCUS       BBa_K392040             2823 bp    DNA     linear       22-Dec-2024
DEFINITION  endoglucanase/CBM secretion testing construct for E. coli
ACCESSION   BBa_K392040
VERSION     BBa_K392040.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..292
                     /label=PelB
     misc_feature    227..292
                     /label=BBa_J32015
     misc_feature    301..1644
                     /label=BBa_K392006
     misc_feature    1653..2735
                     /label=BBa_K392014
     stem_loop       2755..2798
                     /label=stem_loop
     misc_feature    2744..2823
                     /label=BBa_B0010
     terminator      2744..2823
                     /label=BBa_B0010
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga aatacctgct
      241 gccgaccgct gctgctggtc tgctgctcct cgctgcccag ccggcgatgg cctactagag
      301 tccacccgca gaaccgccgc agcgctgctg gcggccgcgg ccgtcgccgt cggcggtctg
      361 accgccctca ccaccaccgc cgcgcaggcg gctcccggct gccgcgtcga ctacgccgtc
      421 accaaccagt ggcccggcgg cttcggcgcc aacgtcacga tcaccaacct cggcgacccc
      481 gtctcgtcgt ggaagctcga ctggacctac accgcaggcc agcggatcca gcagctgtgg
      541 aacggcaccg cgtcgaccaa cggcggccag gtctccgtca ccagcctgcc ctggaacggc
      601 agcatcccga ccggcggcac ggcgtcgttc gggttcaacg gctcgtgggc cgggtccaac
      661 ccgacgccgg cgtcgttctc gctcaacggc accacctgca cgggcaccgt gccgacgacc
      721 agccccacgc cgaccccgac gccgacgacc cccacgccga cgccgacccc gacccccacc
      781 cccacgccga cggtcacgcc gcagccgacc agcggcttct acgtcgaccc gacgacgcag
      841 ggctaccgcg cgtggcaggc cgcgtccggc acggacaagg cgctgctcga gaagatcgcg
      901 ctcaccccgc aggcgtactg ggtcggcaac tgggccgacg cgtcgcacgc gcaggccgag
      961 gtcgccgact acaccggccg cgccgtcgcg gccgggaaga cgccgatgct cgtcgtctac
     1021 gcgatcccgg gccgcgactg cggctcgcac tccggcggtg gtgtgtccga gtccgagtac
     1081 gcgcgctggg tcgacaccgt cgcgcagggc atcaagggca acccgatcgt gatcctcgag
     1141 cccgacgcgc tcgcgcagct cggcgactgc tccggccagg gtgaccgcgt cggcttcctc
     1201 aagtacgccg ccaagtcgct caccctcaag ggcgcgcgcg tctacatcga cgcgggccac
     1261 gcgaagtggc tgtcggtcga cacgccggtg aaccgcctca accaggtcgg cttcgagtac
     1321 gcggtgggct tcgcgctcaa cacgtcgaac taccagacga cggcggacag caaggcgtac
     1381 ggccagcaga tctcgcagcg gctgggcggc aagaagttcg tcatcgacac ctcgcgcaac
     1441 ggcaacggct cgaacggcga gtggtgcaac ccgcgcggcc gcgcgctcgg cgaacgcccg
     1501 gtcgcggtga acgacggctc cggcctggac gcgctcctgt gggtcaagct gcccggcgag
     1561 tccgacggcg cgtgcaacgg cggcccggcc gccggccagt ggtggcagga gatcgccctg
     1621 gagatggcgc gcaacgccag gtggtactag agattgacga tattagcttt gaacaaacgg
     1681 gttcaggacc aatcaaaatc caagatgatt taaaatccat taaagatgta tataaaaagg
     1741 atttccttat tggaaacgca atagcagcag aggatatgga aggggtcaga cttgacctgt
     1801 taaagaagca ctttaatgta atgacagctg gtaatgctat gaagccagat gcattacaac
     1861 cttcaaaggg taactttaca tttgaagatg cagataagct tgttgataag gttctggcag
     1921 agggattcaa gatgcatgga catactcttg tttggcatca gcagtcacct gcttggttaa
     1981 atacaaaagt tgatgcaagc ggaaatgcta tacctttgtc acgtgaggaa gctcttacaa
     2041 atctaagaac ccatattaag acagttgtag agcactacgg taacaaggta atatcatggg
     2101 acgtagtaaa tgaaggtatg aatgataatc ctactaatcc tgctgactgg aaagcatcat
     2161 tacgtcaaag cccatggtat caggcaattg gtcctgatta catagaagag gcgtttttag
     2221 cagcaagaga ggttctggat gctcaccctg attgggatgt gaagctatat tataatgatt
     2281 ataacgatga taatcaaagc aaatctaaag ctatttacta catggtaaaa gagttaaatg
     2341 aaaaatatgc aaaaactcac cctggtaagc ttcttatcga tggagttggt atgcaggcac
     2401 attacagctt atcaacaaat cctaccaatg ttgagctttc attggagaga tttatttctc
     2461 tgggtgttga agtaagcata accgagcttg atgtacaggc aggcagtgac ttcaagttaa
     2521 ctgatgaaat tgcagaagca cagggatatt tatacgcaca gttatttgat atatataaga
     2581 aacatgcagc taacataagc cgtgttacta tttggggatt ggatgacggc acaagctgga
     2641 gatcatctac aaatcctttg ccatttgaca agaatcttca ggcaaagctc gcatttttcg
     2701 gtttaataga ccctgctaag tacatggatg aacattacta gagccaggca tcaaataaaa
     2761 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     2821 ctc
//