BBa_R0010 1 LacI promoter (lacI regulated) 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z The Plac insert was PCR'd from the MG1655 strain of E.coli K12. Released HQ 2013 Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The pLac regulatory region is a 243 base-pair sequence with standard BioBrick prefix and suffix sections on its ends. It contains two protein binding sites: CAP, which is generally present in E.coli and is assocciated with cell health and availability of glucose., and LacI, the Lac inhibitor <bb_part>BBa_C0010</bb_part> which binds in an dimerized cooperative manner to inhibit the transcription of the protein that follows. In the presence of lactose or IPTG, an analog of lactose, LacI is unable to correctly bind and inhibit transcription. This allows <bb_part>BBa_R0010</bb_part> to be used as a inverter or as a detector of lactose or IPTG. false true _1_ 0 24 7 In stock false <P> <P><P> LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and this regulator. This part is incompatible with environments containing lactose or lactose analogs. true annotation1961222 1 BBa_R0010 range1961222 1 1 200 annotation1961221 1 end of LacI coding region (inactive) range1961221 1 1 88 annotation1961225 1 -10 range1961225 1 161 166 annotation1961226 1 LacI binding site range1961226 1 166 200 annotation1961224 1 -35 range1961224 1 137 142 annotation1961223 1 CAP binding site range1961223 1 89 126 annotation1961227 1 start range1961227 1 173 173 BBa_K392044 1 BBa_K392044 beta-glucosidase/CBM secretion testing construct for E. coli 2010-10-24T11:00:00Z 2015-05-08T01:12:21Z Assembly of R0010, B0034, J32015, K392008, K392014, B0010. Device containing C. fermi beta-glucosidase fused with cellulose-binding motif CM10; E. coli PelB export tag attached upstream to enable secretion of protein. false false _528_ 0 6220 9 It's complicated false Assembly included Silver standard-compatible parts. Sequence shown on part's main page may not be accurate since it assumes Standard assembly. Please check sequencing results from Get This Part page instead. false Takuro A. Kagaya, Yuki Miyatake, Hirayama Ikumi component2100226 1 BBa_J32015 component2100228 1 BBa_K392014 component2100224 1 BBa_B0034 component2100229 1 BBa_B0010 component2100227 1 BBa_K392008 component2100216 1 BBa_R0010 annotation2100216 1 BBa_R0010 range2100216 1 1 200 annotation2100226 1 BBa_J32015 range2100226 1 227 292 annotation2100227 1 BBa_K392008 range2100227 1 299 1969 annotation2100228 1 BBa_K392014 range2100228 1 1978 3060 annotation2100224 1 BBa_B0034 range2100224 1 209 220 annotation2100229 1 BBa_B0010 range2100229 1 3069 3148 BBa_K392014 1 BBa_K392014 C. josui CM10 2010-10-24T11:00:00Z 2015-05-08T01:12:20Z Xyn10 plasmid received from Shuichi Karita of Faculty of Bioresources, Mie University (Japan). Cellulose-binding motif from C. josui Xyn10A gene, cloned into Silver standard-compatible part. false false _528_ 0 6220 9 It's complicated true Cloned from original plasmid by PCR. false Tadashi Nakamura, Shuhei Yasumoto, Takahiro Saka, Saya Kakuda BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_J32015 1 PelB PelB leader sequence; directs protein to E. coli periplasmic membrane 2006-08-24T11:00:00Z 2015-08-31T04:08:46Z Novagen Released HQ 2013 The pelB leader sequence is a sequence of amino acids which when attached to a protein, directs the protein