BBa_K394000 1 BBa_K394000 Resistance Gene for G418 in Yeast 2010-10-17T11:00:00Z 2015-05-08T01:12:21Z The part was synthesized from the PMKV005 plasmid used in McCusker et. al. The coding region contains the KanR open reading from transposon Tn903 from E. coli. 1. McCusker JH, Vorachek-Warren MK. DsdA (D-serine deaminase): a new heterologous MX cassette for gene disruption and selection in Saccharomyces cerevisiae. Yeast (Chichester, England). 2004;21(2):163-71. Available at: http://www.ncbi.nlm.nih.gov/pubmed/14755641. This part confers resistance to Geneticin (G418) in yeast. Selection of yeast cells is typically preformed on plates with 200??g/ml of G418. false false _504_ 0 6327 9 It's complicated true None false Brian Landry annotation2104021 1 KanR gene range2104021 1 1 810 annotation2104022 1 HindIII cut site range2104022 1 545 549 annotation2104023 1 Stop range2104023 1 808 813 annotation2104020 1 Start Codon range2104020 1 1 3 BBa_K394000_sequence 1 atgggtaaggaaaagactcacgtttcgaggccgcgattaaattccaacatggatgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgattgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtcagactaaactggctgacggaatttatgcctcttccgaccatcaagcattttatccgtactcctgatgatgcatggttactcaccactgcgatccccggcaaaacagcattccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggcagtgttcctgcgccggttgcattcgattcctgtttgtaattgtccttttaacagcgatcgcgtatttcgtctcgctcaggcgcaatcacgaatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcataagcttttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgagtcggaatcgcagaccgataccaggatcttgccatcctatggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z