LOCUS       BBa_K392047             1775 bp    DNA     linear       05-Nov-2024
DEFINITION  exoglucanase expression device
ACCESSION   BBa_K392047
VERSION     BBa_K392047.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..1684
                     /label=cex coding sequence
     misc_feature    1007..1012
                     /label=PstI site removed
     CDS             227..1687
                     /label=BBa_K118022
     stem_loop       1707..1750
                     /label=stem_loop
     misc_feature    1696..1775
                     /label=BBa_B0010
     terminator      1696..1775
                     /label=BBa_B0010
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc ctaggaccac
      241 gcccgcaccc ggccacccgg cccgcggcgc ccgcaccgct ctgcgcacga cgctcgccgc
      301 cgcggcggcg acgctcgtcg tcggcgccac ggtcgtgctg cccgcccagg ccgcgaccac
      361 gctcaaggag gccgccgacg gcgccggccg ggacttcggc ttcgcgctcg accccaaccg
      421 gctctcggag gcgcagtaca aggcgatcgc cgacagcgag ttcaacctcg tcgtcgccga
      481 gaacgcgatg aagtgggacg ccaccgagcc ctcgcagaac agcttctcct tcggcgcggg
      541 cgaccgcgtc gcgagctacg ccgccgacac cggcaaggag ctgtacggcc acacgctcgt
      601 atggcactcg cagctgcccg actgggcgaa gaacctcaac ggctccgcgt tcgagagcgc
      661 gatggtcaac cacgtgacga aggtcgccga ccacttcgag ggcaaggtcg cgtcgtggga
      721 cgtcgtcaac gaggcgttcg ccgacggcgg cggccgccgg caggactcgg cgttccagca
      781 gaagctcggc aacggctaca tcgagaccgc gttccgggcg gcacgtgcgg cggacccgac
      841 cgccaagctg tgcatcaacg actacaacgt cgagggcatc aacgcgaaga gcaactcgct
      901 ctacgacctc gtcaaggact tcaaggcgcg cggcgtcccg ctcgactgcg tcgggttcca
      961 gtcgcacctc atcgtcggcc aggtgccggg cgacttccgg cagaacctgc aacggttcgc
     1021 ggacctgggc gtggacgtgc gcatcaccga gctcgacatc cgcatgcgga cgccctccga
     1081 cgcgaccaag ctcgcgaccc aggcggccga ctacaagaag gtcgtgcagg cctgcatgca
     1141 ggtgacccgc tgccagggcg tgaccgtctg gggcatcacc gacaagtact cgtgggtgcc
     1201 ggacgtcttc ccgggcgagg gggccgcgct ggtgtgggac gcgagctacg ccaagaagcc
     1261 ggcctacgcc gccgtgatgg aggccttcgg cgcgagcccg acgccgacgc ccaccacgcc
     1321 gaccccgacg cccacgacgc cgacgccgac cccgacgtcc ggtccggccg ggtgccaggt
     1381 gctgtggggc gtcaaccagt ggaacaccgg cttcaccgcg aacgtcaccg tgaagaacac
     1441 gtcctccgct ccggtcgacg gctggacgct cacgttcagc ttcccgtccg gccagcaggt
     1501 cacccaggcg tggagctcga cggtcacgca gtccggctcg gccgtgacgg tccgcaacgc
     1561 cccgtggaac ggctcgatcc cggcgggcgg caccgcgcag ttcggcttca acggctcgca
     1621 cacgggcacc aacgccgcgc cgacggcgtt ctcgctcaac ggcacgccct gcacggtcgg
     1681 ctaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     1741 ttcgttttat ctgttgtttg tcggtgaacg ctctc
//