LOCUS       BBa_K394001             3457 bp    DNA     linear       22-Dec-2024
DEFINITION  Plasmid for Chromosomal Integration in Yeast at His3
ACCESSION   BBa_K394001
VERSION     BBa_K394001.1  
FEATURES             Location/Qualifiers
     misc_feature    1..21
                     /label=BioBrick Suffix
     misc_feature    22..69
                     /label=His3 homology
     primer_bind     157..176
                     /label=VR
     CDS             261..875
                     /label=rep (pMB1) (rev)
     misc_feature    276..276
                     /label=ORI
     CDS             1127..2317
                     /label=Tet-R
     CDS             2324..3184
                     /label=AmpR (rev)
     primer_bind     3307..3326
                     /label=VF2
     misc_feature    3386..3435
                     /label=His3 homology
     misc_feature    3435..3457
                     /label=BioBrick Prefix
     misc_feature    71..76
                     /label=BbsI Cut Site
     misc_feature    3379..3385
                     /label=BbsI Cut Site
ORIGIN
        1 tactagtagc ggccgctgca gagagggaga agcagtagca gaacaggcca cacaatcgca
       61 agtgattaac gaagacgatt acttcgcgtt atgcaggctt cctcgctcac tgactcgctg
      121 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
      181 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
      241 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
      301 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
      361 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
      421 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
      481 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
      541 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
      601 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
      661 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta
      721 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
      781 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
      841 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
      901 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
      961 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     1021 tggtctgaca gctcgagatt ctcatgtttg acagcttatc atcgataagc tttaatgcgg
     1081 tagtttatca cagttaaatt gctaacgcag tcaggcaccg tgtatgaaat ctaacaatgc
     1141 gctcatcgtc atcctcggca ccgtcaccct ggatgctgta ggcataggct tggttatgcc
     1201 ggtactgccg ggcctcttgc gggatatcgt ccattccgac agcatcgcca gtcactatgg
     1261 cgtgctgcta gcgctatatg cgttgatgca atttctatgc gcacccgttc tcggagcact
     1321 gtccgaccgc tttggccgcc gcccagtcct gctcgcttcg ctacttggag ccactatcga
     1381 ctacgcgatc atggcgacca cacccgtcct gtggatcctc tacgccggac gcatcgtggc
     1441 cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg
     1501 ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc
     1561 aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc
     1621 ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa
     1681 gggagagcgt cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc
     1741 gcggggcatg actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg
     1801 acaggtgccg gcagcgctct gggtcatttt cggcgaggac cgctttcgct ggagcgcgac
     1861 gatgatcggc ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc aagccttcgt
     1921 cactggtccc gccaccaaac gtttcggcga gaagcaggcc attatcgccg gcatggcggc
     1981 cgacgcgctg ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat
     2041 tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag
     2101 gcaggtagat gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcct
     2161 aacttcgatc actggaccgc tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg
     2221 gaacgggttg gcatggattg taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg
     2281 tcgcggtgca tggagccggg ccacctcgac ctaactcgag ttaccaatgc ttaatcagtg
     2341 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     2401 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     2461 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     2521 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     2581 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     2641 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     2701 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     2761 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     2821 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     2881 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     2941 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     3001 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     3061 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     3121 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     3181 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     3241 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     3301 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
     3361 gtatcacgag gcagaatttc agaagtgatc atcaccgtag tgagagtgcg ttcaaggctc
     3421 ttgcggttgc cataagaatt cgcggccgct tctagag
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