LOCUS       BBa_K392044             3148 bp    DNA     linear       23-Nov-2024
DEFINITION  beta-glucosidase/CBM secretion testing construct for E. coli
ACCESSION   BBa_K392044
VERSION     BBa_K392044.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..292
                     /label=PelB
     misc_feature    227..292
                     /label=BBa_J32015
     misc_feature    299..1969
                     /label=BBa_K392008
     misc_feature    1978..3060
                     /label=BBa_K392014
     stem_loop       3080..3123
                     /label=stem_loop
     misc_feature    3069..3148
                     /label=BBa_B0010
     terminator      3069..3148
                     /label=BBa_B0010
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga aatacctgct
      241 gccgaccgct gctgctggtc tgctgctcct cgctgcccag ccggcgatgg cctactagat
      301 gggcgaccgg ttccagcagg ccggtcgccc acgccgccgc ggcccggcga gggccgttaa
      361 ccgtaccggt caagaagacg cgtcgacggg gtcgagggag cggtcccacg cgtgtatcgt
      421 atcgtttcga caccgccacc cggccaccgg gcacgcaccg gggacgcagc agtccccgcc
      481 ccggccaccc cctgtcaccg aaacccgaag gaccctcatg accaccacgc gcccctcggg
      541 ccgccagttc tccgacgact tcctgtgggg ctcggccacg gcctcctacc agatcgaggg
      601 cgcgcacgac gagggcggtc gcggcccgtc gatctgggac acgttctccc gcaccccggg
      661 caaggtcctc aacggcgaca ccggcgacgt cgcggtcgac cactaccacc gcgtccccga
      721 ggacgtcgag atcatgaaga gcctcggcct ccaggcctac cggttctcga tcgcctggcc
      781 ccgcatccag ccgaccggct cgggcgagtt caaccaggcc gggctcgact tctactccga
      841 cctcgtcgac cggctcatcg ccgccggcat caagcccgtc gcgacgctgt accactggga
      901 cctcccgcag cccctggagg acgagggcgg ctgggcgaac cgcgcgacgg cgtaccggtt
      961 cgtcgagtac gcgcgcaagc tcgccgaggt cctcggcaag cgcgtcgacc tgtggaccac
     1021 gctcaacgag ccgtggtgct ccgcgttcct cggctacgcc tcaggcgtgc acgccccggc
     1081 gtcaccgacc cggtcaaggc cctgcgcgcc gtccaccacc tcaacctcgc gcacggcctc
     1141 gcgggccgcg cgatccgcga ggtcctcggc gagaacgcgc ccgtctcgat cacgtgaacc
     1201 tgcacgtgac gcgcgccgcc gacgactccg ccgactccgt cgaggccaag cgccgcatcg
     1261 acaccatcgc gaacgaggtc ttcctcggcc cgctgctcga cggcgagtac cccaaggagg
     1321 tcttcgcgga caccgcgcac cacaccgact ggtccttcgt cgagccgggc gacctcgagc
     1381 tcatccgcat cccgctcacc gtgctcggcg tcaactacta ctcgaccggc cgcgtgaaga
     1441 agggcagcgc gcgggcgccg acccgggcaa gccgggcccc gacggccacc gcgcctccga
     1501 gcactcctcg tgggtcggcg ccgacgaggt cgagtggctc ccgcagcccg gcccgcacac
     1561 cgcgatgggc tggaacatcg agcccgacgg cctcgtcgac ctgctgctcg agctgcgcga
     1621 ccgttacccc agccagcccc tggcgatcac ggagaacggt gccgcgttct acgacacggt
     1681 cagcgaggac ggccgcgtgc acgaccccga gcgcgtcggc tacctgcacg accacgtcga
     1741 cgccgtcggc gaggccatcg acaagggcgc cgacgtgcgc gcctacttcg tgtggtcgct
     1801 gctcgacaac ttcgagtggc gctacggcta cgaccgccgg ttcggcatcg tgcgcgtcga
     1861 ctacgacacg cacgagcgca tcgtgaagga ctcgggcctc tggtaccgcg agctcgtgcg
     1921 cacccgcacc atcgctccgg ccgaggacgc cgcgacctac cagccctgat actagagatt
     1981 gacgatatta gctttgaaca aacgggttca ggaccaatca aaatccaaga tgatttaaaa
     2041 tccattaaag atgtatataa aaaggatttc cttattggaa acgcaatagc agcagaggat
     2101 atggaagggg tcagacttga cctgttaaag aagcacttta atgtaatgac agctggtaat
     2161 gctatgaagc cagatgcatt acaaccttca aagggtaact ttacatttga agatgcagat
     2221 aagcttgttg ataaggttct ggcagaggga ttcaagatgc atggacatac tcttgtttgg
     2281 catcagcagt cacctgcttg gttaaataca aaagttgatg caagcggaaa tgctatacct
     2341 ttgtcacgtg aggaagctct tacaaatcta agaacccata ttaagacagt tgtagagcac
     2401 tacggtaaca aggtaatatc atgggacgta gtaaatgaag gtatgaatga taatcctact
     2461 aatcctgctg actggaaagc atcattacgt caaagcccat ggtatcaggc aattggtcct
     2521 gattacatag aagaggcgtt tttagcagca agagaggttc tggatgctca ccctgattgg
     2581 gatgtgaagc tatattataa tgattataac gatgataatc aaagcaaatc taaagctatt
     2641 tactacatgg taaaagagtt aaatgaaaaa tatgcaaaaa ctcaccctgg taagcttctt
     2701 atcgatggag ttggtatgca ggcacattac agcttatcaa caaatcctac caatgttgag
     2761 ctttcattgg agagatttat ttctctgggt gttgaagtaa gcataaccga gcttgatgta
     2821 caggcaggca gtgacttcaa gttaactgat gaaattgcag aagcacaggg atatttatac
     2881 gcacagttat ttgatatata taagaaacat gcagctaaca taagccgtgt tactatttgg
     2941 ggattggatg acggcacaag ctggagatca tctacaaatc ctttgccatt tgacaagaat
     3001 cttcaggcaa agctcgcatt tttcggttta atagaccctg ctaagtacat ggatgaacat
     3061 tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     3121 tatctgttgt ttgtcggtga acgctctc
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