BBa_J32015
1
PelB
PelB leader sequence; directs protein to E. coli periplasmic membrane
2006-08-24T11:00:00Z
2015-08-31T04:08:46Z
Novagen
Released HQ 2013
The pelB leader sequence is a sequence of amino acids which when attached to a protein, directs the protein to the periplasmic membrane of E. coli, where the sequence is removed by pelB peptidase. It is used to direct coat protein-antigen fusions to the cell surface.
false
true
_50_
0
495
50
In stock
true
.
true
Austen Heinz
annotation1898182
1
PelB
range1898182
1
1
66
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_R0010
1
LacI
promoter (lacI regulated)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
The Plac insert was PCR'd from the MG1655 strain of E.coli K12.
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG.
false
true
_1_
0
24
7
In stock
false
<P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs.
true
annotation1961227
1
start
range1961227
1
173
173
annotation1961221
1
end of LacI coding region (inactive)
range1961221
1
1
88
annotation1961225
1
-10
range1961225
1
161
166
annotation1961226
1
LacI binding site
range1961226
1
166
200
annotation1961223
1
CAP binding site
range1961223
1
89
126
annotation1961222
1
BBa_R0010
range1961222
1
1
200
annotation1961224
1
-35
range1961224
1
137
142
BBa_K392043
1
BBa_K392043
beta-glucosidase secretion testing construct for E. coli
2010-10-24T11:00:00Z
2015-05-08T01:12:21Z
Assembly of R0010, B0034, J32015, K392008, B0010.
Device containing C. fermi beta-glucosidase with E. coli PelB export tag attached upstream to enable secretion of protein.
false
false
_528_
0
6220
9
Not in stock
false
Assembly included Silver standard-compatible parts. Sequence shown on part's main page may not be accurate since it assumes Standard assembly. Please check sequencing results from Get This Part page instead.
false
Takuro A. Kagaya, Yuki Miyatake, Hirayama Ikumi
component2100202
1
BBa_R0010
component2100214
1
BBa_B0010
component2100210
1
BBa_B0034
component2100213
1
BBa_K392008
component2100212
1
BBa_J32015
annotation2100213
1
BBa_K392008
range2100213
1
299
1969
annotation2100212
1
BBa_J32015
range2100212
1
227
292
annotation2100202
1
BBa_R0010
range2100202
1
1
200
annotation2100214
1
BBa_B0010
range2100214
1
1978
2057
annotation2100210
1
BBa_B0034
range2100210
1
209
220
BBa_K392008
1
BBa_K392008
C. firmi beta-glucosidase (Silver standard compatible)
2010-10-23T11:00:00Z
2015-05-08T01:12:20Z
Original part received from Edinburgh team.
Beta-glucosidase from C. firmi re-cloned into Silver standard-compatible part.
false
false
_528_
0
6220
9
It's complicated
true
Cloned from original part (Edinburgh) by PCR.
false
Damian Barnard, Teoh Shao Thing, Tadashi Nakamura
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_R0010_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K392043_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggcctactagatgggcgaccggttccagcaggccggtcgcccacgccgccgcggcccggcgagggccgttaaccgtaccggtcaagaagacgcgtcgacggggtcgagggagcggtcccacgcgtgtatcgtatcgtttcgacaccgccacccggccaccgggcacgcaccggggacgcagcagtccccgccccggccaccccctgtcaccgaaacccgaaggaccctcatgaccaccacgcgcccctcgggccgccagttctccgacgacttcctgtggggctcggccacggcctcctaccagatcgagggcgcgcacgacgagggcggtcgcggcccgtcgatctgggacacgttctcccgcaccccgggcaaggtcctcaacggcgacaccggcgacgtcgcggtcgaccactaccaccgcgtccccgaggacgtcgagatcatgaagagcctcggcctccaggcctaccggttctcgatcgcctggccccgcatccagccgaccggctcgggcgagttcaaccaggccgggctcgacttctactccgacctcgtcgaccggctcatcgccgccggcatcaagcccgtcgcgacgctgtaccactgggacctcccgcagcccctggaggacgagggcggctgggcgaaccgcgcgacggcgtaccggttcgtcgagtacgcgcgcaagctcgccgaggtcctcggcaagcgcgtcgacctgtggaccacgctcaacgagccgtggtgctccgcgttcctcggctacgcctcaggcgtgcacgccccggcgtcaccgacccggtcaaggccctgcgcgccgtccaccacctcaacctcgcgcacggcctcgcgggccgcgcgatccgcgaggtcctcggcgagaacgcgcccgtctcgatcacgtgaacctgcacgtgacgcgcgccgccgacgactccgccgactccgtcgaggccaagcgccgcatcgacaccatcgcgaacgaggtcttcctcggcccgctgctcgacggcgagtaccccaaggaggtcttcgcggacaccgcgcaccacaccgactggtccttcgtcgagccgggcgacctcgagctcatccgcatcccgctcaccgtgctcggcgtcaactactactcgaccggccgcgtgaagaagggcagcgcgcgggcgccgacccgggcaagccgggccccgacggccaccgcgcctccgagcactcctcgtgggtcggcgccgacgaggtcgagtggctcccgcagcccggcccgcacaccgcgatgggctggaacatcgagcccgacggcctcgtcgacctgctgctcgagctgcgcgaccgttaccccagccagcccctggcgatcacggagaacggtgccgcgttctacgacacggtcagcgaggacggccgcgtgcacgaccccgagcgcgtcggctacctgcacgaccacgtcgacgccgtcggcgaggccatcgacaagggcgccgacgtgcgcgcctacttcgtgtggtcgctgctcgacaacttcgagtggcgctacggctacgaccgccggttcggcatcgtgcgcgtcgactacgacacgcacgagcgcatcgtgaaggactcgggcctctggtaccgcgagctcgtgcgcacccgcaccatcgctccggccgaggacgccgcgacctaccagccctgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_J32015_sequence
1
atgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggcc
BBa_K392008_sequence
1
atgggcgaccggttccagcaggccggtcgcccacgccgccgcggcccggcgagggccgttaaccgtaccggtcaagaagacgcgtcgacggggtcgagggagcggtcccacgcgtgtatcgtatcgtttcgacaccgccacccggccaccgggcacgcaccggggacgcagcagtccccgccccggccaccccctgtcaccgaaacccgaaggaccctcatgaccaccacgcgcccctcgggccgccagttctccgacgacttcctgtggggctcggccacggcctcctaccagatcgagggcgcgcacgacgagggcggtcgcggcccgtcgatctgggacacgttctcccgcaccccgggcaaggtcctcaacggcgacaccggcgacgtcgcggtcgaccactaccaccgcgtccccgaggacgtcgagatcatgaagagcctcggcctccaggcctaccggttctcgatcgcctggccccgcatccagccgaccggctcgggcgagttcaaccaggccgggctcgacttctactccgacctcgtcgaccggctcatcgccgccggcatcaagcccgtcgcgacgctgtaccactgggacctcccgcagcccctggaggacgagggcggctgggcgaaccgcgcgacggcgtaccggttcgtcgagtacgcgcgcaagctcgccgaggtcctcggcaagcgcgtcgacctgtggaccacgctcaacgagccgtggtgctccgcgttcctcggctacgcctcaggcgtgcacgccccggcgtcaccgacccggtcaaggccctgcgcgccgtccaccacctcaacctcgcgcacggcctcgcgggccgcgcgatccgcgaggtcctcggcgagaacgcgcccgtctcgatcacgtgaacctgcacgtgacgcgcgccgccgacgactccgccgactccgtcgaggccaagcgccgcatcgacaccatcgcgaacgaggtcttcctcggcccgctgctcgacggcgagtaccccaaggaggtcttcgcggacaccgcgcaccacaccgactggtccttcgtcgagccgggcgacctcgagctcatccgcatcccgctcaccgtgctcggcgtcaactactactcgaccggccgcgtgaagaagggcagcgcgcgggcgccgacccgggcaagccgggccccgacggccaccgcgcctccgagcactcctcgtgggtcggcgccgacgaggtcgagtggctcccgcagcccggcccgcacaccgcgatgggctggaacatcgagcccgacggcctcgtcgacctgctgctcgagctgcgcgaccgttaccccagccagcccctggcgatcacggagaacggtgccgcgttctacgacacggtcagcgaggacggccgcgtgcacgaccccgagcgcgtcggctacctgcacgaccacgtcgacgccgtcggcgaggccatcgacaagggcgccgacgtgcgcgcctacttcgtgtggtcgctgctcgacaacttcgagtggcgctacggctacgaccgccggttcggcatcgtgcgcgtcgactacgacacgcacgagcgcatcgtgaaggactcgggcctctggtaccgcgagctcgtgcgcacccgcaccatcgctccggccgaggacgccgcgacctaccagccctga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z