LOCUS       BBa_K392042             2931 bp    DNA     linear       22-Nov-2024
DEFINITION  exoglucanase/CBM secretion testing construct for E. coli
ACCESSION   BBa_K392042
VERSION     BBa_K392042.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..292
                     /label=PelB
     misc_feature    227..292
                     /label=BBa_J32015
     misc_feature    301..1752
                     /label=BBa_K392007
     misc_feature    1761..2843
                     /label=BBa_K392014
     stem_loop       2863..2906
                     /label=stem_loop
     misc_feature    2852..2931
                     /label=BBa_B0010
     terminator      2852..2931
                     /label=BBa_B0010
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga aatacctgct
      241 gccgaccgct gctgctggtc tgctgctcct cgctgcccag ccggcgatgg cctactagag
      301 cctaggacca cgcccgcacc cggccacccg gcccgcggcg cccgcaccgc tctgcgcacg
      361 acgctcgccg ccgcggcggc gacgctcgtc gtcggcgcca cggtcgtgct gcccgcccag
      421 gccgcgacca cgctcaagga ggccgccgac ggcgccggcc gggacttcgg cttcgcgctc
      481 gaccccaacc ggctctcgga ggcgcagtac aaggcgatcg ccgacagcga gttcaacctc
      541 gtcgtcgccg agaacgcgat gaagtgggac gccaccgagc cctcgcagaa cagcttctcc
      601 ttcggcgcgg gcgaccgcgt cgcgagctac gccgccgaca ccggcaagga gctgtacggc
      661 cacacgctcg tatggcactc gcagctgccc gactgggcga agaacctcaa cggctccgcg
      721 ttcgagagcg cgatggtcaa ccacgtgacg aaggtcgccg accacttcga gggcaaggtc
      781 gcgtcgtggg acgtcgtcaa cgaggcgttc gccgacggcg gcggccgccg gcaggactcg
      841 gcgttccagc agaagctcgg caacggctac atcgagaccg cgttccgggc ggcacgtgcg
      901 gcggacccga ccgccaagct gtgcatcaac gactacaacg tcgagggcat caacgcgaag
      961 agcaactcgc tctacgacct cgtcaaggac ttcaaggcgc gcggcgtccc gctcgactgc
     1021 gtcgggttcc agtcgcacct catcgtcggc caggtgccgg gcgacttccg gcagaacctg
     1081 caacggttcg cggacctggg cgtggacgtg cgcatcaccg agctcgacat ccgcatgcgg
     1141 acgccctccg acgcgaccaa gctcgcgacc caggcggccg actacaagaa ggtcgtgcag
     1201 gcctgcatgc aggtgacccg ctgccagggc gtgaccgtct ggggcatcac cgacaagtac
     1261 tcgtgggtgc cggacgtctt cccgggcgag ggggccgcgc tggtgtggga cgcgagctac
     1321 gccaagaagc cggcctacgc cgccgtgatg gaggccttcg gcgcgagccc gacgccgacg
     1381 cccaccacgc cgaccccgac gcccacgacg ccgacgccga ccccgacgtc cggtccggcc
     1441 gggtgccagg tgctgtgggg cgtcaaccag tggaacaccg gcttcaccgc gaacgtcacc
     1501 gtgaagaaca cgtcctccgc tccggtcgac ggctggacgc tcacgttcag cttcccgtcc
     1561 ggccagcagg tcacccaggc gtggagctcg acggtcacgc agtccggctc ggccgtgacg
     1621 gtccgcaacg ccccgtggaa cggctcgatc ccggcgggcg gcaccgcgca gttcggcttc
     1681 aacggctcgc acacgggcac caacgccgcg ccgacggcgt tctcgctcaa cggcacgccc
     1741 tgcacggtcg gctactagag attgacgata ttagctttga acaaacgggt tcaggaccaa
     1801 tcaaaatcca agatgattta aaatccatta aagatgtata taaaaaggat ttccttattg
     1861 gaaacgcaat agcagcagag gatatggaag gggtcagact tgacctgtta aagaagcact
     1921 ttaatgtaat gacagctggt aatgctatga agccagatgc attacaacct tcaaagggta
     1981 actttacatt tgaagatgca gataagcttg ttgataaggt tctggcagag ggattcaaga
     2041 tgcatggaca tactcttgtt tggcatcagc agtcacctgc ttggttaaat acaaaagttg
     2101 atgcaagcgg aaatgctata cctttgtcac gtgaggaagc tcttacaaat ctaagaaccc
     2161 atattaagac agttgtagag cactacggta acaaggtaat atcatgggac gtagtaaatg
     2221 aaggtatgaa tgataatcct actaatcctg ctgactggaa agcatcatta cgtcaaagcc
     2281 catggtatca ggcaattggt cctgattaca tagaagaggc gtttttagca gcaagagagg
     2341 ttctggatgc tcaccctgat tgggatgtga agctatatta taatgattat aacgatgata
     2401 atcaaagcaa atctaaagct atttactaca tggtaaaaga gttaaatgaa aaatatgcaa
     2461 aaactcaccc tggtaagctt cttatcgatg gagttggtat gcaggcacat tacagcttat
     2521 caacaaatcc taccaatgtt gagctttcat tggagagatt tatttctctg ggtgttgaag
     2581 taagcataac cgagcttgat gtacaggcag gcagtgactt caagttaact gatgaaattg
     2641 cagaagcaca gggatattta tacgcacagt tatttgatat atataagaaa catgcagcta
     2701 acataagccg tgttactatt tggggattgg atgacggcac aagctggaga tcatctacaa
     2761 atcctttgcc atttgacaag aatcttcagg caaagctcgc atttttcggt ttaatagacc
     2821 ctgctaagta catggatgaa cattactaga gccaggcatc aaataaaacg aaaggctcag
     2881 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct c
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