LOCUS       BBa_K394002             3458 bp    DNA     linear       01-Jun-2024
DEFINITION  Plasmid for Chromosomal Integration in Yeast at Ura3
ACCESSION   BBa_K394002
VERSION     BBa_K394002.1  
FEATURES             Location/Qualifiers
     misc_feature    1..21
                     /label=BioBrick Suffix
     misc_feature    22..69
                     /label=Ura3 homology
     primer_bind     157..176
                     /label=VR
     CDS             261..875
                     /label=rep (pMB1) (rev)
     misc_feature    276..276
                     /label=ORI
     CDS             1127..2317
                     /label=Tet-R
     CDS             2324..3184
                     /label=AmpR (rev)
     primer_bind     3307..3326
                     /label=VF2
     misc_feature    3387..3435
                     /label=Ura3 homology
     misc_feature    3435..3457
                     /label=BioBrick Prefix
     misc_feature    3381..3386
                     /label=BbsI Cut Site
     misc_feature    72..77
                     /label=BbsI Cut Site	
ORIGIN
        1 tactagtagc ggccgctgca gtcttaaccc aactgcacag aacaataacc tgctggaaac
       61 gaagataaat cgaagacgat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct
      121 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
      181 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
      241 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
      301 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
      361 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
      421 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
      481 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
      541 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
      601 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
      661 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
      721 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
      781 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
      841 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
      901 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
      961 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     1021 ttggtctgac agctcgagat tctcatgttt gacagcttat catcgataag ctttaatgcg
     1081 gtagtttatc acagttaaat tgctaacgca gtcaggcacc gtgtatgaaa tctaacaatg
     1141 cgctcatcgt catcctcggc accgtcaccc tggatgctgt aggcataggc ttggttatgc
     1201 cggtactgcc gggcctcttg cgggatatcg tccattccga cagcatcgcc agtcactatg
     1261 gcgtgctgct agcgctatat gcgttgatgc aatttctatg cgcacccgtt ctcggagcac
     1321 tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc gctacttgga gccactatcg
     1381 actacgcgat catggcgacc acacccgtcc tgtggatcct ctacgccgga cgcatcgtgg
     1441 ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg
     1501 gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg
     1561 caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg
     1621 cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata
     1681 agggagagcg tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg
     1741 cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag
     1801 gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga
     1861 cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg
     1921 tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg
     1981 ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca
     2041 ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca
     2101 ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc
     2161 taacttcgat cactggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat
     2221 ggaacgggtt ggcatggatt gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc
     2281 gtcgcggtgc atggagccgg gccacctcga cctaactcga gttaccaatg cttaatcagt
     2341 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
     2401 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
     2461 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
     2521 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
     2581 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
     2641 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
     2701 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
     2761 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
     2821 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
     2881 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
     2941 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
     3001 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
     3061 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
     3121 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
     3181 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
     3241 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
     3301 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
     3361 cgtatcacga ggcagaattt cagaaggata agcaggctgg gaagcatatt tgagaagatg
     3421 cggccagcaa tactaagaat tcgcggccgc ttctagag
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