to the periplasmic membrane of E. coli, where the sequence is removed by pelB peptidase. It is used to direct coat protein-antigen fusions to the cell surface. false true _50_ 0 495 50 In stock true . true Austen Heinz annotation1898182 1 PelB range1898182 1 1 66 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K392008 1 BBa_K392008 C. firmi beta-glucosidase (Silver standard compatible) 2010-10-23T11:00:00Z 2015-05-08T01:12:20Z Original part received from Edinburgh team. Beta-glucosidase from C. firmi re-cloned into Silver standard-compatible part. false false _528_ 0 6220 9 It's complicated true Cloned from original part (Edinburgh) by PCR. false Damian Barnard, Teoh Shao Thing, Tadashi Nakamura BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_R0010_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacaca BBa_B0034_sequence 1 aaagaggagaaa BBa_K392014_sequence 1 attgacgatattagctttgaacaaacgggttcaggaccaatcaaaatccaagatgatttaaaatccattaaagatgtatataaaaaggatttccttattggaaacgcaatagcagcagaggatatggaaggggtcagacttgacctgttaaagaagcactttaatgtaatgacagctggtaatgctatgaagccagatgcattacaaccttcaaagggtaactttacatttgaagatgcagataagcttgttgataaggttctggcagagggattcaagatgcatggacatactcttgtttggcatcagcagtcacctgcttggttaaatacaaaagttgatgcaagcggaaatgctatacctttgtcacgtgaggaagctcttacaaatctaagaacccatattaagacagttgtagagcactacggtaacaaggtaatatcatgggacgtagtaaatgaaggtatgaatgataatcctactaatcctgctgactggaaagcatcattacgtcaaagcccatggtatcaggcaattggtcctgattacatagaagaggcgtttttagcagcaagagaggttctggatgctcaccctgattgggatgtgaagctatattataatgattataacgatgataatcaaagcaaatctaaagctatttactacatggtaaaagagttaaatgaaaaatatgcaaaaactcaccctggtaagcttcttatcgatggagttggtatgcaggcacattacagcttatcaacaaatcctaccaatgttgagctttcattggagagatttatttctctgggtgttgaagtaagcataaccgagcttgatgtacaggcaggcagtgacttcaagttaactgatgaaattgcagaagcacagggatatttatacgcacagttatttgatatatataagaaacatgcagctaacataagccgtgttactatttggggattggatgacggcacaagctggagatcatctacaaatcctttgccatttgacaagaatcttcaggcaaagctcgcatttttcggtttaatagaccctgctaagtacatggatgaacat BBa_J32015_sequence 1 atgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggcc BBa_K392044_sequence 1 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgaaatacctgctgccgaccgctgctgctggtctgctgctcctcgctgcccagccggcgatggcctactagatgggcgaccggttccagcaggccggtcgcccacgccgccgcggcccggcgagggccgttaaccgtaccggtcaagaagacgcgtcgacggggtcgagggagcggtcccacgcgtgtatcgtatcgtttcgacaccgccacccggccaccgggcacgcaccggggacgcagcagtccccgccccggccaccccctgtcaccgaaacccgaaggaccctcatgaccaccacgcgcccctcgggccgccagttctccgacgacttcctgtggggctcggccacggcctcctaccagatcgagggcgcgcacgacgagggcggtcgcggcccgtcgatctgggacacgttctcccgcaccccgggcaaggtcctcaacggcgacaccggcgacgtcgcggtcgaccactaccaccgcgtccccgaggacgtcgagatcatgaagagcctcggcctccaggcctaccggttctcgatcgcctggccccgcatccagccgaccggctcgggcgagttcaaccaggccgggctcgacttctactccgacctcgtcgaccggctcatcgccgccggcatcaagcccgtcgcgacgctgtaccactgggacctcccgcagcccctggaggacgagggcggctgggcgaaccgcgcgacggcgtaccggttcgtcgagtacgcgcgcaagctcgccgaggtcctcggcaagcgcgtcgacctgtggaccacgctcaacgagccgtggtgctccgcgttcctcggctacgcctcaggcgtgcacgccccggcgtcaccgacccggtcaaggccctgcgcgccgtccaccacctcaacctcgcgcacggcctcgcgggccgcgcgatccgcgaggtcctcggcgagaacgcgcccgtctcgatcacgtgaacctgcacgtgacgcgcgccgccgacgactccgccgactccgtcgaggccaagcgccgcatcgacaccatcgcgaacgaggtcttcctcggcccgctgctcgacggcgagtaccccaaggaggtcttcgcggacaccgcgcaccacaccgactggtccttcgtcgagccgggcgacctcgagctcatccgcatcccgctcaccgtgctcggcgtcaactactactcgaccggccgcgtgaagaagggcagcgcgcgggcgccgacccgggcaagccgggccccgacggccaccgcgcctccgagcactcctcgtgggtcggcgccgacgaggtcgagtggctcccgcagcccggcccgcacaccgcgatgggctggaacatcgagcccgacggcctcgtcgacctgctgctcgagctgcgcgaccgttaccccagccagcccctggcgatcacggagaacggtgccgcgttctacgacacggtcagcgaggacggccgcgtgcacgaccccgagcgcgtcggctacctgcacgaccacgtcgacgccgtcggcgaggccatcgacaagggcgccgacgtgcgcgcctacttcgtgtggtcgctgctcgacaacttcgagtggcgctacggctacgaccgccggttcggcatcgtgcgcgtcgactacgacacgcacgagcgcatcgtgaaggactcgggcctctggtaccgcgagctcgtgcgcacccgcaccatcgctccggccgaggacgccgcgacctaccagccctgatactagagattgacgatattagctttgaacaaacgggttcaggaccaatcaaaatccaagatgatttaaaatccattaaagatgtatataaaaaggatttccttattggaaacgcaatagcagcagaggatatggaaggggtcagacttgacctgttaaagaagcactttaatgtaatgacagctggtaatgctatgaagccagatgcattacaaccttcaaagggtaactttacatttgaagatgcagataagcttgttgataaggttctggcagagggattcaagatgcatggacatactcttgtttggcatcagcagtcacctgcttggttaaatacaaaagttgatgcaagcggaaatgctatacctttgtcacgtgaggaagctcttacaaatctaagaacccatattaagacagttgtagagcactacggtaacaaggtaatatcatgggacgtagtaaatgaaggtatgaatgataatcctactaatcctgctgactggaaagcatcattacgtcaaagcccatggtatcaggcaattggtcctgattacatagaagaggcgtttttagcagcaagagaggttctggatgctcaccctgattgggatgtgaagctatattataatgattataacgatgataatcaaagcaaatctaaagctatttactacatggtaaaagagttaaatgaaaaatatgcaaaaactcaccctggtaagcttcttatcgatggagttggtatgcaggcacattacagcttatcaacaaatcctaccaatgttgagctttcattggagagatttatttctctgggtgttgaagtaagcataaccgagcttgatgtacaggcaggcagtgacttcaagttaactgatgaaattgcagaagcacagggatatttatacgcacagttatttgatatatataagaaacatgcagctaacataagccgtgttactatttggggattggatgacggcacaagctggagatcatctacaaatcctttgccatttgacaagaatcttcaggcaaagctcgcatttttcggtttaatagaccctgctaagtacatggatgaacattactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K392008_sequence 1 atgggcgaccggttccagcaggccggtcgcccacgccgccgcggcccggcgagggccgttaaccgtaccggtcaagaagacgcgtcgacggggtcgagggagcggtcccacgcgtgtatcgtatcgtttcgacaccgccacccggccaccgggcacgcaccggggacgcagcagtccccgccccggccaccccctgtcaccgaaacccgaaggaccctcatgaccaccacgcgcccctcgggccgccagttctccgacgacttcctgtggggctcggccacggcctcctaccagatcgagggcgcgcacgacgagggcggtcgcggcccgtcgatctgggacacgttctcccgcaccccgggcaaggtcctcaacggcgacaccggcgacgtcgcggtcgaccactaccaccgcgtccccgaggacgtcgagatcatgaagagcctcggcctccaggcctaccggttctcgatcgcctggccccgcatccagccgaccggctcgggcgagttcaaccaggccgggctcgacttctactccgacctcgtcgaccggctcatcgccgccggcatcaagcccgtcgcgacgctgtaccactgggacctcccgcagcccctggaggacgagggcggctgggcgaaccgcgcgacggcgtaccggttcgtcgagtacgcgcgcaagctcgccgaggtcctcggcaagcgcgtcgacctgtggaccacgctcaacgagccgtggtgctccgcgttcctcggctacgcctcaggcgtgcacgccccggcgtcaccgacccggtcaaggccctgcgcgccgtccaccacctcaacctcgcgcacggcctcgcgggccgcgcgatccgcgaggtcctcggcgagaacgcgcccgtctcgatcacgtgaacctgcacgtgacgcgcgccgccgacgactccgccgactccgtcgaggccaagcgccgcatcgacaccatcgcgaacgaggtcttcctcggcccgctgctcgacggcgagtaccccaaggaggtcttcgcggacaccgcgcaccacaccgactggtccttcgtcgagccgggcgacctcgagctcatccgcatcccgctcaccgtgctcggcgtcaactactactcgaccggccgcgtgaagaagggcagcgcgcgggcgccgacccgggcaagccgggccccgacggccaccgcgcctccgagcactcctcgtgggtcggcgccgacgaggtcgagtggctcccgcagcccggcccgcacaccgcgatgggctggaacatcgagcccgacggcctcgtcgacctgctgctcgagctgcgcgaccgttaccccagccagcccctggcgatcacggagaacggtgccgcgttctacgacacggtcagcgaggacggccgcgtgcacgaccccgagcgcgtcggctacctgcacgaccacgtcgacgccgtcggcgaggccatcgacaagggcgccgacgtgcgcgcctacttcgtgtggtcgctgctcgacaacttcgagtggcgctacggctacgaccgccggttcggcatcgtgcgcgtcgactacgacacgcacgagcgcatcgtgaaggactcgggcctctggtaccgcgagctcgtgcgcacccgcaccatcgctccggccgaggacgccgcgacctaccagccctga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z